PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1778 [new locus tag: NWMN_RS10165 ]
• pan locus tag^?: SAUPAN001716000
• symbol: NWMN_1778
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1778 [new locus tag: NWMN_RS10165 ]
• symbol: NWMN_1778
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 1990961..1992862
• length: 1902
• essential: unknown

⊟Accession numbers

• Gene ID: 5331065 NCBI
• RefSeq: YP_001332812 NCBI
• BioCyc:
• MicrobesOnline: 3707366 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGACAATAACTATTAAACCACCTAAAGGTAATGGCGCACCTGTACCAGTAGAAACAACT
  TTAGTAAAAAAAGTTAATGCTGACGGTGTATTAACTTTTGATATTCTAGAAAATAAATAT
  ACTTATGAAGTTATTAACGCTATAGGGAAAAGATGGATTGTTAGTCATGTCGAAGGTGAA
  AACGACAAGAAAGAATATGTAATAACTGTCATTGATAGGAAATCAGAAGGCGACAGACAA
  CTGGTTGAATGTACTGCTAGAGAGATTCCTATAGACAAGTTAATGATTGATAGGATTTAT
  GTTAATGTAACAGGATCTTTTACAGTAGAAAGATATTTTAACATTGTGTTTCAAGGTACT
  GGAATGCTTTTTGAAGTCGAAGGTAAGGTTAAGTCTTCGAAGTTTGAAAATGGTGGTGAA
  GGCGACACAAGGTTAGAAATGTTTAAAAAGGGATTAGAACATTTCGGTTTAGAATATAAA
  ATAACGTATGACAAAAAGAAAGACAGATATAAGTTTGTATTGACGCCTTTTGCAAATCAA
  AAAGCGTCTTATTTTATTTCTGACGAAGTCAACGCCAACGCTATAAAACTCGAGGAAGAT
  GCAAGTGATTTCGCCACCTTCATTAGAGGATATGGTAATTATTCAGGAGAAGAAACATTC
  GAACACGCTGGGCTCGTAATGGAAGCTAGAAGCGCATTAGCTGAGATATATGGCGATATC
  CACGCAGAACCATTTAAAGATGGTAAAGTGACTGACCAAGAAACTATGGATAAAGAATTG
  CAATCGAGATTGAAAAAGTCGTTAAAACAATCTTTGTCTTTAGATTTTTTGGTCTTAAGA
  GAATCATATCCAGAAGCAGACCCACAACCCGGAGACATAGTACAAATAAAATCTACCAAA
  CTAGGTTTGAATGATTTAGTCCGTATAGTACAAGTTAAAACGATTAGGGGTATAAACAAT
  GTAATTGTTAAGCAAGATGTAACGCTTGGTGAGTTTAATCGAGAACAACGATATATGAAG
  AAAGTAAATACTGCTGCTAATTATGTTTCTGGATTAAACGATGTTAACCTTTCTAATCCT
  AGTAAAGCGGCAGAAAACTTAAAATCTAAAGTTGCATCAATAGCTAAATCAACACTCGAT
  TTGATGAGTAGAACTGATTTGATTGAAGATAAACAACAGAAGGTAAGCTCTAAAACTGTG
  ACTACATCTGACGGCACTATCGTTCATGATTTTATAGATAAATCAAACATTAAAGATGTA
  AAAACAATTGGAACGATTGGCGATTCTGTAGCTAGAGGATCACATGCGAAAGCAAATTTC
  ACTGAAATGTTAGGCAATAAATTAAAAGCCAAAACAACAAACCTTGCAAGAGGTGGCGCT
  ACAATGGCAACAGTTCCAATAGGTAAAGAAGCGGTAGAAAACAGCATTTATAGACAAGCA
  GAGCAAATAAGAGGAGACCTAATCATATTACAAGGTACAGATGATGACTGGTTACATGGT
  TATTGGGCAGGCGTACCGATAGGCACTGATAAAACGGATACTAAAACGTTTTATGGTGCC
  TTTTGTTCTGCAATTGAAGTTATTCGGAAAAATAATCCAACTTCAAAAATACTTGTAATG
  ACAGCTACTAGACAATGTCCTATGAGTGGCACAACGATACGTCGTAAAGATACTGATAAA
  AACAAATTAGGGTTAACGTTAGAGGATTATGTCAACGCTCAGATATTGGCTTGTAGTGAA
  TTGGATGTACCAGTATATGATGCCTATCATACAGATTATTTTAAGCCATATAATCCAGCG
  TTCAGAAAATCAAGTATGCCAGACGGATTGCATCCGAACGAGAGGGGTCATGAAGTTATT
  ATGTACGAACTTATTAAAAATTATTACCAGTTTTACGGATAG
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⊟Protein

⊟General

• locus tag: NWMN_1778 [new locus tag: NWMN_RS10165 ]
• symbol: NWMN_1778
• description: hypothetical protein
• length: 633
• theoretical pI: 7.06767
• theoretical MW: 70973.1
• GRAVY: -0.452133

⊟Function

• TIGRFAM:
• TheSEED:
• PFAM:
  Phage_barrel (CL0504) Prophage_tail; Prophage endopeptidase tail (PF06605; HMM-score: 78.6)
  and 2 more
  SGNH_hydrolase (CL0264) Lipase_GDSL_2; GDSL-like Lipase/Acylhydrolase family (PF13472; HMM-score: 54.4)
  Lipase_GDSL; GDSL-like Lipase/Acylhydrolase (PF00657; HMM-score: 24)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 0
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.008885
  • TAT(Tat/SPI): 0.000504
  • LIPO(Sec/SPII): 0.000846
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151221990 NCBI
• RefSeq: YP_001332812 NCBI
• UniProt: A0A0H3KDN7 UniProt

⊟Protein sequence

• MTITIKPPKGNGAPVPVETTLVKKVNADGVLTFDILENKYTYEVINAIGKRWIVSHVEGENDKKEYVITVIDRKSEGDRQLVECTAREIPIDKLMIDRIYVNVTGSFTVERYFNIVFQGTGMLFEVEGKVKSSKFENGGEGDTRLEMFKKGLEHFGLEYKITYDKKKDRYKFVLTPFANQKASYFISDEVNANAIKLEEDASDFATFIRGYGNYSGEETFEHAGLVMEARSALAEIYGDIHAEPFKDGKVTDQETMDKELQSRLKKSLKQSLSLDFLVLRESYPEADPQPGDIVQIKSTKLGLNDLVRIVQVKTIRGINNVIVKQDVTLGEFNREQRYMKKVNTAANYVSGLNDVNLSNPSKAAENLKSKVASIAKSTLDLMSRTDLIEDKQQKVSSKTVTTSDGTIVHDFIDKSNIKDVKTIGTIGDSVARGSHAKANFTEMLGNKLKAKTTNLARGGATMATVPIGKEAVENSIYRQAEQIRGDLIILQGTDDDWLHGYWAGVPIGTDKTDTKTFYGAFCSAIEVIRKNNPTSKILVMTATRQCPMSGTTIRRKDTDKNKLGLTLEDYVNAQILACSELDVPVYDAYHTDYFKPYNPAFRKSSMPDGLHPNERGHEVIMYELIKNYYQFYG

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_1775 < NWMN_1776 < NWMN_1777 < NWMN_1778 < NWMN_1779 < NWMN_1780 < NWMN_1781 < NWMN_1782 < NWMN_1783 < NWMN_1784 < NWMN_1785 < NWMN_1786 < NWMN_1787 < NWMN_1788 < NWMN_1789 < NWMN_1790

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications