PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1349 [new locus tag: NWMN_RS07610 ]
• pan locus tag^?: SAUPAN003893000
• symbol: ald
• pan gene symbol^?: ald1
• synonym:
• product: alanine dehydrogenase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1349 [new locus tag: NWMN_RS07610 ]
• symbol: ald
• product: alanine dehydrogenase
• replicon: chromosome
• strand: -
• coordinates: 1511977..1513095
• length: 1119
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5332036 NCBI
• RefSeq: YP_001332383 NCBI
• BioCyc:
• MicrobesOnline: 3706901 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGTTAGTTGCAGTAGTCAAAGAATTAAAACAAGGTGAAGGACGTGTAGCTTGCACACCC
  GAAAATGTGCGTAAGTTAACGGATGCAGGACATAAAGTAATTGTTGAAAAAAATGCTGGC
  ATTGGTTCAGGATTTTCTAACGATATGTATGAAAAAGAAGGCGCTAAGATCGTAACTCAC
  GAACAAGCATGGGAAGCTGATCTTGTTATCAAAGTAAAAGAACCTCATGAAAGCGAATAT
  CAATATTTCAAAAAGAATCAAATTATCTGGGGATTTTTACATCTAGCATCTTCAAAAGAA
  ATAGTAGAAAAAATGCAAGAAGTTGGTGTAACTGCGATTAGTGGTGAAACCATTATAAAA
  AATGGAAAAGCAGAATTATTAGCGCCAATGAGTGCTATAGCAGGTCAACGCTCAGCAATT
  ATGGGAGCTTACTACTCTGAAGCACAACATGGTGGTCAAGGTACTTTAGTGACTGGTGTA
  CATGAAAATGTGGATATACCTGGTAGTACATATGTGATTTTCGGTGGTGGAGTAGCAGCA
  ACAAATGCAGCAAATGTTGCCTTGGGACTAAATGCTAAAGTAATCATTATCGAGTTAAAC
  GATGACCGCATTAAATATCTTGAAGATATGTATGCAGAAAAAGATGTCACAGTAGTCAAA
  TCAACACCAGAAAATTTAGCAGAACAAATTAAGAAAGCAGATGTATTTATTTCTACAATT
  TTAATTCCAGGTGCGAAACCGCCAAAATTGGTTACTCGTGAGATGGTTAAATCAATGAAA
  AAAGGTTCAGTATTAATCGATATAGCTATTGACCAAGGTGGAACTATTGAAACAATTAGA
  CCAACTACAATTTCTGATCCAGTGTATGAAGAAGAAGGTGTGATTCATTATGGTGTACCA
  AATCAACCAGGAGCAGTCCCAAGAACTTCAACAATGGCATTAGCACAAGGAAATATTGAT
  TATATATTAGAAATTTGTGACAAAGGCTTAGAACAAGCAATTAAAGATAATGAAGCCTTA
  AGTACTGGTGTAAACATTTACCAAGGACAAGTGACAAATCAAGGATTAGCTTCATCACAT
  GACCTAGATTATAAAGAAATATTAAATGTTATCGAATAG
  60
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  1119
  

⊟Protein

⊟General

• locus tag: NWMN_1349 [new locus tag: NWMN_RS07610 ]
• symbol: Ald
• description: alanine dehydrogenase
• length: 372
• theoretical pI: 4.89305
• theoretical MW: 40234.7
• GRAVY: -0.140323

⊟Function

• reaction:
  EC 1.4.1.1?  ExPASy
  Alanine dehydrogenase L-alanine + H₂O + NAD⁺ = pyruvate + NH₃ + NADH
• TIGRFAM:
  Metabolism Energy metabolism Amino acids and amines alanine dehydrogenase (TIGR00518; EC 1.4.1.1; HMM-score: 395.5)
  and 6 more
  Metabolism Energy metabolism Electron transport NAD(P)(+) transhydrogenase (AB-specific), alpha subunit (TIGR00561; EC 1.6.1.2; HMM-score: 141.1)
  Cellular processes Cellular processes Sporulation and germination dipicolinic acid synthetase, A subunit (TIGR02853; HMM-score: 23.2)
  Metabolism Energy metabolism Amino acids and amines adenosylhomocysteinase (TIGR00936; EC 3.3.1.1; HMM-score: 18.7)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin glutamyl-tRNA reductase (TIGR01035; EC 1.2.1.70; HMM-score: 16.6)
  methylmalonyl-CoA mutase C-terminal domain (TIGR00640; HMM-score: 15.4)
  Metabolism Amino acid biosynthesis Aromatic amino acid family shikimate-5-dehydrogenase (TIGR01809; EC 1.1.1.25; HMM-score: 15)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  NADP_Rossmann (CL0063) AlaDh_PNT_C; Alanine dehydrogenase/PNT, C-terminal domain (PF01262; HMM-score: 254.1)
  and 11 more
  Form_Glyc_dh (CL0325) AlaDh_PNT_N; Alanine dehydrogenase/PNT, N-terminal domain (PF05222; HMM-score: 142.1)
  NADP_Rossmann (CL0063) 2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 25.8)
  THF_DHG_CYH_C; Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain (PF02882; HMM-score: 24.5)
  Shikimate_DH; Shikimate / quinate 5-dehydrogenase (PF01488; HMM-score: 22.7)
  NAD_binding_7; Putative NAD(P)-binding (PF13241; HMM-score: 20.3)
  Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase (PF00070; HMM-score: 17.2)
  Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 14.9)
  Methyltransf_11; Methyltransferase domain (PF08241; HMM-score: 14.2)
  ADH_zinc_N; Zinc-binding dehydrogenase (PF00107; HMM-score: 14.1)
  TrkA_N; TrkA-N domain (PF02254; HMM-score: 13.9)
  Form_Glyc_dh (CL0325) 2-Hacid_dh; D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain (PF00389; HMM-score: 13.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.011205
  • TAT(Tat/SPI): 0.000954
  • LIPO(Sec/SPII): 0.005196
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151221561 NCBI
• RefSeq: YP_001332383 NCBI
• UniProt: A0A0H3KCR5 UniProt

⊟Protein sequence

• MLVAVVKELKQGEGRVACTPENVRKLTDAGHKVIVEKNAGIGSGFSNDMYEKEGAKIVTHEQAWEADLVIKVKEPHESEYQYFKKNQIIWGFLHLASSKEIVEKMQEVGVTAISGETIIKNGKAELLAPMSAIAGQRSAIMGAYYSEAQHGGQGTLVTGVHENVDIPGSTYVIFGGGVAATNAANVALGLNAKVIIIELNDDRIKYLEDMYAEKDVTVVKSTPENLAEQIKKADVFISTILIPGAKPPKLVTREMVKSMKKGSVLIDIAIDQGGTIETIRPTTISDPVYEEEGVIHYGVPNQPGAVPRTSTMALAQGNIDYILEICDKGLEQAIKDNEALSTGVNIYQGQVTNQGLASSHDLDYKEILNVIE

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_1347 < ilvA < ald

⊟Regulation

• regulator: Rex* (repression) regulon

  Rex*

  (TF)

  important in Energy metabolism; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications