PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1210 [new locus tag: NWMN_RS06805 ]
• pan locus tag^?: SAUPAN003615000
• symbol: NWMN_1210
• pan gene symbol^?: —
• synonym:
• product: hydrolase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1210 [new locus tag: NWMN_RS06805 ]
• symbol: NWMN_1210
• product: hydrolase
• replicon: chromosome
• strand: +
• coordinates: 1336714..1337628
• length: 915
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5331957 NCBI
• RefSeq: YP_001332244 NCBI
• BioCyc:
• MicrobesOnline: 3706762 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGACTGAAAAACAATTTAAATTAACTGTACAAGATAATACGAATATTGAAGTTAAAGTG
  AATTTTACAGATGTAGATTCAAAAGGAATTATTCATATATTTCATGGTATGGCTGAACAT
  ATGGAACGTTACGATAAATTAGCACATGCACTTTCAAAGCATGGCTTCGATGTGATACGT
  CATAATCATCGAGGACATGGTATTAATATTGATGAATCAACAAGAGGGCATTACGATGAT
  ATGAAACGAGTTATCGGTGATGCCTTTGAAGTAGCGCAAACAGTGAGAGGCAATGTTGAT
  AAACCATACATTATAATCGGACATTCAATGGGATCCGTTATAGCTAGATTGTTTGTAGAA
  ACATATCCGCAATATGTTGATGGTCTAATTTTAAGTGGTACTGGTATGTATTCATTATGG
  AAAGGTTTACCAACCGTTAAAGTGTTACAACTGATTACAAAAATTTATGGTGCTGAGAAA
  CGAGTTGAATGGGTTAACCAGTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATTA
  CGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGAT
  CCATATAGTGGATTTAATGTGTCAAATCAATTATTATATCAAACAGCCTATTATATGCTA
  CATACATCACAATTAAAAAATATGAAAATGTTAAATCATGCCATGCCTATATTATTAGTT
  TCAGGATATGACGATCCTTTAGGTGATTATGGTAAAGGGATTTTAAAATTGGCGAATATA
  TATAGAAAAGCTGGCATTAAAAATGTTAAAGTGAATCTTTATCATCATAAACGTCATGAA
  GTGTTATTTGAAAAAGATCATGACAAAATTTGGGAAGACTTGTTTAAATGGTTGAATCAA
  TTTTATAAAAAATAA
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⊟Protein

⊟General

• locus tag: NWMN_1210 [new locus tag: NWMN_RS06805 ]
• symbol: NWMN_1210
• description: hydrolase
• length: 304
• theoretical pI: 9.65305
• theoretical MW: 35255.3
• GRAVY: -0.419408

⊟Function

• TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (TIGR03695; EC 4.2.99.20; HMM-score: 35.4)
  and 5 more
  Metabolism Energy metabolism Other 3-oxoadipate enol-lactonase (TIGR02427; EC 3.1.1.24; HMM-score: 25.2)
  2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (TIGR03343; EC 3.7.1.-; HMM-score: 24.1)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin pimelyl-[acyl-carrier protein] methyl ester esterase (TIGR01738; EC 3.1.1.85; HMM-score: 23.3)
  proline-specific peptidase (TIGR01250; HMM-score: 22.9)
  Metabolism Energy metabolism Photosynthesis putative magnesium chelatase accessory protein (TIGR03056; HMM-score: 14.3)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  AB_hydrolase (CL0028) Hydrolase_4; Serine aminopeptidase, S33 (PF12146; HMM-score: 170.4)
  and 12 more
  Abhydrolase_1; alpha/beta hydrolase fold (PF00561; HMM-score: 72.4)
  Abhydrolase_6; Alpha/beta hydrolase family (PF12697; HMM-score: 29.3)
  Abhydrolase_5; Alpha/beta hydrolase family (PF12695; HMM-score: 18.7)
  Peptidase_S28; Serine carboxypeptidase S28 (PF05577; HMM-score: 17.7)
  LIDHydrolase; Lipid-droplet associated hydrolase (PF10230; HMM-score: 17.4)
  Abhydrolase_2; Phospholipase/Carboxylesterase (PF02230; HMM-score: 16.5)
  PGAP1; PGAP1-like protein (PF07819; HMM-score: 16.2)
  Peptidase_S9; Prolyl oligopeptidase family (PF00326; HMM-score: 15.9)
  Thioesterase; Thioesterase domain (PF00975; HMM-score: 15.8)
  Acyl_transf_2; Acyl transferase (PF02273; HMM-score: 15)
  DUF2920; Protein of unknown function (DUF2920) (PF11144; HMM-score: 15)
  LCAT; Lecithin:cholesterol acyltransferase (PF02450; HMM-score: 14.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.32
  • Cytoplasmic Membrane Score: 9.55
  • Cellwall Score: 0.12
  • Extracellular Score: 0.01
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004024
  • TAT(Tat/SPI): 0.00026
  • LIPO(Sec/SPII): 0.000268
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151221422 NCBI
• RefSeq: YP_001332244 NCBI
• UniProt: A0A0H3KG67 UniProt

⊟Protein sequence

• MTEKQFKLTVQDNTNIEVKVNFTDVDSKGIIHIFHGMAEHMERYDKLAHALSKHGFDVIRHNHRGHGINIDESTRGHYDDMKRVIGDAFEVAQTVRGNVDKPYIIIGHSMGSVIARLFVETYPQYVDGLILSGTGMYSLWKGLPTVKVLQLITKIYGAEKRVEWVNQLVSNSFNKKIRPLRTQSDWISSNPIEVDNFIKDPYSGFNVSNQLLYQTAYYMLHTSQLKNMKMLNHAMPILLVSGYDDPLGDYGKGILKLANIYRKAGIKNVKVNLYHHKRHEVLFEKDHDKIWEDLFKWLNQFYKK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_1210 > miaA > NWMN_1212

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications