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NCBI: 06-JUL-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus Newman
- locus tag: NWMN_1179 [new locus tag: NWMN_RS06650 ]
- pan locus tag?: SAUPAN003571000
- symbol: rbfA
- pan gene symbol?: rbfA
- synonym:
- product: ribosome-binding factor A
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: NWMN_1179 [new locus tag: NWMN_RS06650 ]
- symbol: rbfA
- product: ribosome-binding factor A
- replicon: chromosome
- strand: +
- coordinates: 1298829..1299221
- length: 393
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 5332043 NCBI
- RefSeq: YP_001332213 NCBI
- BioCyc:
- MicrobesOnline: 3706731 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
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361ATGCAAATAACAATTGAACTTAAAGATAAAGAGGTGACAAGAATGAGCAGTATGAGAGCA
GAGCGTGTTGGTGAACAAATGAAGAAGGAATTAATGGATATCATCAACAATAAAGTCAAA
GATCCTCGAGTTGGTTTTATTACAATTACAGATGTTGTTTTAACAAATGATTTATCGCAG
GCTAAAGTATTTTTAACTGTATTAGGTAACGATAAAGAAGTAGAAAATACATTTAAAGCA
CTTGATAAAGCAAAAGGCTTCATTAAGTCTGAATTAGGTTCTAGAATGCGATTACGTATT
ATGCCGGAATTAATGTATGAATATGATCAATCAATCGAATATGGTAATAAAATTGAACGA
ATGATTCAAGATTTACACAAACAAGATAGATAA60
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393
⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: NWMN_1179 [new locus tag: NWMN_RS06650 ]
- symbol: RbfA
- description: ribosome-binding factor A
- length: 130
- theoretical pI: 8.88746
- theoretical MW: 15200.6
- GRAVY: -0.553077
⊟Function[edit | edit source]
- TIGRFAM: Transcription RNA processing ribosome-binding factor A (TIGR00082; HMM-score: 125.5)
- TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
- PFAM: DnaA_N (CL0494) RBFA; Ribosome-binding factor A (PF02033; HMM-score: 111.2)and 1 moreno clan defined DUF4201; Domain of unknown function (DUF4201) (PF13870; HMM-score: 10.7)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.67
- Cytoplasmic Membrane Score: 0.01
- Cellwall Score: 0.15
- Extracellular Score: 0.17
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.006734
- TAT(Tat/SPI): 0.000192
- LIPO(Sec/SPII): 0.00056
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MQITIELKDKEVTRMSSMRAERVGEQMKKELMDIINNKVKDPRVGFITITDVVLTNDLSQAKVFLTVLGNDKEVENTFKALDKAKGFIKSELGSRMRLRIMPELMYEYDQSIEYGNKIERMIQDLHKQDR
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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