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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0735 [new locus tag: NWMN_RS04165 ]
  • pan locus tag?: SAUPAN002692000
  • symbol: NWMN_0735
  • pan gene symbol?: whiA
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0735 [new locus tag: NWMN_RS04165 ]
  • symbol: NWMN_0735
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 827548..828492
  • length: 945
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAGCTTTGCATCAGAAATGAAAAATGAATTAACTAGAATAGACGTCGATGAAATGAAT
    GCAAAAGCAGAGCTCAGTGCACTGATTCGAATGAATGGTGCACTTAGTCTTTCAAATCAA
    CAATTTGTTATAAATGTTCAAACGGAAAATGCAACAACGGCAAGACGTATTTATTCGTTG
    ATTAAACGTGTCTTTAATGTGGAAGTTGAAATATTAGTCCGTAAAAAAATGAAACTTAAA
    AAAAATAATATTTATATTTGTCGTACAAAGATGAAAGCGAAAGAAATTCTTGATGAATTA
    GGAATTTTAAAAGACGGCATTTTTACGCATGAAATTGATCATTCAATGATTCAAGATGAC
    GAAATGAGACGCAGTTACTTGAGAGGAGCTTTTCTGGCAGGTGGCTCAGTGAATAACCCT
    GAAACATCTTCGTACCATTTGGAAATTTTTTCTCAAAATGAGAGTCATGCAGAAGGCTTA
    ACGAAACTAATGAATAGTTATGAGTTGAATGCCAAACATTTAGAGCGAAAAAAAGGAAGT
    ATTACGTATTTAAAAGAAGCGGAAAAGATTTCGGATTTTCTTAGTTTGATAGGTGGCTAT
    CAAGCGTTATTAAAATTTGAAGACGTACGTATTGTAAGAGATATGCGTAATTCTGTTAAC
    CGACTCGTTAATTGTGAAACGGCCAATCTAAATAAAACAGTTAGTGCTGCGATGAAACAA
    GTTGAGAGCATTAAATTGATTGATAAAGAAATTGGTATTGAAAATTTACCAGACAGGTTG
    AGAGAGATTGCTAGAATTCGAGTAGAACATCAAGAAATTTCGTTGAAAGAGCTTGGAGAA
    ATGGTATCAACTGGTCCAATTTCAAAATCAGGTGTAAATCACCGATTAAGAAAACTGAAT
    GATTTAGCCGATAAGATTAGAAATGGTGAACAAATAGAATTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    945

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_0735 [new locus tag: NWMN_RS04165 ]
  • symbol: NWMN_0735
  • description: hypothetical protein
  • length: 314
  • theoretical pI: 9.22195
  • theoretical MW: 35868.1
  • GRAVY: -0.422611

⊟Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Cell division DNA-binding protein WhiA (TIGR00647; HMM-score: 447.1)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    Homing_endonuc (CL0324) LAGLIDADG_WhiA; WhiA LAGLIDADG-like domain (PF14527; HMM-score: 125.3)
    HTH (CL0123) HTH_WhiA; WhiA C-terminal HTH domain (PF02650; HMM-score: 121.2)
    Homing_endonuc (CL0324) WhiA_N; WhiA N-terminal LAGLIDADG-like domain (PF10298; HMM-score: 118.2)
    and 2 more
    LAGLIDADG_3; LAGLIDADG-like domain (PF14528; HMM-score: 16.7)
    no clan defined DUF3486; Protein of unknown function (DUF3486) (PF11985; HMM-score: 14.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003018
    • TAT(Tat/SPI): 0.000921
    • LIPO(Sec/SPII): 0.000446
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSFASEMKNELTRIDVDEMNAKAELSALIRMNGALSLSNQQFVINVQTENATTARRIYSLIKRVFNVEVEILVRKKMKLKKNNIYICRTKMKAKEILDELGILKDGIFTHEIDHSMIQDDEMRRSYLRGAFLAGGSVNNPETSSYHLEIFSQNESHAEGLTKLMNSYELNAKHLERKKGSITYLKEAEKISDFLSLIGGYQALLKFEDVRIVRDMRNSVNRLVNCETANLNKTVSAAMKQVESIKLIDKEIGIENLPDRLREIARIRVEHQEISLKELGEMVSTGPISKSGVNHRLRKLNDLADKIRNGEQIEL

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SigB (activation) regulon
    SigB(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation;  [1]   other strains

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J Bacteriol: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)

⊟Relevant publications[edit | edit source]

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