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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0411 [new locus tag: NWMN_RS02320 ]
  • pan locus tag?: SAUPAN002145000
  • symbol: lpl8nm
  • pan gene symbol?: lpl8
  • synonym:
  • product: tandem lipoprotein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0411 [new locus tag: NWMN_RS02320 ]
  • symbol: lpl8nm
  • product: tandem lipoprotein
  • replicon: chromosome
  • strand: +
  • coordinates: 459968..460753
  • length: 786
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGAAGTCTATAAAAAGGATTGGATTGTGCATTAGTTTGTTGATTTTAATCATCTTTGTT
    ACATCTTGTGATGGTGATAATAAGATCATTGGAGATTCAAAAGAAGAACAAATCAAAAAG
    AGCTTTGCGAAAACGTTAGATATCTACCCTATTAAGAATCTCGAGGATTTATACGACAAA
    GAAGGATATCGAGATGGCGAATTTAAAAAAGATGATAAAGGTACTTGGCTAATTAGATCT
    GAAATGAAAATCCAATTAAAAGGAGAAAATCTGGAATCTAGAGGAGCAGTTTTAGAAATT
    AACAGAAATACTAGAACGGCTAAAGGGCATTATATTGTTAGAGAAGTTGTTGAAGATAGC
    GATGGAATGACACACAATCATACAAAAAGATATCCTGTGAAAATGGAAAATAATAAAATG
    ATTCCATTAAAACCAATCGATGACGAAAAAGTAAAAAAAGAAATCGAAGAATTTAACTTC
    TTTGTACAATACGGGAATTTCAAAGAATTGGAAAACTATAAAGAGGACGAAGTGTCATAT
    AACCCAGAAGTACCAATTTACTCTGCAAAATATCAATTGAAAAACAGTGATTACAATGTT
    GAACAATTAAGGAAGCGATATAATATCCCGACGCAAAAAGCGCCCAAATTATTATTGAAA
    GGCTCAGGTAATTTAAAAGGTTCATCAGTCGGATATAAAAATATTGAATTTACCTTTATT
    GAAAACAAGGAAGAAAATATTTACTTCACAGATAGTATCTACTTTAATCCAAGCGAGGAT
    AAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    786

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_0411 [new locus tag: NWMN_RS02320 ]
  • symbol: Lpl8nm
  • description: tandem lipoprotein
  • length: 261
  • theoretical pI: 7.48244
  • theoretical MW: 30502.5
  • GRAVY: -0.796552

⊟Function[edit | edit source]

  • TIGRFAM:
    Staphylococcus tandem lipoproteins (TIGR01742; HMM-score: 361.6)
  • TheSEED  :
    • FIG01107840: hypothetical protein
  • PFAM:
    LolA_LolB (CL0048) DUF576; Csa1 family (PF04507; HMM-score: 382.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 4.6
    • Cellwall Score: 2.48
    • Extracellular Score: 2.92
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.0006
    • Cytoplasmic Membrane Score: 0.5293
    • Cell wall & surface Score: 0.0025
    • Extracellular Score: 0.4676
  • LocateP: Lipid anchored
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPII)
    • Intracellular possibility: 0
    • Signal peptide possibility: 0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: IFVTSCD
  • SignalP: Signal peptide LIPO(Sec/SPII) length 22 aa
    • SP(Sec/SPI): 0.001295
    • TAT(Tat/SPI): 0.000037
    • LIPO(Sec/SPII): 0.998504
    • Cleavage Site: CS pos: 22-23. VTS-CD. Pr: 0.9990
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKSIKRIGLCISLLILIIFVTSCDGDNKIIGDSKEEQIKKSFAKTLDIYPIKNLEDLYDKEGYRDGEFKKDDKGTWLIRSEMKIQLKGENLESRGAVLEINRNTRTAKGHYIVREVVEDSDGMTHNHTKRYPVKMENNKMIPLKPIDDEKVKKEIEEFNFFVQYGNFKELENYKEDEVSYNPEVPIYSAKYQLKNSDYNVEQLRKRYNIPTQKAPKLLLKGSGNLKGSSVGYKNIEFTFIENKEENIYFTDSIYFNPSEDK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SaeR (activation) regulon
    SaeR(TF)important in Virulence; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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