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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0056 [new locus tag: NWMN_RS00320 ]
  • pan locus tag?: SAUPAN000910000
  • symbol: sarH1
  • pan gene symbol?: sarS
  • synonym:
  • product: accessory regulator A-like protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0056 [new locus tag: NWMN_RS00320 ]
  • symbol: sarH1
  • product: accessory regulator A-like protein
  • replicon: chromosome
  • strand: -
  • coordinates: 75375..76151
  • length: 777
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    GTGCATATACAAGGAGATAAAACAATGAAATATAATAACCATGACAAAATTAGAGATTTT
    ATAATCATTGAAGCATATATGTTTCGTTTTAAGAAAAAAGTCAAGCCTGAAGTCGATATG
    ACTATAAAAGAATTTATATTACTGACTTATTTATTTCATCAGCAAGAAAACACACTTCCA
    TTTAAGAAGATTGTTTCAGATTTATGTTATAAACAATCGGATTTAGTACAGCATATAAAA
    GTACTTGTGAAACATTCATATATTAGTAAAGTTCGAAGTAAAATTGATGAGCGTAATACT
    TACATTTCAATATCTGAAGAACAACGAGAGAAAATTGCAGAACGTGTTACATTGTTTGAT
    CAAATCATTAAACAATTTAACCTTGCAGATCAAAGTGAATCACAGATGATACCAAAAGAT
    AGTAAAGAATTTCTAAACTTGATGATGTATACAATGTATTTCAAGAATATTATCAAAAAA
    CATCTAACATTAAGTTTTGTAGAATTCACAATTCTAGCTATTATCACTTCTCAAAATAAA
    AACATCGTTCTTCTTAAAGATTTAATTGAAACAATCCACCATAAATACCCTCAAACTGTT
    AGAGCTCTCAATAATTTAAAAAAGCAAGGCTATCTAATAAAAGAACGCTCAACTGAAGAT
    GAAAGAAAAATTTTAATTCATATGGATGACGCGCAGCAAGACCATGCTGAACAATTATTA
    GCTCAAGTGAATCAATTATTAGCAGATAAAGATCATTTACATCTTGTTTTTGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    777

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_0056 [new locus tag: NWMN_RS00320 ]
  • symbol: SarH1
  • description: accessory regulator A-like protein
  • length: 258
  • theoretical pI: 9.30531
  • theoretical MW: 30800.7
  • GRAVY: -0.417442

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    Signal transduction Regulatory functions DNA interactions staphylococcal accessory regulator family (TIGR01889; HMM-score: 214.5)
    and 4 more
    homoprotocatechuate degradation operon regulator, HpaR (TIGR02337; HMM-score: 33)
    mobile rSAM pair MarR family regulator (TIGR04472; HMM-score: 14.7)
    Signal transduction Regulatory functions DNA interactions heavy metal response regulator (TIGR01387; HMM-score: 14.3)
    Genetic information processing Mobile and extrachromosomal element functions Prophage functions putative phage terminase, small subunit, P27 family (TIGR01558; HMM-score: 13.5)
  • TheSEED  :
    • Staphylococcal accessory regulator S, SarS
  • ⊞PFAM:
    HTH (CL0123) Staph_reg_Sar_Rot; Transcriptional regulator SarA/Rot (PF22381; HMM-score: 132.7)
    and 9 more
    MarR_2; MarR family (PF12802; HMM-score: 42.1)
    MarR; MarR family (PF01047; HMM-score: 33.8)
    HTH_27; Winged helix DNA-binding domain (PF13463; HMM-score: 28.9)
    Trans_reg_C; Transcriptional regulatory protein, C terminal (PF00486; HMM-score: 25.8)
    HTH_34; Winged helix DNA-binding domain (PF13601; HMM-score: 19.7)
    HTH_36; Helix-turn-helix domain (PF13730; HMM-score: 14.4)
    MAGE; MAGE homology domain (PF01454; HMM-score: 11.9)
    no clan defined UPF0515; Uncharacterised protein UPF0515 (PF15135; HMM-score: 11.8)
    PsbQ-like (CL0752) CYP38_PsbQ-like; Peptidyl-prolyl cis-trans isomerase CYP38-like, PsbQ-like domain (PF21329; HMM-score: 9.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.8978
    • Cytoplasmic Membrane Score: 0.0715
    • Cell wall & surface Score: 0.0053
    • Extracellular Score: 0.0254
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00274
    • TAT(Tat/SPI): 0.000127
    • LIPO(Sec/SPII): 0.000732
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MHIQGDKTMKYNNHDKIRDFIIIEAYMFRFKKKVKPEVDMTIKEFILLTYLFHQQENTLPFKKIVSDLCYKQSDLVQHIKVLVKHSYISKVRSKIDERNTYISISEEQREKIAERVTLFDQIIKQFNLADQSESQMIPKDSKEFLNLMMYTMYFKNIIKKHLTLSFVEFTILAIITSQNKNIVLLKDLIETIHHKYPQTVRALNNLKKQGYLIKERSTEDERKILIHMDDAQQDHAEQLLAQVNQLLADKDHLHLVFE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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