PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_0056 [new locus tag: NWMN_RS00320 ]
• pan locus tag^?: SAUPAN000910000
• symbol: sarH1
• pan gene symbol^?: sarS
• synonym:
• product: accessory regulator A-like protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0056 [new locus tag: NWMN_RS00320 ]
• symbol: sarH1
• product: accessory regulator A-like protein
• replicon: chromosome
• strand: -
• coordinates: 75375..76151
• length: 777
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5332493 NCBI
• RefSeq: YP_001331091 NCBI
• BioCyc:
• MicrobesOnline: 3705587 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  GTGCATATACAAGGAGATAAAACAATGAAATATAATAACCATGACAAAATTAGAGATTTT
  ATAATCATTGAAGCATATATGTTTCGTTTTAAGAAAAAAGTCAAGCCTGAAGTCGATATG
  ACTATAAAAGAATTTATATTACTGACTTATTTATTTCATCAGCAAGAAAACACACTTCCA
  TTTAAGAAGATTGTTTCAGATTTATGTTATAAACAATCGGATTTAGTACAGCATATAAAA
  GTACTTGTGAAACATTCATATATTAGTAAAGTTCGAAGTAAAATTGATGAGCGTAATACT
  TACATTTCAATATCTGAAGAACAACGAGAGAAAATTGCAGAACGTGTTACATTGTTTGAT
  CAAATCATTAAACAATTTAACCTTGCAGATCAAAGTGAATCACAGATGATACCAAAAGAT
  AGTAAAGAATTTCTAAACTTGATGATGTATACAATGTATTTCAAGAATATTATCAAAAAA
  CATCTAACATTAAGTTTTGTAGAATTCACAATTCTAGCTATTATCACTTCTCAAAATAAA
  AACATCGTTCTTCTTAAAGATTTAATTGAAACAATCCACCATAAATACCCTCAAACTGTT
  AGAGCTCTCAATAATTTAAAAAAGCAAGGCTATCTAATAAAAGAACGCTCAACTGAAGAT
  GAAAGAAAAATTTTAATTCATATGGATGACGCGCAGCAAGACCATGCTGAACAATTATTA
  GCTCAAGTGAATCAATTATTAGCAGATAAAGATCATTTACATCTTGTTTTTGAATAA
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⊟Protein

⊟General

• locus tag: NWMN_0056 [new locus tag: NWMN_RS00320 ]
• symbol: SarH1
• description: accessory regulator A-like protein
• length: 258
• theoretical pI: 9.30531
• theoretical MW: 30800.7
• GRAVY: -0.417442

⊟Function

• TIGRFAM:
  Signal transduction Regulatory functions DNA interactions staphylococcal accessory regulator family (TIGR01889; HMM-score: 214.5)
  and 4 more
  homoprotocatechuate degradation operon regulator, HpaR (TIGR02337; HMM-score: 33)
  mobile rSAM pair MarR family regulator (TIGR04472; HMM-score: 14.7)
  Signal transduction Regulatory functions DNA interactions heavy metal response regulator (TIGR01387; HMM-score: 14.3)
  Genetic information processing Mobile and extrachromosomal element functions Prophage functions putative phage terminase, small subunit, P27 family (TIGR01558; HMM-score: 13.5)
• TheSEED  :
  • Staphylococcal accessory regulator S, SarS
• PFAM:
  HTH (CL0123) Staph_reg_Sar_Rot; Transcriptional regulator SarA/Rot (PF22381; HMM-score: 132.7)
  and 9 more
  MarR_2; MarR family (PF12802; HMM-score: 42.1)
  MarR; MarR family (PF01047; HMM-score: 33.8)
  HTH_27; Winged helix DNA-binding domain (PF13463; HMM-score: 28.9)
  Trans_reg_C; Transcriptional regulatory protein, C terminal (PF00486; HMM-score: 25.8)
  HTH_34; Winged helix DNA-binding domain (PF13601; HMM-score: 19.7)
  HTH_36; Helix-turn-helix domain (PF13730; HMM-score: 14.4)
  MAGE; MAGE homology domain (PF01454; HMM-score: 11.9)
  no clan defined UPF0515; Uncharacterised protein UPF0515 (PF15135; HMM-score: 11.8)
  PsbQ-like (CL0752) CYP38_PsbQ-like; Peptidyl-prolyl cis-trans isomerase CYP38-like, PsbQ-like domain (PF21329; HMM-score: 9.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.8978
  • Cytoplasmic Membrane Score: 0.0715
  • Cell wall & surface Score: 0.0053
  • Extracellular Score: 0.0254
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.00274
  • TAT(Tat/SPI): 0.000127
  • LIPO(Sec/SPII): 0.000732
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151220268 NCBI
• RefSeq: YP_001331091 NCBI
• UniProt: A6QD96 UniProt

⊟Protein sequence

• MHIQGDKTMKYNNHDKIRDFIIIEAYMFRFKKKVKPEVDMTIKEFILLTYLFHQQENTLPFKKIVSDLCYKQSDLVQHIKVLVKHSYISKVRSKIDERNTYISISEEQREKIAERVTLFDQIIKQFNLADQSESQMIPKDSKEFLNLMMYTMYFKNIIKKHLTLSFVEFTILAIITSQNKNIVLLKDLIETIHHKYPQTVRALNNLKKQGYLIKERSTEDERKILIHMDDAQQDHAEQLLAQVNQLLADKDHLHLVFE

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications