PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_002385
• pan locus tag^?: SAUPAN005948000
• symbol: JSNZ_002385
• pan gene symbol^?: tcyA
• synonym:
• product: transporter substrate-binding domain-containing protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_002385
• symbol: JSNZ_002385
• product: transporter substrate-binding domain-containing protein
• replicon: chromosome
• strand: -
• coordinates: 2382048..2382827
• length: 780
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  
  ATGAAAAGACTTTTATTTGTGATGATAGCTTTCGTTTTCATATTGGCTGCATGTGGAAAC
  AATTCGTCGAAAGACAAGGAAGCTAGTAAAGATAGCAAAACAATTAATGTTGGTACTGAG
  GGGACTTATGCACCATTTAGTTTCCATGATAAAGATGGTAAATTAACTGGTTACGATATT
  GATGTTATTAAAGCAGTGGCTAAAGAAGAAGGTTTAAAACTTAAATTTAATGAAACTTCT
  TGGGATTCTATGTTTGCAGGTTTAGACGCGGGGCGTTTTGATGTAATCGCGAACCAAGTG
  GGTATAAATCCTGATAGAGAAAAGAAATATAAATTTTCTAAGCCTTACACATTCTCAAGT
  GCTGTTTTAGTTATTCGTGAAAATGAAAAAGATATTAAAGATTTTGATGATGTTAAAGGT
  AAGAAGTTAGCACAAACATTCACATCTAATTATGGTAAATTAGCTAAGGATAAAGGTGCT
  GATATTACAAAAGTTGATGGCTTTAACCAATCAATGGATTTATTATTGTCTAAGCGTGTT
  GATGGTACATTTAATGATAGTCTGTCATACTTGGATTATAAAAAACAAAAACCTAATGCT
  AAGATCAAAGCAATCAAAGGTAATGCTGAACAAAGTAGATCTGCATTTGCATTTTCTAAA
  AAAGCAGATGATGAAACAGTTCAAAAATTCAATGATGGCTTGAAAAAAATCGAGGAAAAC
  GGTGAATTAGCTAAAATAGGTAAGAAATGGTTTGGTCAAGATGTTTCTAAATCTAAATAG
  60
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  420
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  540
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  780
  

⊟Protein

⊟General

• locus tag: JSNZ_002385
• symbol: JSNZ_002385
• description: transporter substrate-binding domain-containing protein
• length: 259
• theoretical pI: 9.8833
• theoretical MW: 28903.7
• GRAVY: -0.619691

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Amino acids, peptides and amines lysine-arginine-ornithine-binding periplasmic protein (TIGR01096; HMM-score: 133.7)
  and 5 more
  Metabolism Transport and binding proteins Amino acids, peptides and amines ectoine/hydroxyectoine ABC transporter solute-binding protein EhuB (TIGR02995; HMM-score: 81.6)
  extracellular substrate-binding orphan protein, GRRM family (TIGR04262; HMM-score: 20)
  Metabolism Energy metabolism Other methanol oxidation system protein MoxJ (TIGR03870; HMM-score: 19.9)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids methanol oxidation system protein MoxJ (TIGR03870; HMM-score: 19.9)
  Metabolism Transport and binding proteins Amino acids, peptides and amines lipoprotein, PulS/OutS family (TIGR01004; HMM-score: 11.8)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  PBP (CL0177) SBP_bac_3; Bacterial extracellular solute-binding proteins, family 3 (PF00497; HMM-score: 146.7)
  and 5 more
  Lig_chan-Glu_bd; Ligated ion channel L-glutamate- and glycine-binding site (PF10613; HMM-score: 24.2)
  Phosphonate-bd; ABC transporter, phosphonate, periplasmic substrate-binding protein (PF12974; HMM-score: 24.2)
  no clan defined InPase; Inorganic pyrophosphatase (PF18823; HMM-score: 17.7)
  PBP (CL0177) NMT1; NMT1/THI5 like (PF09084; HMM-score: 14.6)
  SASP (CL0755) SspK; Small acid-soluble spore protein K family (PF08176; HMM-score: 13.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 3.33
  • Cellwall Score: 3.33
  • Extracellular Score: 3.33
  • Internal Helix: 1
• DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.0001
  • Cytoplasmic Membrane Score: 0.9758
  • Cell wall & surface Score: 0.0086
  • Extracellular Score: 0.0155
• LocateP:
• SignalP: Signal peptide LIPO(Sec/SPII) length 17 aa
  • SP(Sec/SPI): 0.000389
  • TAT(Tat/SPI): 0.000032
  • LIPO(Sec/SPII): 0.999467
  • Cleavage Site: CS pos: 17-18. LAA-CG. Pr: 0.9999
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MKRLLFVMIAFVFILAACGNNSSKDKEASKDSKTINVGTEGTYAPFSFHDKDGKLTGYDIDVIKAVAKEEGLKLKFNETSWDSMFAGLDAGRFDVIANQVGINPDREKKYKFSKPYTFSSAVLVIRENEKDIKDFDDVKGKKLAQTFTSNYGKLAKDKGADITKVDGFNQSMDLLLSKRVDGTFNDSLSYLDYKKQKPNAKIKAIKGNAEQSRSAFAFSKKADDETVQKFNDGLKKIEENGELAKIGKKWFGQDVSKSK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for N315

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications