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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_002301
  • pan locus tag?: SAUPAN005822000
  • symbol: hutG
  • pan gene symbol?: hutG
  • synonym:
  • product: formimidoylglutamase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_002301
  • symbol: hutG
  • product: formimidoylglutamase
  • replicon: chromosome
  • strand: -
  • coordinates: 2299078..2300013
  • length: 936
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    GTGTACAAGCAAGGTGAACCAAATTTATGGACTGGAAGGTTAGATAGTGAAACAGACCCG
    AAAAAATTTAGACATTTTCAAACAGTAACATTTGAAGATTTGTCTAAGCTGGAAAAGAGT
    AGTATGCCATCAGGGGTCGGTATATTAGGCTATGCTGTTGACAAAGGTGTTGCTTTAAAC
    AAGGGGCGCATTGGTGCAAAAGAAGGACCAGATGCGATTAAACAAGCATTTGCAGGTTTG
    CCGGATTTGAATCAATGTGAAACTTTAGTCGATTACGGAAATGTTTATCATGATCATGAG
    GAATTAATTGATACTCAAAAAGAATTTGCTATGCTTGCAGCGAAGTCAATTGCTAATCAT
    AGACAAACATTTTTATTAGGTGGTGGACATGATATTGCGTATGCTCAATATTTAGCAACA
    CGTAAAGTCTATCCAACACAATCTATTGGTGTGATAAATATTGATGCGCATTTTGATACA
    CGTGCTGAACAACAATCTACATCTGGAACGAGCTTTAGACAAATTTTAGAAGAAGATGAA
    AACACAGATTATTTAGTGCTTGGTATTGCTCAAGGTGGTAATACGCAAAGTTTATTTGAT
    TATGCTAAAGAGAAAAAGATTGATTATGTCTTTGCAGATGAATTATTGAGTCACGTATCA
    CCAACAATTAAAGATATGATTGAACGTTTTGTACATGAACATGATGTCATTATGTTTACG
    ATTTGTATGGATGTCATTGATAGTGCGTTTGCGCCTGGGGTAAGTGCGCCGGCAGTGTTA
    GGTTTGTATCCACATACTGTTCTTGAATTAGCAAAACGTATTATTCCAAGTGATAAGGTG
    TCTTCAGTTAGTATTGCTGAGATGAATCCAACATATGACGCTGACAATAGAACTGCTAAG
    CTCGTTGCTAATTTAGTGCATCATTTTTTAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    936

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_002301
  • symbol: HutG
  • description: formimidoylglutamase
  • length: 311
  • theoretical pI: 5.35965
  • theoretical MW: 34512.8
  • GRAVY: -0.262379

⊟Function[edit | edit source]

  • reaction:
    EC 3.5.3.8?  ExPASy
    Formimidoylglutamase N-formimidoyl-L-glutamate + H2O = L-glutamate + formamide
  • TIGRFAM:
    Metabolism Energy metabolism Amino acids and amines formimidoylglutamase (TIGR01227; EC 3.5.3.8; HMM-score: 442.6)
    and 3 more
    agmatinase (TIGR01230; EC 3.5.3.11; HMM-score: 83.5)
    arginase (TIGR01229; EC 3.5.3.1; HMM-score: 62)
    pseudaminic acid biosynthesis-associated methylase (TIGR03587; HMM-score: 13.7)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    Arginase (CL0302) Arginase; Arginase family (PF00491; HMM-score: 164.5)
    and 1 more
    NADP_Rossmann (CL0063) CoA_binding_3; CoA-binding domain (PF13727; HMM-score: 12.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9755
    • Cytoplasmic Membrane Score: 0.0169
    • Cell wall & surface Score: 0.0006
    • Extracellular Score: 0.007
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006721
    • TAT(Tat/SPI): 0.004175
    • LIPO(Sec/SPII): 0.000839
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MYKQGEPNLWTGRLDSETDPKKFRHFQTVTFEDLSKLEKSSMPSGVGILGYAVDKGVALNKGRIGAKEGPDAIKQAFAGLPDLNQCETLVDYGNVYHDHEELIDTQKEFAMLAAKSIANHRQTFLLGGGHDIAYAQYLATRKVYPTQSIGVINIDAHFDTRAEQQSTSGTSFRQILEEDENTDYLVLGIAQGGNTQSLFDYAKEKKIDYVFADELLSHVSPTIKDMIERFVHEHDVIMFTICMDVIDSAFAPGVSAPAVLGLYPHTVLELAKRIIPSDKVSSVSIAEMNPTYDADNRTAKLVANLVHHFLK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: LacR* (repression) regulon, SigB (activation) regulon
    LacR*(TF)important in Lactose utilization;  regulation predicted or transferred from N315 and NCTC 8325  [1]
    SigB(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation;  regulation predicted or transferred from N315 and NCTC 8325  [2] [3] [4]   other strains

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)
  2. ↑ Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J Bacteriol: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)
  3. ↑ Jan Pané-Farré, Beate Jonas, Konrad Förstner, Susanne Engelmann, Michael Hecker
    The sigmaB regulon in Staphylococcus aureus and its regulation.
    Int J Med Microbiol: 2006, 296(4-5);237-58
    [PubMed:16644280] [WorldCat.org] [DOI] (P p)
  4. ↑ Bettina Schulthess, Dominik A Bloes, Patrice François, Myriam Girard, Jacques Schrenzel, Markus Bischoff, Brigitte Berger-Bächi
    The σB-dependent yabJ-spoVG operon is involved in the regulation of extracellular nuclease, lipase, and protease expression in Staphylococcus aureus.
    J Bacteriol: 2011, 193(18);4954-62
    [PubMed:21725011] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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