PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_002228
• pan locus tag^?: SAUPAN005718000
• symbol: femX
• pan gene symbol^?: femX
• synonym:
• product: lipid II:glycine glycyltransferase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_002228
• symbol: femX
• product: lipid II:glycine glycyltransferase
• replicon: chromosome
• strand: -
• coordinates: 2230333..2231598
• length: 1266
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  
  ATGGAAAAGATGCATATCACTAATCAGGAACATGACGCATTTGTTAAATCTCACCCAAAT
  GGAGATTTATTACAATTAACGAAATGGGCAGAAACAAAGAAATTAACTGGATGGTACGCG
  CGAAGAATCGCTGTAGGTCGTGACGGTGAAGTTCAGGGTGTTGCGCAATTACTTTTTAAA
  AAAGTACCTAAATTACCGTATACGCTATGTTATATTTCACGTGGTTTTGTTGTTGATTAT
  AGTAATAAAGAAGCGTTAAATGCATTGTTAGACAGTGCAAAAGAAATTGCTAAAGCTGAG
  AAAGCGTATGCAATTAAAATCGATCCTGATGTTGAAGTTGATAAAGGTACAGATGCTTTG
  CAAAATTTGAAAGCGCTTGGTTTTAAACATAAAGGATTTAAAGAAGGTTTATCAAAAGAC
  TACATCCAACCACGTATGACTATGATTACACCAATTGATAAAAATGATGATGAGTTATTA
  AATAGTTTTGAACGCCGAAATCGTTCAAAAGTGCGCTTGGCTTTAAAGCGAGGTACGACA
  GTAGAACGATCTGATAGAGAAGGTTTAAAAACATTTGCTGAATTAATGAAAATCACTGGG
  GAACGCGATGGCTTCTTAACGCGTGATATTAGTTACTTTGAAAATATTTATGATGCGTTG
  CATGAAGATGGAGATGCTGAACTATTTTTAGTAAAGTTGGACCCAAAAGAAAATATAGCG
  AAAGTAAATCAAGAATTGAATGAACTTCATGCCGAAATAGCTAAATGGCAGCAGAAGATG
  GAAACATCTGAAAAGCAAGCTAAAAAAGCGCAAAATATGATTAATGATGCGCAAAATAAA
  ATTGCTAAAAATGAAGATTTAAAACGAGACCTAGAAGCTTTAGAAAAGGAACATCCTGAA
  GGTATTTATCTTTCTGGTGCACTATTAATGTTTGCTGGCTCAAAATCATATTACTTATAT
  GGTGCGTCTTCTAATGAATTTAGAGATTTTTTACCAAATCACCATATGCAGTATACGATG
  ATGAAGTATGCACGTGAACATGGTGCAACAACTTACGATTTCGGTGGTACAGATAATGAT
  CCAGATAAAGACTCTGAACATTATGGCTTATGGGCATTTAAAAAAGTGTGGGGAACATAC
  TTAAGTGAAAAGATTGGTGAATTTGATTATATATTGAATCAGCCATTGTACCAATTAATT
  GAGCAAGTTAAACCGCGTTTAACAAAAGCTAAAATTAAAATATCTCGTAAATTAAAACGA
  AAATAG
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1266
  

⊟Protein

⊟General

• locus tag: JSNZ_002228
• symbol: FemX
• description: lipid II:glycine glycyltransferase
• length: 421
• theoretical pI: 9.06529
• theoretical MW: 48536
• GRAVY: -0.72114

⊟Function

• reaction:
  EC 2.3.2.16?  ExPASy
  Lipid II:glycine glycyltransferase MurNAc-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc + glycyl-tRNA(Gly) = MurNAc-L-Ala-D-isoglutaminyl-L-Lys-(N⁶-Gly)-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc + tRNA(Gly)
• TIGRFAM:
  Unknown function General FemAB-related protein, PEP-CTERM system-associated (TIGR03019; HMM-score: 57.9)
  and 3 more
  SH3 domain protein (TIGR04211; HMM-score: 17.3)
  two transmembrane protein (TIGR04527; HMM-score: 10.3)
  CRISPR type III-A/MTUBE-associated protein Csm2 (TIGR01870; HMM-score: 8.1)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  Acetyltrans (CL0257) FemAB; FemAB family (PF02388; HMM-score: 266.6)
  and 22 more
  Acetyltransf_6; Acetyltransferase (GNAT) domain (PF13480; HMM-score: 47.1)
  Concanavalin (CL0004) Laminin_II; Laminin Domain II (PF06009; HMM-score: 17.2)
  P-loop_NTPase (CL0023) AAA_13; AAA domain (PF13166; HMM-score: 16.6)
  PDDEXK (CL0236) RmuC; RmuC family (PF02646; HMM-score: 16.1)
  FtsL (CL0225) DivIC; Septum formation initiator (PF04977; HMM-score: 15.4)
  no clan defined ACBP4_C; Acyl-CoA-binding domain-containing protein 4-like, C-terminal (PF24922; HMM-score: 14.8)
  TMF_TATA_bd; TATA element modulatory factor 1 TATA binding (PF12325; HMM-score: 14.6)
  HAUS-augmin3; HAUS augmin-like complex subunit 3 (PF14932; HMM-score: 13.5)
  Cep57_CLD_2; Centrosome localisation domain of PPC89 (PF14197; HMM-score: 13.4)
  DUF6586; Family of unknown function (DUF6586) (PF20227; HMM-score: 13.4)
  DUF6247; Family of unknown function (DUF6247) (PF19760; HMM-score: 13.2)
  MG280; Uncharacterised protein MG280 (PF23067; HMM-score: 12.7)
  Pec_lyase-like (CL0268) FapA; Flagellar Assembly Protein A beta solenoid domain (PF03961; HMM-score: 12.4)
  no clan defined TMPIT; TMPIT-like protein (PF07851; HMM-score: 11.9)
  Ax_dynein_light; Axonemal dynein light chain (PF10211; HMM-score: 11.5)
  BCLiA (CL0551) ATG14; Vacuolar sorting 38 and autophagy-related subunit 14 (PF10186; HMM-score: 10.9)
  no clan defined BLI1; BLOC-1 interactor 1 (PF17324; HMM-score: 9.7)
  PCRF; PCRF domain (PF03462; HMM-score: 9.2)
  VBS-like (CL0705) HIP1_clath_bdg; Clathrin-binding domain of Huntingtin-interacting protein 1 (PF16515; HMM-score: 8.6)
  no clan defined BRE1; BRE1 E3 ubiquitin ligase (PF08647; HMM-score: 8)
  DUF3987; Protein of unknown function (DUF3987) (PF13148; HMM-score: 7.2)
  UBC (CL0208) Knl1_RWD_C; Knl1 RWD C-terminal domain (PF18210; HMM-score: 6.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.8456
  • Cytoplasmic Membrane Score: 0.1191
  • Cell wall & surface Score: 0.003
  • Extracellular Score: 0.0323
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.007329
  • TAT(Tat/SPI): 0.000932
  • LIPO(Sec/SPII): 0.000722
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MEKMHITNQEHDAFVKSHPNGDLLQLTKWAETKKLTGWYARRIAVGRDGEVQGVAQLLFKKVPKLPYTLCYISRGFVVDYSNKEALNALLDSAKEIAKAEKAYAIKIDPDVEVDKGTDALQNLKALGFKHKGFKEGLSKDYIQPRMTMITPIDKNDDELLNSFERRNRSKVRLALKRGTTVERSDREGLKTFAELMKITGERDGFLTRDISYFENIYDALHEDGDAELFLVKLDPKENIAKVNQELNELHAEIAKWQQKMETSEKQAKKAQNMINDAQNKIAKNEDLKRDLEALEKEHPEGIYLSGALLMFAGSKSYYLYGASSNEFRDFLPNHHMQYTMMKYAREHGATTYDFGGTDNDPDKDSEHYGLWAFKKVWGTYLSEKIGEFDYILNQPLYQLIEQVKPRLTKAKIKISRKLKRK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications