PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_002178
• pan locus tag^?: SAUPAN005625000
• symbol: alsS
• pan gene symbol^?: budB
• synonym:
• product: acetolactate synthase AlsS

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_002178
• symbol: alsS
• product: acetolactate synthase AlsS
• replicon: chromosome
• strand: -
• coordinates: 2194615..2196279
• length: 1665
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  1501
  1561
  1621
  
  ATGACTGATAAAAAGTACACTGCAGCCGATATGGTTATTGATACTTTGAAAAATAATGGG
  GTAGAATATGTTTTTGGTATTCCGGGTGCAAAGATTGACTATCTATTTAATGCTTTAATT
  GATGATGGTCCTGAACTTATTGTCACTCGTCATGAACAAAACGCCGCAATGATGGCGCAA
  GGTATTGGAAGATTAACAGGCAAACCGGGTGTAGTACTTGTTACAAGTGGTCCTGGTGTA
  AGTAATTTAACTACTGGATTATTAACCGCAACATCTGAAGGGGATCCTGTATTAGCGTTA
  GGTGGCCAAGTGAAACGTAATGATTTATTACGATTAACGCATCAAAGTATTGATAATGCT
  GCGCTATTAAAATATTCATCAAAATATAGTGAAGAAGTACAAGATCCTGAATCATTATCA
  GAAGTTATGACAAATGCAATTCGAATTGCTACTTCAGGAAAAAATGGCGCAAGTTTTATT
  AGTATTCCGCAAGATGTGATTTCTTCACCAGTTGAATCTAAGGCGATATCACTTTGTCAA
  AAACCAAATTTAGGTGTACCAAGTGAACAAGATATTAATGATGTCATTGAAGCGATTAAA
  AATGCATCATTTCCGGTTTTATTAGCTGGAATGAGAAGTTCAAGTGCAGACGAAACGAAT
  GCTATTCGCAAATTAGTAGAGCGTACCAATTTACCCGTTGTAGAAACATTCCAAGGTGCG
  GGTGTGATTAGTCGTGAATTAGAAAATCATTTCTTTGGGCGAGTGGGTTTATTCCGTAAT
  CAAGTTGGTGATGAATTATTACGTAAAAGTGATTTAGTTGTTACAATCGGTTATGATCCT
  ATAGAATATGAGGCTAGTAACTGGAATAAAGAATTAGATACGCAAATAATCAATATTGAC
  GAAGTTCAAGCTGAAATTACTAATTATATGCAACCGAAAAAAGAGTTGATTGGTAATATT
  GCTAAAACGATTGAAATGATTTCTGACAAAGTAGATGAACCATTTATAAATCAACAACAT
  TTGGATGAATTAGAAAAGTTAAGAGCGCATATTGATCAAGAAACTGGTATTAAAGCGACG
  CATGAAGAAGGCATCTTACACCCAGTTGAAATTATAGAATCTATGCAAAAGGTATTAACT
  GATGAAACAACAGTGACAGTTGATGTTGGTAGTCACTATATTTGGATGGCACGTAATTTC
  AGAAGTTACAATCCAAGACATTTATTATTTAGTAACGGTATGCAAACGCTTGGTGTAGCA
  TTACCGTGGGCAATTTCAGCTGCGCTTGTACGTCCTAATACGCAAGTTGTATCCGTTGCT
  GGCGATGGAGGCTTTTTATTTTCATCACAAGATTTAGAAACAGCCGTACGTAAAAATCTA
  AACATTATTCAACTTATTTGGAACGATGGAAAATATAACATGGTTGAATTCCAAGAAGAA
  ATGAAATATAAACGTTCATCAGGCGTAGACTTCGGTCCGGTAGATTTCGTAAAATATGCT
  GAATCATTTGGCGCAAAAGGTTTACGAGTTACTAATCAAGAAGAATTAGAAGCGGCAATT
  AAAGAGGGGTATGAAACAGATGGTCCAGTACTAATTGATATACCTGTAAATTACAAAGAT
  AATATCAAACTTTCAACAAATATGTTACCTGACGTATTTAACTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1380
  1440
  1500
  1560
  1620
  1665
  

⊟Protein

⊟General

• locus tag: JSNZ_002178
• symbol: AlsS
• description: acetolactate synthase AlsS
• length: 554
• theoretical pI: 4.5655
• theoretical MW: 61109.8
• GRAVY: -0.218592

⊟Function

• reaction:
  EC 2.2.1.6?  ExPASy
  Acetolactate synthase 2 pyruvate = 2-acetolactate + CO₂
• TIGRFAM:
  Metabolism Energy metabolism Fermentation acetolactate synthase, catabolic (TIGR02418; EC 2.2.1.6; HMM-score: 929.6)
  and 15 more
  Metabolism Amino acid biosynthesis Pyruvate family acetolactate synthase, large subunit, biosynthetic type (TIGR00118; EC 2.2.1.6; HMM-score: 345)
  Metabolism Energy metabolism Aerobic pyruvate oxidase (TIGR02720; EC 1.2.3.3; HMM-score: 240.6)
  Cellular processes Cellular processes Detoxification oxalyl-CoA decarboxylase (TIGR03254; EC 4.1.1.8; HMM-score: 198.1)
  Metabolism Central intermediary metabolism Other sulfoacetaldehyde acetyltransferase (TIGR03457; EC 2.3.3.15; HMM-score: 187.9)
  glyoxylate carboligase (TIGR01504; EC 4.1.1.47; HMM-score: 171.7)
  Metabolism Energy metabolism Sugars 3,5/4-trihydroxycyclohexa-1,2-dione hydrolase (TIGR04377; EC 3.7.1.-; HMM-score: 161.1)
  indolepyruvate/phenylpyruvate decarboxylase (TIGR03394; EC 4.1.1.43,4.1.1.74; HMM-score: 94.1)
  Metabolism Central intermediary metabolism Other indolepyruvate decarboxylase (TIGR03393; EC 4.1.1.74; HMM-score: 60.5)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase (TIGR00173; EC 2.2.1.9; HMM-score: 56.9)
  phosphonopyruvate decarboxylase (TIGR03297; EC 4.1.1.82; HMM-score: 25.6)
  sulfopyruvate decarboxylase, beta subunit (TIGR03846; EC 4.1.1.79; HMM-score: 24.8)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other sulfopyruvate decarboxylase, alpha subunit (TIGR03845; EC 4.1.1.79; HMM-score: 21.1)
  Metabolism Energy metabolism Methanogenesis sulfopyruvate decarboxylase, alpha subunit (TIGR03845; EC 4.1.1.79; HMM-score: 21.1)
  2-oxoacid:acceptor oxidoreductase, beta subunit, pyruvate/2-ketoisovalerate family (TIGR02177; HMM-score: 19.2)
  Metabolism Energy metabolism Chemoautotrophy CO dehydrogenase/acetyl-CoA synthase complex, epsilon subunit (TIGR00315; EC 1.2.99.2; HMM-score: 15.6)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  THDP-binding (CL0254) TPP_enzyme_C; Thiamine pyrophosphate enzyme, C-terminal TPP binding domain (PF02775; HMM-score: 161.4)
  and 3 more
  TPP_enzyme_N; Thiamine pyrophosphate enzyme, N-terminal TPP binding domain (PF02776; HMM-score: 125.7)
  NADP_Rossmann (CL0063) TPP_enzyme_M; Thiamine pyrophosphate enzyme, central domain (PF00205; HMM-score: 82.6)
  P-loop_NTPase (CL0023) AAA_23; AAA domain (PF13476; HMM-score: 13.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 1.05
  • Cytoplasmic Membrane Score: 8.78
  • Cellwall Score: 0.08
  • Extracellular Score: 0.09
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9111
  • Cytoplasmic Membrane Score: 0.0764
  • Cell wall & surface Score: 0.0003
  • Extracellular Score: 0.0123
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004002
  • TAT(Tat/SPI): 0.000185
  • LIPO(Sec/SPII): 0.000309
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MTDKKYTAADMVIDTLKNNGVEYVFGIPGAKIDYLFNALIDDGPELIVTRHEQNAAMMAQGIGRLTGKPGVVLVTSGPGVSNLTTGLLTATSEGDPVLALGGQVKRNDLLRLTHQSIDNAALLKYSSKYSEEVQDPESLSEVMTNAIRIATSGKNGASFISIPQDVISSPVESKAISLCQKPNLGVPSEQDINDVIEAIKNASFPVLLAGMRSSSADETNAIRKLVERTNLPVVETFQGAGVISRELENHFFGRVGLFRNQVGDELLRKSDLVVTIGYDPIEYEASNWNKELDTQIINIDEVQAEITNYMQPKKELIGNIAKTIEMISDKVDEPFINQQHLDELEKLRAHIDQETGIKATHEEGILHPVEIIESMQKVLTDETTVTVDVGSHYIWMARNFRSYNPRHLLFSNGMQTLGVALPWAISAALVRPNTQVVSVAGDGGFLFSSQDLETAVRKNLNIIQLIWNDGKYNMVEFQEEMKYKRSSGVDFGPVDFVKYAESFGAKGLRVTNQEELEAAIKEGYETDGPVLIDIPVNYKDNIKLSTNMLPDVFN

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications