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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_002178
  • pan locus tag?: SAUPAN005625000
  • symbol: alsS
  • pan gene symbol?: budB
  • synonym:
  • product: acetolactate synthase AlsS

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_002178
  • symbol: alsS
  • product: acetolactate synthase AlsS
  • replicon: chromosome
  • strand: -
  • coordinates: 2194615..2196279
  • length: 1665
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGACTGATAAAAAGTACACTGCAGCCGATATGGTTATTGATACTTTGAAAAATAATGGG
    GTAGAATATGTTTTTGGTATTCCGGGTGCAAAGATTGACTATCTATTTAATGCTTTAATT
    GATGATGGTCCTGAACTTATTGTCACTCGTCATGAACAAAACGCCGCAATGATGGCGCAA
    GGTATTGGAAGATTAACAGGCAAACCGGGTGTAGTACTTGTTACAAGTGGTCCTGGTGTA
    AGTAATTTAACTACTGGATTATTAACCGCAACATCTGAAGGGGATCCTGTATTAGCGTTA
    GGTGGCCAAGTGAAACGTAATGATTTATTACGATTAACGCATCAAAGTATTGATAATGCT
    GCGCTATTAAAATATTCATCAAAATATAGTGAAGAAGTACAAGATCCTGAATCATTATCA
    GAAGTTATGACAAATGCAATTCGAATTGCTACTTCAGGAAAAAATGGCGCAAGTTTTATT
    AGTATTCCGCAAGATGTGATTTCTTCACCAGTTGAATCTAAGGCGATATCACTTTGTCAA
    AAACCAAATTTAGGTGTACCAAGTGAACAAGATATTAATGATGTCATTGAAGCGATTAAA
    AATGCATCATTTCCGGTTTTATTAGCTGGAATGAGAAGTTCAAGTGCAGACGAAACGAAT
    GCTATTCGCAAATTAGTAGAGCGTACCAATTTACCCGTTGTAGAAACATTCCAAGGTGCG
    GGTGTGATTAGTCGTGAATTAGAAAATCATTTCTTTGGGCGAGTGGGTTTATTCCGTAAT
    CAAGTTGGTGATGAATTATTACGTAAAAGTGATTTAGTTGTTACAATCGGTTATGATCCT
    ATAGAATATGAGGCTAGTAACTGGAATAAAGAATTAGATACGCAAATAATCAATATTGAC
    GAAGTTCAAGCTGAAATTACTAATTATATGCAACCGAAAAAAGAGTTGATTGGTAATATT
    GCTAAAACGATTGAAATGATTTCTGACAAAGTAGATGAACCATTTATAAATCAACAACAT
    TTGGATGAATTAGAAAAGTTAAGAGCGCATATTGATCAAGAAACTGGTATTAAAGCGACG
    CATGAAGAAGGCATCTTACACCCAGTTGAAATTATAGAATCTATGCAAAAGGTATTAACT
    GATGAAACAACAGTGACAGTTGATGTTGGTAGTCACTATATTTGGATGGCACGTAATTTC
    AGAAGTTACAATCCAAGACATTTATTATTTAGTAACGGTATGCAAACGCTTGGTGTAGCA
    TTACCGTGGGCAATTTCAGCTGCGCTTGTACGTCCTAATACGCAAGTTGTATCCGTTGCT
    GGCGATGGAGGCTTTTTATTTTCATCACAAGATTTAGAAACAGCCGTACGTAAAAATCTA
    AACATTATTCAACTTATTTGGAACGATGGAAAATATAACATGGTTGAATTCCAAGAAGAA
    ATGAAATATAAACGTTCATCAGGCGTAGACTTCGGTCCGGTAGATTTCGTAAAATATGCT
    GAATCATTTGGCGCAAAAGGTTTACGAGTTACTAATCAAGAAGAATTAGAAGCGGCAATT
    AAAGAGGGGTATGAAACAGATGGTCCAGTACTAATTGATATACCTGTAAATTACAAAGAT
    AATATCAAACTTTCAACAAATATGTTACCTGACGTATTTAACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1665

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_002178
  • symbol: AlsS
  • description: acetolactate synthase AlsS
  • length: 554
  • theoretical pI: 4.5655
  • theoretical MW: 61109.8
  • GRAVY: -0.218592

⊟Function[edit | edit source]

  • reaction:
    EC 2.2.1.6?  ExPASy
    Acetolactate synthase 2 pyruvate = 2-acetolactate + CO2
  • TIGRFAM:
    Metabolism Energy metabolism Fermentation acetolactate synthase, catabolic (TIGR02418; EC 2.2.1.6; HMM-score: 929.6)
    and 15 more
    Metabolism Amino acid biosynthesis Pyruvate family acetolactate synthase, large subunit, biosynthetic type (TIGR00118; EC 2.2.1.6; HMM-score: 345)
    Metabolism Energy metabolism Aerobic pyruvate oxidase (TIGR02720; EC 1.2.3.3; HMM-score: 240.6)
    Cellular processes Cellular processes Detoxification oxalyl-CoA decarboxylase (TIGR03254; EC 4.1.1.8; HMM-score: 198.1)
    Metabolism Central intermediary metabolism Other sulfoacetaldehyde acetyltransferase (TIGR03457; EC 2.3.3.15; HMM-score: 187.9)
    glyoxylate carboligase (TIGR01504; EC 4.1.1.47; HMM-score: 171.7)
    Metabolism Energy metabolism Sugars 3,5/4-trihydroxycyclohexa-1,2-dione hydrolase (TIGR04377; EC 3.7.1.-; HMM-score: 161.1)
    indolepyruvate/phenylpyruvate decarboxylase (TIGR03394; EC 4.1.1.43,4.1.1.74; HMM-score: 94.1)
    Metabolism Central intermediary metabolism Other indolepyruvate decarboxylase (TIGR03393; EC 4.1.1.74; HMM-score: 60.5)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase (TIGR00173; EC 2.2.1.9; HMM-score: 56.9)
    phosphonopyruvate decarboxylase (TIGR03297; EC 4.1.1.82; HMM-score: 25.6)
    sulfopyruvate decarboxylase, beta subunit (TIGR03846; EC 4.1.1.79; HMM-score: 24.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other sulfopyruvate decarboxylase, alpha subunit (TIGR03845; EC 4.1.1.79; HMM-score: 21.1)
    Metabolism Energy metabolism Methanogenesis sulfopyruvate decarboxylase, alpha subunit (TIGR03845; EC 4.1.1.79; HMM-score: 21.1)
    2-oxoacid:acceptor oxidoreductase, beta subunit, pyruvate/2-ketoisovalerate family (TIGR02177; HMM-score: 19.2)
    Metabolism Energy metabolism Chemoautotrophy CO dehydrogenase/acetyl-CoA synthase complex, epsilon subunit (TIGR00315; EC 1.2.99.2; HMM-score: 15.6)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    THDP-binding (CL0254) TPP_enzyme_C; Thiamine pyrophosphate enzyme, C-terminal TPP binding domain (PF02775; HMM-score: 161.4)
    and 3 more
    TPP_enzyme_N; Thiamine pyrophosphate enzyme, N-terminal TPP binding domain (PF02776; HMM-score: 125.7)
    NADP_Rossmann (CL0063) TPP_enzyme_M; Thiamine pyrophosphate enzyme, central domain (PF00205; HMM-score: 82.6)
    P-loop_NTPase (CL0023) AAA_23; AAA domain (PF13476; HMM-score: 13.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 1.05
    • Cytoplasmic Membrane Score: 8.78
    • Cellwall Score: 0.08
    • Extracellular Score: 0.09
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9111
    • Cytoplasmic Membrane Score: 0.0764
    • Cell wall & surface Score: 0.0003
    • Extracellular Score: 0.0123
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004002
    • TAT(Tat/SPI): 0.000185
    • LIPO(Sec/SPII): 0.000309
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MTDKKYTAADMVIDTLKNNGVEYVFGIPGAKIDYLFNALIDDGPELIVTRHEQNAAMMAQGIGRLTGKPGVVLVTSGPGVSNLTTGLLTATSEGDPVLALGGQVKRNDLLRLTHQSIDNAALLKYSSKYSEEVQDPESLSEVMTNAIRIATSGKNGASFISIPQDVISSPVESKAISLCQKPNLGVPSEQDINDVIEAIKNASFPVLLAGMRSSSADETNAIRKLVERTNLPVVETFQGAGVISRELENHFFGRVGLFRNQVGDELLRKSDLVVTIGYDPIEYEASNWNKELDTQIINIDEVQAEITNYMQPKKELIGNIAKTIEMISDKVDEPFINQQHLDELEKLRAHIDQETGIKATHEEGILHPVEIIESMQKVLTDETTVTVDVGSHYIWMARNFRSYNPRHLLFSNGMQTLGVALPWAISAALVRPNTQVVSVAGDGGFLFSSQDLETAVRKNLNIIQLIWNDGKYNMVEFQEEMKYKRSSGVDFGPVDFVKYAESFGAKGLRVTNQEELEAAIKEGYETDGPVLIDIPVNYKDNIKLSTNMLPDVFN

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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