From AureoWiki
Jump to navigation Jump to search

FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_002146
  • pan locus tag?: SAUPAN005567000
  • symbol: JSNZ_002146
  • pan gene symbol?: htsA
  • synonym:
  • product: Fe(3+) dicitrate ABC transporter substrate-binding protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_002146
  • symbol: JSNZ_002146
  • product: Fe(3+) dicitrate ABC transporter substrate-binding protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2162753..2163736
  • length: 984
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAGAGGTCTAAAAACTTTTAGTATATTGGGATTAATAGTTGCCTTACTTTTAGTTGCA
    GGTTGTGGTAATACGGATAATTCAAGTAAAAAAGAATCATCAACTAAAGATACTATTTCG
    GTAAAAGATGAAAATGGTACAGTAAAAGTACCTAAAGATGCAAAACGTATCGTTGTATTA
    GAGTACTCATTTGCAGATGCATTAGCAGCATTAGACGTTAAACCAGTTGGTATTGCTGAT
    GATGGTAAGAAAAAACGTATCATTAAACCAGTTAGAGAAAAAATTGGGGATTATACTTCT
    GTAGGTACACGTAAACAGCCAAACTTAGAGGAAATTAGTAAATTAAAACCGGATTTAATT
    ATCGCTGATAGCAGTAGACATAAAGGTATTAATAAAGAATTAAACAAAATTGCACCAACA
    TTATCATTAAAGAGTTTTGATGGAGACTACAAACAAAATATTAATTCGTTCAAAACAATT
    GCTAAAGCTTTAAATAAAGAAAAAGAAGGCGAAAAACGTCTTGCTGAGCATGATAAATTA
    ATCAATAAGTATAAAGATGAAATTAAATTTGATAGAAATCAAAAAGTGCTTCCAGCAGTA
    GTTGCTAAAGCTGGTTTATTAGCACATCCAAACTATTCATATGTTGGACAATTTTTAAAC
    GAACTAGGATTTAAAAATGCATTAAGTGACGATGTAACAAAAGGTTTAAGTAAATATTTG
    AAAGGACCTTACTTACAATTAGACACTGAACATTTAGCTGATTTAAATCCAGAGCGTATG
    ATCATTATGACAGATCATGCTAAAAAAGATTCTGCTGAATTCAAGAAGTTACAAGAAGAT
    GCAACATGGAAAAAGTTGAATGCAGTTAAAAATAATCGCGTGGATATTGTTGACCGTGAT
    GTTTGGGCAAGATCTCGTGGCTTAATTTCTTCTGAAGAAATGGCTAAAGAACTTGTTGAA
    TTATCAAAAAAAGAACAAAAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    984

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_002146
  • symbol: JSNZ_002146
  • description: Fe(3+) dicitrate ABC transporter substrate-binding protein
  • length: 327
  • theoretical pI: 10.05
  • theoretical MW: 36576.9
  • GRAVY: -0.569113

⊟Function[edit | edit source]

  • TIGRFAM:
    heme ABC transporter, heme-binding protein isdE (TIGR03659; HMM-score: 95.6)
    and 2 more
    putative ABC transporter PGF-CTERM-modified substrate-binding protein (TIGR04281; HMM-score: 15.7)
    proposed F420-0 ABC transporter, periplasmic F420-0 binding protein (TIGR03868; HMM-score: 13.4)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    Chelatase (CL0043) Peripla_BP_2; Periplasmic binding protein (PF01497; HMM-score: 116.5)
    and 4 more
    no clan defined DUF6556; Family of unknown function (DUF6556) (PF20193; HMM-score: 15.3)
    OML_zippers (CL0590) FadA; Adhesion protein FadA (PF09403; HMM-score: 15.2)
    no clan defined CCDC66; Coiled-coil domain-containing protein 66 (PF15236; HMM-score: 14.1)
    LppaM (CL0421) LPAM_2; Prokaryotic lipoprotein-attachment site (PF13627; HMM-score: 10.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 9.68
    • Cellwall Score: 0.17
    • Extracellular Score: 0.16
    • Internal Helix: 1
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.9883
    • Cell wall & surface Score: 0.0044
    • Extracellular Score: 0.0073
  • LocateP:
  • SignalP: Signal peptide LIPO(Sec/SPII) length 21 aa
    • SP(Sec/SPI): 0.000436
    • TAT(Tat/SPI): 0.000177
    • LIPO(Sec/SPII): 0.999108
    • Cleavage Site: CS pos: 21-22. VAG-CG. Pr: 0.9998
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MRGLKTFSILGLIVALLLVAGCGNTDNSSKKESSTKDTISVKDENGTVKVPKDAKRIVVLEYSFADALAALDVKPVGIADDGKKKRIIKPVREKIGDYTSVGTRKQPNLEEISKLKPDLIIADSSRHKGINKELNKIAPTLSLKSFDGDYKQNINSFKTIAKALNKEKEGEKRLAEHDKLINKYKDEIKFDRNQKVLPAVVAKAGLLAHPNYSYVGQFLNELGFKNALSDDVTKGLSKYLKGPYLQLDTEHLADLNPERMIIMTDHAKKDSAEFKKLQEDATWKKLNAVKNNRVDIVDRDVWARSRGLISSEEMAKELVELSKKEQK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: Fur* (repression) regulon
    Fur*(TF)important in Iron homeostasis;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=JSNZ_002146&oldid=107787"