From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2167 [new locus tag: SACOL_RS11390 ]
  • pan locus tag?: SAUPAN005567000
  • symbol: SACOL2167
  • pan gene symbol?: htsA
  • synonym:
  • product: iron compound ABC transporter iron compound-binding protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2167 [new locus tag: SACOL_RS11390 ]
  • symbol: SACOL2167
  • product: iron compound ABC transporter iron compound-binding protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2245863..2246846
  • length: 984
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAGAGGTCTAAAAACTTTTAGTATATTGGGATTAATAGTTGCCTTACTTTTAGTTGCA
    GCTTGTGGTAATACGGATAATTCAAGTAAAAAAGAATCATCAACTAAAGATACTATTTCG
    GTAAAAGATGAAAATGGTACAGTAAAAGTACCTAAAGATGCAAAACGTATCGTTGTATTA
    GAGTACTCATTTGCAGATGCATTAGCAGCATTAGACGTTAAACCAGTTGGTATTGCTGAT
    GATGGTAAGAAAAAACGTATCATTAAACCAGTTAGAGAAAAAATTGGGGATTATACTTCT
    GTAGGTACACGTAAACAGCCAAACTTAGAAGAAATTAGTAAATTAAAACCGGATTTAATT
    ATCGCTGATAGCAGTAGACATAAAGGTATTAATAAAGAATTAAACAAAATTGCACCAACA
    TTATCATTAAAGAGTTTTGATGGAGACTACAAACAAAATATTAATTCGTTCAAAACAATT
    GCTAAAGCTTTAAATAAAGAAAAAGAAGGCGAAAAACGTCTTGCTGAGCATGATAAATTA
    ATCAATAAGTATAAAGATGAAATTAAATTTGATAGAAATCAAAAAGTGCTTCCAGCAGTA
    GTTGCTAAAGCTGGTTTATTAGCACATCCAAACTATTCATATGTTGGACAATTTTTAAAC
    GAACTAGGATTTAAAAATGCATTAAGTGACGATGTAACAAAAGGTTTAAGTAAATATTTG
    AAAGGACCTTACTTACAATTAGACACTGAACATTTAGCTGATTTAAATCCAGAGCGTATG
    ATCATTATGACAGATCATGCTAAAAAAGATTCTGCTGAATTCAAGAAGTTACAAGAAGAT
    GCAACATGGAAAAAGTTGAATGCAGTTAAAAATAATCGCGTGGATATTGTTGACCGTGAT
    GTTTGGGCAAGATCTCGTGGCTTAATTTCTTCTGAAGAAATGGCTAAAGAACTTGTTGAA
    TTATCAAAAAAAGAACAAAAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    984

⊟Protein[edit | edit source]

Protein Data Bank: 3EIW
Protein Data Bank: 3EIX
Protein Data Bank: 3LHS
Protein Data Bank: 3LI2

⊟General[edit | edit source]

  • locus tag: SACOL2167 [new locus tag: SACOL_RS11390 ]
  • symbol: SACOL2167
  • description: iron compound ABC transporter iron compound-binding protein
  • length: 327
  • theoretical pI: 10.05
  • theoretical MW: 36590.9
  • GRAVY: -0.562385

⊟Function[edit | edit source]

  • TIGRFAM:
    heme ABC transporter, heme-binding protein isdE (TIGR03659; HMM-score: 95)
    and 2 more
    putative ABC transporter PGF-CTERM-modified substrate-binding protein (TIGR04281; HMM-score: 15.7)
    proposed F420-0 ABC transporter, periplasmic F420-0 binding protein (TIGR03868; HMM-score: 13.5)
  • TheSEED  :
    • Ferric-staphyloferrin A ABC transporter, substrate-binding protein
    Iron acquisition and metabolism Iron acquisition and metabolism - no subcategory Heme, hemin uptake and utilization systems in GramPositives  Heme ABC type transporter HtsABC, heme-binding protein
    and 1 more
    Stress Response Stress Response - no subcategory Flavohaemoglobin  Heme ABC type transporter HtsABC, heme-binding protein
  • PFAM:
    Chelatase (CL0043) Peripla_BP_2; Periplasmic binding protein (PF01497; HMM-score: 113.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 9.68
    • Cellwall Score: 0.17
    • Extracellular Score: 0.16
    • Internal Helix: 1
  • LocateP: Lipid anchored
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPII)
    • Intracellular possibility: -0.33
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: LLVAACG
  • SignalP: Signal peptide LIPO(Sec/SPII) length 21 aa
    • SP(Sec/SPI): 0.000692
    • TAT(Tat/SPI): 0.000148
    • LIPO(Sec/SPII): 0.998858
    • Cleavage Site: CS pos: 21-22. VAA-CG. Pr: 0.9994
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MRGLKTFSILGLIVALLLVAACGNTDNSSKKESSTKDTISVKDENGTVKVPKDAKRIVVLEYSFADALAALDVKPVGIADDGKKKRIIKPVREKIGDYTSVGTRKQPNLEEISKLKPDLIIADSSRHKGINKELNKIAPTLSLKSFDGDYKQNINSFKTIAKALNKEKEGEKRLAEHDKLINKYKDEIKFDRNQKVLPAVVAKAGLLAHPNYSYVGQFLNELGFKNALSDDVTKGLSKYLKGPYLQLDTEHLADLNPERMIIMTDHAKKDSAEFKKLQEDATWKKLNAVKNNRVDIVDRDVWARSRGLISSEEMAKELVELSKKEQK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Lipoprotein [1] [2] [3] [4] [5]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: Fur* (repression) regulon
    Fur*(TF)important in Iron homeostasis; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: 20.04 h [6]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J Proteome Res: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. ↑ Annette Dreisbach, Kristina Hempel, Girbe Buist, Michael Hecker, Dörte Becher, Jan Maarten van Dijl
    Profiling the surfacome of Staphylococcus aureus.
    Proteomics: 2010, 10(17);3082-96
    [PubMed:20662103] [WorldCat.org] [DOI] (I p)
  4. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  5. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  6. ↑ Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SACOL2167&oldid=63317"