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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001777
  • pan locus tag?: SAUPAN004501000
  • symbol: metK
  • pan gene symbol?: metK
  • synonym:
  • product: methionine adenosyltransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001777
  • symbol: metK
  • product: methionine adenosyltransferase
  • replicon: chromosome
  • strand: -
  • coordinates: 1829028..1830218
  • length: 1191
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGTTAAATAACAAACGATTATTTACTTCAGAGTCTGTTACAGAAGGACACCCAGATAAA
    ATCGCTGACCAAGTGTCAGATGCAATATTAGATGCTATTTTAAAAGACGACCCAAATGCA
    CGTGTAGCTTGTGAAACAACTGTTACAACAGGTATGGCATTGATCGCCGGCGAAATTTCT
    ACAACAACATATGTTGATATTCCGAAAGTTGTTAGAGAAACAATTAAAGAAATTGGATAC
    ACAAGAGCAAAATATGGTTATGATTATGAAACAATGGCAATTTTAACTGCGATTGATGAA
    CAATCACCTGACATTGCACAAGGCGTGGATAAAGCATTAGAGTATCGTGATAAAGATAGC
    AAAGAAATTGAAGCAACAGGTGCAGGTGACCAAGGTTTAATGTTTGGTTACGCAACGAAT
    GAAACAGAGACGTATATGCCTCTAGCAATTTATTTATCACATCAATTGGCAAAACGTTTA
    TCAGATGTGCGTAAAGATGGTACGTTAAATTATTTACGACCAGACGGTAAAGTTCAGGTC
    ACTGTTGAATATGATGAAAATGATAACCCTGTACGTATTGATACGATTGTCGTATCAACA
    CAACATGCTGAGGATGTGACACTTGAACAAATTCAAGAAGACATTAAAGCTCATGTCATT
    TATCCAACTGTTCCAGAAAACTTGATTAATGAACAAACTAAATTCTACATCAATCCTACA
    GGACGCTTTGTAATCGGTGGACCTCAAGGTGATGCAGGATTAACAGGACGTAAAATCATT
    GTTGATACTTACGGTGGCTATGCACGTCATGGTGGCGGATGCTTCAGTGGTAAAGATCCT
    ACAAAAGTAGACCGTTCAGCTGCATACGCTGCACGTTATGTGGCTAAAAATATTGTTGCA
    GCAGGTTTAGCAGATCAATGTGAAGTACAATTGGCATATGCGATTGGTGTTGCAGAACCA
    GTGTCAATTGCAATTGATACATTTGGAACAGGTAAAGTTTCTGAAGGGCAACTTGTTGAA
    GCAGTTAGAAAACACTTTGACCTAAGACCTGCTGGTATTATTAAAATGCTTGATTTGAAA
    CAACCGATTTACAAACAAACTGCTGCTTATGGTCATTTTGGACGTACAGATGTATTATTC
    CCATGGGAAAAATTAGACAAAGTGGAAGAATTAAAAGACGCAGTAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1191

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_001777
  • symbol: MetK
  • description: methionine adenosyltransferase
  • length: 396
  • theoretical pI: 4.74809
  • theoretical MW: 43510.8
  • GRAVY: -0.318434

⊟Function[edit | edit source]

  • reaction:
    EC 2.5.1.6?  ExPASy
    Methionine adenosyltransferase ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine
  • TIGRFAM:
    Metabolism Central intermediary metabolism Other methionine adenosyltransferase (TIGR01034; EC 2.5.1.6; HMM-score: 621.7)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    no clan defined S-AdoMet_synt_C; S-adenosylmethionine synthetase, C-terminal domain (PF02773; HMM-score: 238.6)
    and 2 more
    S-AdoMet_synt_M; S-adenosylmethionine synthetase, central domain (PF02772; HMM-score: 174.4)
    S-AdoMet_synt_N; S-adenosylmethionine synthetase, N-terminal domain (PF00438; HMM-score: 151.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9589
    • Cytoplasmic Membrane Score: 0.0052
    • Cell wall & surface Score: 0.0006
    • Extracellular Score: 0.0353
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.014999
    • TAT(Tat/SPI): 0.000791
    • LIPO(Sec/SPII): 0.001932
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MLNNKRLFTSESVTEGHPDKIADQVSDAILDAILKDDPNARVACETTVTTGMALIAGEISTTTYVDIPKVVRETIKEIGYTRAKYGYDYETMAILTAIDEQSPDIAQGVDKALEYRDKDSKEIEATGAGDQGLMFGYATNETETYMPLAIYLSHQLAKRLSDVRKDGTLNYLRPDGKVQVTVEYDENDNPVRIDTIVVSTQHAEDVTLEQIQEDIKAHVIYPTVPENLINEQTKFYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGCFSGKDPTKVDRSAAYAARYVAKNIVAAGLADQCEVQLAYAIGVAEPVSIAIDTFGTGKVSEGQLVEAVRKHFDLRPAGIIKMLDLKQPIYKQTAAYGHFGRTDVLFPWEKLDKVEELKDAVK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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