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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001756
  • pan locus tag?: SAUPAN004454000
  • symbol: JSNZ_001756
  • pan gene symbol?: putA
  • synonym:
  • product: proline dehydrogenase family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001756
  • symbol: JSNZ_001756
  • product: proline dehydrogenase family protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1809427..1810428
  • length: 1002
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGGCACTATTAAAGAATTTTTTTATCGGATTATCTAATAATAGTTTTTTAAACAACGCA
    GCAAAAAAAGTGGGCCCACGTTTGGGCGCCAATAAAGTCGTTGCCGGAAATACAATTCCA
    GAGTTAATTAATACAATCGAATACTTAAATGACAAGAATATCGCTGTTACGGTAGACAAT
    TTAGGGGAATTTGTCGGTACAGTTGAAGAAAGTAATCATGCTAAAGAACAAATTTTAACA
    ATTATGGACGCGCTTCATCAACATGGCGTAAAGGCACATATGTCTGTTAAATTGAGTCAG
    TTAGGTGCAGAATTCGACTTAGAATTAGCTTACCAAAATTTAAGAGAGATTTTACTTAAA
    GCAAATACTTACAACAATATGCATATAAATATTGATACTGAAAAATATGCTAGCCTGCAA
    CAAATTGTTCAAGTTTTAGATCGCTTAAAAGGCGAATTTAGAAATGTTGGTACTGTAATT
    CAAGCATATTTATACGATAGCCACGAATTAGTTGATAAGTACCAAGATTTACGATTACGT
    TTGGTTAAAGGTGCATATAAAGAAAACGAATCAATTGCATTTCAATCTAAGGAAGACGTA
    GATGCAAATTACATCAAAATAATTGAACAACGTTTGTTAAACGCACGCAATTTCACTTCA
    ATTGCAACACATGACCATCGCATCATTAATCATGTAAAACAATTTATGAAAGAAAATCAC
    ATTGAAAAAGATCGTATGGAATTCCAAATGCTCTATGGTTTTAGATCAGAGTTAGCAGAA
    GAAATCGCAAATGAAGGCTATAATTTCACTATTTATGTACCTTATGGCGATGATTGGTTT
    GCGTATTTTATGAGAAGATTAGCAGAACGCCCACAAAACCTATCTCTTGCTGTAAAAGAA
    TTTGTGAAACCTGCTGGCTTAAAACGTGTTGGCATAATTGCAGCTTTAGGAGTTACAGTT
    ATGTTAGGTTTAAGTACAATTAAAAAATTATGCCGTAAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1002

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_001756
  • symbol: JSNZ_001756
  • description: proline dehydrogenase family protein
  • length: 333
  • theoretical pI: 8.36497
  • theoretical MW: 38057.5
  • GRAVY: -0.236937

⊟Function[edit | edit source]

  • TIGRFAM:
    acidobacterial duplicated orphan permease (TIGR03434; HMM-score: 15.5)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    TIM_barrel (CL0036) Pro_dh; Proline dehydrogenase (PF01619; HMM-score: 126.7)
    and 4 more
    no clan defined SPATA2_PUB-like; Spermatogenesis-associated protein 2, PUB-like domain (PF21388; HMM-score: 15.4)
    PorB; Alpha helical Porin B (PF11565; HMM-score: 13.9)
    Acetyltrans (CL0257) NMT; Myristoyl-CoA:protein N-myristoyltransferase, N-terminal domain (PF01233; HMM-score: 12.8)
    TIM_barrel (CL0036) Radical_SAM; Radical SAM superfamily (PF04055; HMM-score: 12.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.0368
    • Cytoplasmic Membrane Score: 0.7035
    • Cell wall & surface Score: 0.0028
    • Extracellular Score: 0.257
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.015058
    • TAT(Tat/SPI): 0.002094
    • LIPO(Sec/SPII): 0.001817
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MALLKNFFIGLSNNSFLNNAAKKVGPRLGANKVVAGNTIPELINTIEYLNDKNIAVTVDNLGEFVGTVEESNHAKEQILTIMDALHQHGVKAHMSVKLSQLGAEFDLELAYQNLREILLKANTYNNMHINIDTEKYASLQQIVQVLDRLKGEFRNVGTVIQAYLYDSHELVDKYQDLRLRLVKGAYKENESIAFQSKEDVDANYIKIIEQRLLNARNFTSIATHDHRIINHVKQFMKENHIEKDRMEFQMLYGFRSELAEEIANEGYNFTIYVPYGDDWFAYFMRRLAERPQNLSLAVKEFVKPAGLKRVGIIAALGVTVMLGLSTIKKLCRK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA regulon
    CcpA(TF)important in Carbon catabolism;  regulation predicted or transferred from N315 and NCTC 8325  [1]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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