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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001641
  • pan locus tag?: SAUPAN004258000
  • symbol: JSNZ_001641
  • pan gene symbol?: —
  • synonym:
  • product: DUF4930 family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001641
  • symbol: JSNZ_001641
  • product: DUF4930 family protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1675854..1676327
  • length: 474
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    ATGAGATTGATATTTAGTATGATTAAAAACATTATAGCAGTAATAGCAATTGTGATAATT
    GTTTATATCGCCTTGAAATACGCACCTTTTTTAAAAGAACAAGATTGGAATCCTGTAAAT
    CACGATAATGATCAAATGAATCAAGTTACGCAACCCACTAATGATGCCCAACATGTTTAT
    GTAAGTGGTGAGAAATATTCATTAAAAGAAAATGATTTAATAAAGAATGTACCATTAAGC
    CAAATTAAAAATGTATTTAAAATGATAGATAAACAAGAGTTTATGGCTGTGTCTGGAATG
    AATCGCATGGCTTATAATGATCAATATATTATAGGTCAAAGAGGAGATGAATTTATTCTT
    TATAAATTTGGAGATGAGTCAATGCGTGTTTACAATACTGAATTTGAAATGCAACAAGAC
    TTAAATGAATTAGGGCAAAATTTACAACTAAAACCTGAAAATGCTTATCAATAG
    60
    120
    180
    240
    300
    360
    420
    474

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_001641
  • symbol: JSNZ_001641
  • description: DUF4930 family protein
  • length: 157
  • theoretical pI: 4.76604
  • theoretical MW: 18395
  • GRAVY: -0.395541

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    no clan defined DUF4930; Domain of unknown function (DUF4930) (PF16284; HMM-score: 265.1)
    and 3 more
    TrbC_VirB2 (CL0690) T4SS_pilin; Type IV secretion system pilin (PF18895; HMM-score: 14.2)
    no clan defined DUF3975; Protein of unknown function (DUF3975) (PF13126; HMM-score: 13.2)
    DUF6040; Family of unknown function (DUF6040) (PF19506; HMM-score: 13.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.32
    • Cytoplasmic Membrane Score: 9.55
    • Cellwall Score: 0.12
    • Extracellular Score: 0.01
    • Internal Helix: 1
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.0003
    • Cytoplasmic Membrane Score: 0.9237
    • Cell wall & surface Score: 0.0005
    • Extracellular Score: 0.0756
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.222384
    • TAT(Tat/SPI): 0.000427
    • LIPO(Sec/SPII): 0.012959
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MRLIFSMIKNIIAVIAIVIIVYIALKYAPFLKEQDWNPVNHDNDQMNQVTQPTNDAQHVYVSGEKYSLKENDLIKNVPLSQIKNVFKMIDKQEFMAVSGMNRMAYNDQYIIGQRGDEFILYKFGDESMRVYNTEFEMQQDLNELGQNLQLKPENAYQ

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: VraR (activation) regulon
    VraR(TF)response regulator important for resistance against cell-wall targeting antibiotics;  [1] [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Makoto Kuroda, Hiroko Kuroda, Taku Oshima, Fumihiko Takeuchi, Hirotada Mori, Keiichi Hiramatsu
    Two-component system VraSR positively modulates the regulation of cell-wall biosynthesis pathway in Staphylococcus aureus.
    Mol Microbiol: 2003, 49(3);807-21
    [PubMed:12864861] [WorldCat.org] [DOI] (P p)
  2. ↑ Susan Boyle-Vavra, Shouhui Yin, Dae Sun Jo, Christopher P Montgomery, Robert S Daum
    VraT/YvqF is required for methicillin resistance and activation of the VraSR regulon in Staphylococcus aureus.
    Antimicrob Agents Chemother: 2013, 57(1);83-95
    [PubMed:23070169] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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