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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001510
  • pan locus tag?: SAUPAN004074000
  • symbol: recN
  • pan gene symbol?: recN
  • synonym:
  • product: DNA repair protein RecN

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001510
  • symbol: recN
  • product: DNA repair protein RecN
  • replicon: chromosome
  • strand: -
  • coordinates: 1553301..1554980
  • length: 1680
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGTTACAAACCTTATCAATCAAGCAATTTGCTATTATTGAAGAGTTAGAAATTCAATTT
    TCTGATGGTTTAACTGTTTTAAGTGGTGAAACGGGTTCAGGTAAGTCAATTATTATAGAT
    GCAATAGGACAATTAATTGGAATGAGAGCTTCATCTGATTTTGTAAGACATGGTGAAAAA
    AAGGCTGTCATCGAAGGTATATTTGATATTGACGAGAGTAAAGATGCTATACATATATTG
    AAAAATATGGATATTGATGTAGATGAAGATTTTTTATTAGTTAAACGGGAAATTTTCAGC
    TCTGGTAAAAGTCTTTGTAAAATTAATAATCAAACAGTTACTTTACAAGATTTACGAAAA
    GTTATGCAAGAATTATTAGATATTCATGGCCAACATGAAACGCAGTCTTTGTTGAAACAA
    AAATACCATTTAACATTATTGGATAATTACGCAGAATCACGTTATCAAGATTTGTTGGAT
    AAATATCATCAAACTTTTCAAAATTATAAAGCCAAAAAGCAAGAGTTAGAAGATTTAGAA
    TCAGCAGACCAAGCATTGCTGCAGCGTTTAGATTTAATGAAATTCCAATTAGAAGAATTG
    TCTGAAGCACATCTAAAAGAAGGCGAAATAGAGCAACTTGAAATAGATATTAAACGCATT
    CAAAACTCTGAAAAATTAAGCTTAGCGCTTAATAACGCACATATGACATTAACGGATGAA
    AATGCTATCACTGATCGTTTGTATGAACTAAGCAACCATTTGTTAACAATAAACGATATA
    GTTCCAAACAAATATGACAAATTAAAAGAAGATATTGATCAATTTTACTACATTTTAGAA
    GATGCAAAACATGAGTTATATGATGAAATGGCTAATACAGAATTCGACGAACAAGTGTTA
    AACGAGTATGAATCTCGTATGAATTTGCTGAATAATTTAAAACGTAAATATGGAAAAGAT
    ATTTCAGAATTAATCGCATATCAAGAAAAGCTTAATAATGAAATCAATAAAATTGAAAAC
    TATGAACAAAGTACATCGCAGTTACGAGAAGAAATAAATGCATTGTATAATCAAGTTATA
    GAGGTTGGACAAGCGTTATCAAAGCAGCGTCGTATAGTCGCTAGGGAGTTAAGAGACCAC
    ATTGTATCGGAAATTCAAAACTTACAAATGAAAGACGCAAATCTTGAGATTTCATTTAAA
    AAATTAGAAGAACCGAATATTGATGGAATCGAATTTGTAGAATTTTTAATCAGTCCAAAT
    AAAGGGGAACCATTAAAAAGTTTAAATAAAATTGCGTCAGGTGGAGAACTTTCTAGAATT
    ATGTTAGCGTTAAAAAGTATTTTTGTTAAATCGAGAGGTCAAACTGCAATTTTATTCGAT
    GAGGTTGACTCAGGTGTATCTGGACAAGCTGCACAAAAAATGGCAGAGAAAATGCGAGAT
    ATAGCAGAATATATACAAGTTATTTGTATCTCTCACTTACCACAAGTTGCATCGATGAGC
    GACCATCATTTATTAATTTCGAAATCATCAAAAGATGATCGTACAACAACACAAGTTCAA
    GAGTTAATTAGCGATGATAAAGTAGATGAAATTGCACGAATGATTTCAGGTGCAAGTGTT
    ACTGATTTAACTCGAGAAAATGCAAGAGAAATGATACAACACAATCAAAGACGTAGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_001510
  • symbol: RecN
  • description: DNA repair protein RecN
  • length: 559
  • theoretical pI: 4.73168
  • theoretical MW: 64295.4
  • GRAVY: -0.502147

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA repair protein RecN (TIGR00634; HMM-score: 546)
    and 12 more
    Cellular processes Cellular processes Cell division chromosome segregation protein SMC (TIGR02169; HMM-score: 45.5)
    Genetic information processing DNA metabolism Chromosome-associated proteins chromosome segregation protein SMC (TIGR02169; HMM-score: 45.5)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA replication and repair protein RecF (TIGR00611; HMM-score: 28.7)
    thiol reductant ABC exporter, CydD subunit (TIGR02857; HMM-score: 23.1)
    Cellular processes Cellular processes Cell division chromosome segregation protein SMC (TIGR02168; HMM-score: 20.8)
    Genetic information processing DNA metabolism Chromosome-associated proteins chromosome segregation protein SMC (TIGR02168; HMM-score: 20.8)
    phosphonate C-P lyase system protein PhnL (TIGR02324; HMM-score: 13.3)
    Metabolism Transport and binding proteins Amino acids, peptides and amines urea ABC transporter, ATP-binding protein UrtD (TIGR03411; HMM-score: 8.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Molybdopterin MoaD family protein (TIGR01687; HMM-score: 6.7)
    Metabolism Transport and binding proteins Cations and iron carrying compounds potassium uptake protein (TIGR00794; HMM-score: 6.1)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking type I secretion membrane fusion protein, HlyD family (TIGR01843; HMM-score: 4.2)
    thiazole/oxazole-forming peptide maturase, SagD family component (TIGR03604; HMM-score: 3.4)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    P-loop_NTPase (CL0023) SMC_N; RecF/RecN/SMC N terminal domain (PF02463; HMM-score: 74.4)
    and 13 more
    AAA_23; AAA domain (PF13476; HMM-score: 52.5)
    AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 24.3)
    AAA_21; AAA domain, putative AbiEii toxin, Type IV TA system (PF13304; HMM-score: 22.4)
    AAA_15; AAA ATPase domain (PF13175; HMM-score: 17)
    no clan defined DUF6549; Family of unknown function (DUF6549) (PF20186; HMM-score: 16.8)
    P-loop_NTPase (CL0023) AAA_22; AAA domain (PF13401; HMM-score: 16.1)
    AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 14.4)
    AAA_16; AAA ATPase domain (PF13191; HMM-score: 13.5)
    RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 13.4)
    GH57_38_middle (CL0599) BE_C; 1,4-alpha-glucan branching enzyme, C-terminal (PF09210; HMM-score: 13.4)
    P-loop_NTPase (CL0023) DUF5906; Family of unknown function (DUF5906) (PF19263; HMM-score: 12.2)
    no clan defined OVT1; Major antigen, helical domain (PF24423; HMM-score: 11.4)
    DUF6822; Family of unknown function (DUF6822) (PF20708; HMM-score: 5.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9901
    • Cytoplasmic Membrane Score: 0.009
    • Cell wall & surface Score: 0.0003
    • Extracellular Score: 0.0006
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002732
    • TAT(Tat/SPI): 0.000297
    • LIPO(Sec/SPII): 0.000424
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MLQTLSIKQFAIIEELEIQFSDGLTVLSGETGSGKSIIIDAIGQLIGMRASSDFVRHGEKKAVIEGIFDIDESKDAIHILKNMDIDVDEDFLLVKREIFSSGKSLCKINNQTVTLQDLRKVMQELLDIHGQHETQSLLKQKYHLTLLDNYAESRYQDLLDKYHQTFQNYKAKKQELEDLESADQALLQRLDLMKFQLEELSEAHLKEGEIEQLEIDIKRIQNSEKLSLALNNAHMTLTDENAITDRLYELSNHLLTINDIVPNKYDKLKEDIDQFYYILEDAKHELYDEMANTEFDEQVLNEYESRMNLLNNLKRKYGKDISELIAYQEKLNNEINKIENYEQSTSQLREEINALYNQVIEVGQALSKQRRIVARELRDHIVSEIQNLQMKDANLEISFKKLEEPNIDGIEFVEFLISPNKGEPLKSLNKIASGGELSRIMLALKSIFVKSRGQTAILFDEVDSGVSGQAAQKMAEKMRDIAEYIQVICISHLPQVASMSDHHLLISKSSKDDRTTTQVQELISDDKVDEIARMISGASVTDLTRENAREMIQHNQRRR

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: ArgR/AhrC (repression) regulon
    ArgR/AhrC(TF)important in Arginine biosynthesis, Arginine degradation;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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