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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001257
  • pan locus tag?: SAUPAN003587000
  • symbol: recA
  • pan gene symbol?: recA
  • synonym:
  • product: recombinase RecA

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001257
  • symbol: recA
  • product: recombinase RecA
  • replicon: chromosome
  • strand: +
  • coordinates: 1270818..1271861
  • length: 1044
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    TTGGATAACGATCGTCAAAAAGCTTTAGATACAGTAATTAAAAATATGGAGAAATCTTTC
    GGTAAAGGTGCCGTAATGAAGTTGGGTGACAATATAGGTCGCCGAGTTTCAACTACATCA
    ACTGGTTCAGTTACATTAGATAATGCGCTAGGTGTAGGTGGCTATCCTAAAGGACGAATT
    ATTGAAATTTATGGTCCTGAAAGTTCTGGTAAGACAACAGTAGCGCTTCACGCTATTGCT
    GAAGTACAAAGTAATGGCGGGGTGGCAGCATTTATCGATGCTGAACATGCTTTAGATCCA
    GAATATGCTCAAGCATTAGGCGTAGATATCGATAATTTATATTTATCGCAACCGGATCAT
    GGTGAACAAGGTCTTGAAATCGCCGAAGCATTTGTTAGAAGTGGTGCAGTTGATATTGTA
    GTTGTAGACTCAGTTGCTGCTTTAACACCTAAAGCTGAAATTGAAGGAGAAATGGGAGAC
    ACTCACGTTGGTTTACAAGCTCGTTTAATGTCACAAGCGTTACGTAAACTTTCAGGTGCT
    ATTTCTAAATCAAATACAACTGCTATTTTCATCAACCAAATTCGTGAAAAAGTTGGTGTT
    ATGTTCGGTAATCCAGAGACTACACCAGGTGGACGTGCATTAAAATTCTATAGTTCAGTA
    AGACTAGAAGTACGTCGTGCAGAACAGCTTAAACAAGGACAAGAAATTGTAGGTAATAGA
    ACTAAAATTAAAGTCGTTAAAAATAAAGTGGCACCACCATTTAGAGTAGCTGAAGTTGAT
    ATTATGTATGGACAAGGTATTTCTAAAGAGGGTGAACTTATTGATTTAGGTGTTGAAAAC
    GACATCGTTGATAAATCAGGAGCATGGTATTCTTACAATGGTGAACGAATGGGTCAAGGT
    AAGGAAAATGTTAAAATGTACTTGAAAGAAAATCCACAAATTAAAGAAGAAATTGATCGT
    AAATTGAGAGAAAAATTAGGGATATCTGATGGTGATGTTGAAGAAACAGAAGATGCACCA
    AAGTCATTATTTGACGAAGAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1044

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_001257
  • symbol: RecA
  • description: recombinase RecA
  • length: 347
  • theoretical pI: 4.85109
  • theoretical MW: 37656.3
  • GRAVY: -0.398847

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair protein RecA (TIGR02012; HMM-score: 565.3)
    and 11 more
    DNA repair and recombination protein RadB (TIGR02237; HMM-score: 60.3)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA repair and recombination protein RadA (TIGR02236; HMM-score: 41.2)
    meiotic recombinase Dmc1 (TIGR02238; HMM-score: 36.9)
    DNA repair protein RAD51 (TIGR02239; HMM-score: 31.4)
    KaiC domain protein, PAE1156 family (TIGR03881; HMM-score: 20.6)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA repair protein RadA (TIGR00416; HMM-score: 16.5)
    KaiC domain protein, AF_0351 family (TIGR03880; HMM-score: 16.2)
    2-aminoethylphosphonate ABC transport system, ATP-binding component PhnT (TIGR03258; HMM-score: 14)
    Metabolism Transport and binding proteins Unknown substrate energy-coupling factor transporter ATPase (TIGR04520; EC 3.6.3.-; HMM-score: 12.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pantothenate and coenzyme A phosphopantothenoylcysteine decarboxylase / phosphopantothenate--cysteine ligase (TIGR00521; EC 4.1.1.36,6.3.2.5; HMM-score: 11.4)
    putative cytidylate kinase (TIGR02173; EC 2.7.4.14; HMM-score: 10.5)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    P-loop_NTPase (CL0023) RecA; recA bacterial DNA recombination protein (PF00154; HMM-score: 472.4)
    and 10 more
    no clan defined RecA_C; RecA C-terminal domain (PF21096; HMM-score: 97.9)
    P-loop_NTPase (CL0023) Rad51; Rad51 (PF08423; HMM-score: 38.1)
    ATPase; KaiC (PF06745; HMM-score: 35.3)
    ATPase_2; ATPase domain predominantly from Archaea (PF01637; HMM-score: 21.1)
    AAA_16; AAA ATPase domain (PF13191; HMM-score: 18.7)
    AAA_25; AAA domain (PF13481; HMM-score: 18)
    AAA_24; AAA domain (PF13479; HMM-score: 15.9)
    H-int (CL0363) PT-HINT; Pretoxin HINT domain (PF07591; HMM-score: 13.3)
    NADP_Rossmann (CL0063) DFP; DNA / pantothenate metabolism flavoprotein C-terminal (PF04127; HMM-score: 12.4)
    P-loop_NTPase (CL0023) AAA_22; AAA domain (PF13401; HMM-score: 12.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9595
    • Cytoplasmic Membrane Score: 0.0099
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0306
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.048833
    • TAT(Tat/SPI): 0.065314
    • LIPO(Sec/SPII): 0.00261
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MDNDRQKALDTVIKNMEKSFGKGAVMKLGDNIGRRVSTTSTGSVTLDNALGVGGYPKGRIIEIYGPESSGKTTVALHAIAEVQSNGGVAAFIDAEHALDPEYAQALGVDIDNLYLSQPDHGEQGLEIAEAFVRSGAVDIVVVDSVAALTPKAEIEGEMGDTHVGLQARLMSQALRKLSGAISKSNTTAIFINQIREKVGVMFGNPETTPGGRALKFYSSVRLEVRRAEQLKQGQEIVGNRTKIKVVKNKVAPPFRVAEVDIMYGQGISKEGELIDLGVENDIVDKSGAWYSYNGERMGQGKENVKMYLKENPQIKEEIDRKLREKLGISDGDVEETEDAPKSLFDEE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: LexA (repression) regulon
    LexA(TF)important in SOS response;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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