PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_001257
• pan locus tag^?: SAUPAN003587000
• symbol: recA
• pan gene symbol^?: recA
• synonym:
• product: recombinase RecA

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_001257
• symbol: recA
• product: recombinase RecA
• replicon: chromosome
• strand: +
• coordinates: 1270818..1271861
• length: 1044
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  
  TTGGATAACGATCGTCAAAAAGCTTTAGATACAGTAATTAAAAATATGGAGAAATCTTTC
  GGTAAAGGTGCCGTAATGAAGTTGGGTGACAATATAGGTCGCCGAGTTTCAACTACATCA
  ACTGGTTCAGTTACATTAGATAATGCGCTAGGTGTAGGTGGCTATCCTAAAGGACGAATT
  ATTGAAATTTATGGTCCTGAAAGTTCTGGTAAGACAACAGTAGCGCTTCACGCTATTGCT
  GAAGTACAAAGTAATGGCGGGGTGGCAGCATTTATCGATGCTGAACATGCTTTAGATCCA
  GAATATGCTCAAGCATTAGGCGTAGATATCGATAATTTATATTTATCGCAACCGGATCAT
  GGTGAACAAGGTCTTGAAATCGCCGAAGCATTTGTTAGAAGTGGTGCAGTTGATATTGTA
  GTTGTAGACTCAGTTGCTGCTTTAACACCTAAAGCTGAAATTGAAGGAGAAATGGGAGAC
  ACTCACGTTGGTTTACAAGCTCGTTTAATGTCACAAGCGTTACGTAAACTTTCAGGTGCT
  ATTTCTAAATCAAATACAACTGCTATTTTCATCAACCAAATTCGTGAAAAAGTTGGTGTT
  ATGTTCGGTAATCCAGAGACTACACCAGGTGGACGTGCATTAAAATTCTATAGTTCAGTA
  AGACTAGAAGTACGTCGTGCAGAACAGCTTAAACAAGGACAAGAAATTGTAGGTAATAGA
  ACTAAAATTAAAGTCGTTAAAAATAAAGTGGCACCACCATTTAGAGTAGCTGAAGTTGAT
  ATTATGTATGGACAAGGTATTTCTAAAGAGGGTGAACTTATTGATTTAGGTGTTGAAAAC
  GACATCGTTGATAAATCAGGAGCATGGTATTCTTACAATGGTGAACGAATGGGTCAAGGT
  AAGGAAAATGTTAAAATGTACTTGAAAGAAAATCCACAAATTAAAGAAGAAATTGATCGT
  AAATTGAGAGAAAAATTAGGGATATCTGATGGTGATGTTGAAGAAACAGAAGATGCACCA
  AAGTCATTATTTGACGAAGAATAG
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1044
  

⊟Protein

⊟General

• locus tag: JSNZ_001257
• symbol: RecA
• description: recombinase RecA
• length: 347
• theoretical pI: 4.85109
• theoretical MW: 37656.3
• GRAVY: -0.398847

⊟Function

• TIGRFAM:
  Genetic information processing DNA metabolism DNA replication, recombination, and repair protein RecA (TIGR02012; HMM-score: 565.3)
  and 11 more
  DNA repair and recombination protein RadB (TIGR02237; HMM-score: 60.3)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA repair and recombination protein RadA (TIGR02236; HMM-score: 41.2)
  meiotic recombinase Dmc1 (TIGR02238; HMM-score: 36.9)
  DNA repair protein RAD51 (TIGR02239; HMM-score: 31.4)
  KaiC domain protein, PAE1156 family (TIGR03881; HMM-score: 20.6)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA repair protein RadA (TIGR00416; HMM-score: 16.5)
  KaiC domain protein, AF_0351 family (TIGR03880; HMM-score: 16.2)
  2-aminoethylphosphonate ABC transport system, ATP-binding component PhnT (TIGR03258; HMM-score: 14)
  Metabolism Transport and binding proteins Unknown substrate energy-coupling factor transporter ATPase (TIGR04520; EC 3.6.3.-; HMM-score: 12.8)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pantothenate and coenzyme A phosphopantothenoylcysteine decarboxylase / phosphopantothenate--cysteine ligase (TIGR00521; EC 4.1.1.36,6.3.2.5; HMM-score: 11.4)
  putative cytidylate kinase (TIGR02173; EC 2.7.4.14; HMM-score: 10.5)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  P-loop_NTPase (CL0023) RecA; recA bacterial DNA recombination protein (PF00154; HMM-score: 472.4)
  and 10 more
  no clan defined RecA_C; RecA C-terminal domain (PF21096; HMM-score: 97.9)
  P-loop_NTPase (CL0023) Rad51; Rad51 (PF08423; HMM-score: 38.1)
  ATPase; KaiC (PF06745; HMM-score: 35.3)
  ATPase_2; ATPase domain predominantly from Archaea (PF01637; HMM-score: 21.1)
  AAA_16; AAA ATPase domain (PF13191; HMM-score: 18.7)
  AAA_25; AAA domain (PF13481; HMM-score: 18)
  AAA_24; AAA domain (PF13479; HMM-score: 15.9)
  H-int (CL0363) PT-HINT; Pretoxin HINT domain (PF07591; HMM-score: 13.3)
  NADP_Rossmann (CL0063) DFP; DNA / pantothenate metabolism flavoprotein C-terminal (PF04127; HMM-score: 12.4)
  P-loop_NTPase (CL0023) AAA_22; AAA domain (PF13401; HMM-score: 12.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9595
  • Cytoplasmic Membrane Score: 0.0099
  • Cell wall & surface Score: 0
  • Extracellular Score: 0.0306
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.048833
  • TAT(Tat/SPI): 0.065314
  • LIPO(Sec/SPII): 0.00261
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MDNDRQKALDTVIKNMEKSFGKGAVMKLGDNIGRRVSTTSTGSVTLDNALGVGGYPKGRIIEIYGPESSGKTTVALHAIAEVQSNGGVAAFIDAEHALDPEYAQALGVDIDNLYLSQPDHGEQGLEIAEAFVRSGAVDIVVVDSVAALTPKAEIEGEMGDTHVGLQARLMSQALRKLSGAISKSNTTAIFINQIREKVGVMFGNPETTPGGRALKFYSSVRLEVRRAEQLKQGQEIVGNRTKIKVVKNKVAPPFRVAEVDIMYGQGISKEGELIDLGVENDIVDKSGAWYSYNGERMGQGKENVKMYLKENPQIKEEIDRKLREKLGISDGDVEETEDAPKSLFDEE

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for N315

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications