PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_001186
• pan locus tag^?: SAUPAN003500000
• symbol: rsmB
• pan gene symbol^?: sun
• synonym:
• product: 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_001186
• symbol: rsmB
• product: 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB
• replicon: chromosome
• strand: +
• coordinates: 1189084..1190391
• length: 1308
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  
  ATGATAGAAAACGTGAGAAGTCTTGCTTTTGACACGATTCAAGATATATTAAATGAAGGT
  GCGTATAGTAACTTGCGTATTAATGAAGTGTTGTCAGAAAATGAATTAAATGCAATGGAT
  AAAGCCTTATTTACAGAAATTGTCTACGGAACCGTTAAAAGAAAATATACGTTAGATTTT
  TATTTAAAGCCTTTTGTGAAAACAAAGATTAAGGCATGGGTTAGGCAATTATTATGGATG
  AGTATTTATCAATATGTATATTTAGATAAAGTTCCAAATCATGCCATTATTAATGAAGCA
  GTTGAAATAGCAAAAGAACGCGGTGGCTATCATAATGGTAATGTCGTAAATGGTATTTTA
  CGTACAATGATGCGTAGTGACTTACCTGATTTTAATGAAATTGCAGATCCTAAAAAAAGA
  ATGGCAATCGAGTATAGTATGCCGAAGTGGATTATAGATCATTGGGCAACACATTATGGT
  CTCGAAGAAACTGAAACAATTTTACAGTCATTTTTAGAAACGACATCAACAACTGTGCGT
  GCCAACCTGACGCGAGCATCATTAGATGATATTATTGAAAAGTTGCAAGACGAAGGTTAT
  GACGTTGAAAAAGATCATGACTTACCTTATTGTCTCCATATAGGAGGACAACCAATTATT
  CATTCTCGTTCATTTAAAGATGGATTCGTTTCAATTCAAGATAAAAGCTCAATGTTTGTT
  GCACACATTATGAATGTAGACCGACATGATCACGTATTAGATGCATGTAGTGCACCTGGC
  GGTAAAGCTTGTCACATTGCTGAAGTTTTAATGCCAGAAGGGCAAGTTGACGCTTCAGAT
  ATACATGATCACAAAATAGACTTAATTAATTTTAATATAAAAAAATTACGATTAACAAAT
  ATTAAAGCTTTTCAACATGATGCGACAAAACCTTATGATAAAACATACGATAAGATACTT
  GTTGATGCACCATGTAGCGGATTAGGTGTAATGAGACATAAGCCGGAGATTAAGTATACT
  CAAAGCAAACAACATATTGAGTCACTAGTTGAATTACAGCTTGAAATATTGGAAAATGTA
  AAAAACAATGTAAAAATAGGTGGCGAAATCATCTATTCAACATGTACAATTGAGCAACTA
  GAAAATGAAAACGTGATTTATACGTTTTTGAAAAATAATAAAAACTTCGAATTTGAACCG
  TTTCAACATCCGATAACTGGAGAGTTGGTCAAAACGTTACAAATCATGCCGCAAGACTTT
  AATTCAGATGGATTCTTTATCACTAAGATAAAAAGAAAGGACAATTAG
  60
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  1020
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  1140
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  1260
  1308
  

⊟Protein

⊟General

• locus tag: JSNZ_001186
• symbol: RsmB
• description: 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB
• length: 435
• theoretical pI: 6.06131
• theoretical MW: 50086.9
• GRAVY: -0.368736

⊟Function

• reaction:
  EC 2.1.1.176?  ExPASy
  16S rRNA (cytosine⁹⁶⁷-C₅)-methyltransferase S-adenosyl-L-methionine + cytosine⁹⁶⁷ in 16S rRNA = S-adenosyl-L-homocysteine + 5-methylcytosine⁹⁶⁷ in 16S rRNA
• TIGRFAM:
  Genetic information processing Protein synthesis tRNA and rRNA base modification 16S rRNA (cytosine(967)-C(5))-methyltransferase (TIGR00563; EC 2.1.1.176; HMM-score: 394.4)
  and 12 more
  Genetic information processing Protein synthesis tRNA and rRNA base modification NOL1/NOP2/sun family putative RNA methylase (TIGR00446; EC 2.1.1.-; HMM-score: 151.1)
  Genetic information processing Transcription Transcription factors transcription antitermination factor NusB (TIGR01951; HMM-score: 57.4)
  Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification protein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific (TIGR03533; EC 2.1.1.-; HMM-score: 26)
  Genetic information processing Protein fate Protein modification and repair methyltransferase, HemK family (TIGR00536; HMM-score: 19.4)
  Genetic information processing Protein fate Protein modification and repair protein-(glutamine-N5) methyltransferase, release factor-specific (TIGR03534; EC 2.1.1.-; HMM-score: 19.1)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-6Y C5,15-methyltransferase (decarboxylating), CbiT subunit (TIGR02469; EC 2.1.1.132; HMM-score: 18.2)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone demethylmenaquinone methyltransferase (TIGR02752; EC 2.1.1.163; HMM-score: 17.9)
  Genetic information processing Protein synthesis tRNA and rRNA base modification N2,N2-dimethylguanosine tRNA methyltransferase (TIGR00308; EC 2.1.1.-; HMM-score: 17.6)
  Genetic information processing Protein fate Protein modification and repair protein-L-isoaspartate O-methyltransferase (TIGR00080; EC 2.1.1.77; HMM-score: 14.1)
  Signal transduction Regulatory functions DNA interactions biotin operon repressor (TIGR00122; HMM-score: 13.4)
  Genetic information processing Protein synthesis tRNA and rRNA base modification ribosomal RNA large subunit methyltransferase J (TIGR00438; EC 2.1.1.166; HMM-score: 13.1)
  methyltransferase, FxLD system (TIGR04364; HMM-score: 11.2)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  NADP_Rossmann (CL0063) Methyltr_RsmB-F; 16S rRNA methyltransferase RsmB/F (PF01189; HMM-score: 174.6)
  and 14 more
  NusB (CL0633) NusB; NusB family (PF01029; HMM-score: 116.5)
  NSUN_ferredox (CL0795) RsmF-B_ferredox; Methyltr_RsmF/B-like, ferredoxin-like domain (PF22458; HMM-score: 45.7)
  NADP_Rossmann (CL0063) Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 34.9)
  MTS; Methyltransferase small domain (PF05175; HMM-score: 17.6)
  FtsJ; FtsJ-like methyltransferase (PF01728; HMM-score: 16.8)
  NSUN_ferredox (CL0795) NSUN5_fdxn-like; NOL1/NOP2/Sun domain family member 5, ferredoxin-like domain (PF21148; HMM-score: 16.5)
  NADP_Rossmann (CL0063) Methyltransf_4; Putative methyltransferase (PF02390; HMM-score: 15.6)
  Ubiquitin (CL0072) NLE; NLE (NUC135) domain (PF08154; HMM-score: 14.7)
  NADP_Rossmann (CL0063) Ubie_methyltran; ubiE/COQ5 methyltransferase family (PF01209; HMM-score: 13.7)
  DUF938; Protein of unknown function (DUF938) (PF06080; HMM-score: 13.5)
  BMT5-like; rRNA (uridine-N3-)-methyltransferase BTM5-like (PF10354; HMM-score: 13.4)
  TRM; N2,N2-dimethylguanosine tRNA methyltransferase (PF02005; HMM-score: 12.8)
  Ubiquitin (CL0072) ASPP2-like_RA; Apoptosis-stimulating of p53 protein 2-like, N-terminal RA domain (PF21801; HMM-score: 12.3)
  no clan defined SEN1_N; SEN1 N terminal (PF12726; HMM-score: 11)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9823
  • Cytoplasmic Membrane Score: 0.0131
  • Cell wall & surface Score: 0.002
  • Extracellular Score: 0.0026
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002253
  • TAT(Tat/SPI): 0.000171
  • LIPO(Sec/SPII): 0.000532
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MIENVRSLAFDTIQDILNEGAYSNLRINEVLSENELNAMDKALFTEIVYGTVKRKYTLDFYLKPFVKTKIKAWVRQLLWMSIYQYVYLDKVPNHAIINEAVEIAKERGGYHNGNVVNGILRTMMRSDLPDFNEIADPKKRMAIEYSMPKWIIDHWATHYGLEETETILQSFLETTSTTVRANLTRASLDDIIEKLQDEGYDVEKDHDLPYCLHIGGQPIIHSRSFKDGFVSIQDKSSMFVAHIMNVDRHDHVLDACSAPGGKACHIAEVLMPEGQVDASDIHDHKIDLINFNIKKLRLTNIKAFQHDATKPYDKTYDKILVDAPCSGLGVMRHKPEIKYTQSKQHIESLVELQLEILENVKNNVKIGGEIIYSTCTIEQLENENVIYTFLKNNKNFEFEPFQHPITGELVKTLQIMPQDFNSDGFFITKIKRKDN

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications