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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001045
  • pan locus tag?: SAUPAN003301000
  • symbol: JSNZ_001045
  • pan gene symbol?: rlmQ
  • synonym:
  • product: class I SAM-dependent rRNA methyltransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001045
  • symbol: JSNZ_001045
  • product: class I SAM-dependent rRNA methyltransferase
  • replicon: chromosome
  • strand: +
  • coordinates: 1050290..1051462
  • length: 1173
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAAAATAGCAACTCTGAACAAAGGCAAAGAAACAAAATATTTTAATGGATATCCTTTA
    ATTGAAGAAGAGGATATCTATTCACAAGATCATTTAAAAGAAGGAGATATTTTTCAAATT
    GTGACTGATAAATCACAATATGTTGCAACGGCTTATGTTGGTCGCCAACATAAAGGATTA
    GGTTGGGTTCTAACATACGATAAAGCTCAAGAAATCAACACAGCTTTCTTTGTGAAATTG
    TTTAATACTGCATTAGCAGAACGTGAATATTATTTTAATATAGATGGAACAAATGCTTTT
    AGATTATTTAATGCTGAAGGTGATGGTGTTGGGGGATTAACAATCGACAATTATGATGGT
    CATTTGTTGATTCAATGGTACTCAAAAGGTATTTATAAATTTAAATATGCCATTCTTGAA
    GCGGTTAGAAAAGTATTTGATTATAAATCTATTTACGAAAAAGTAAGATTTAAAGACAGC
    GAATATAGTGGTGGTTTTGTTGAAGGAGATGCACCTGAGTTTCCAATTGTTATCGAAGAA
    AACTTCACATTTTATAATGTAGACCTTGAAGATGGTTTGATGACAGGTATCTTTTTAGAT
    CAAAAAGAAGTGCGCAAGAAATTAAGGGATCAATATGCCAAAGAACGCCATGTTTTAAAC
    TTATTTAGTTATACAGGTGCTTTTTCTGTAATAGCAGCAAGTGAGGCATCTTCAACAACA
    AGTGTAGATTTGGCTAATCGTTCTCGTAGTTTAACTGAAGAAAATTTTGGATTAAATGCT
    ATTGATCCTAAATCCCAATATATTTATGTCATGGACACTTTTGATTTCTATAAATATGCT
    GCACGACATGGACATAGTTATGACACGATCGTGATTGATCCACCTAGCTTTGCGCGTAAC
    AAAAAACGTACATTTTCAGTGCAAAAAGATTATGACAAATTAATTAATGGCGCCTTAAAT
    ATCTTATCATCTGAAGGAACATTATTGTTATGTACAAACGCAAGTGTATATCCATTAAAG
    CAATTTAAAAATACTATTAAAAAGACGCTTGAAGAGAGTGGCGTTGATTATGAATTAACT
    GAAGTTATGGGATTACCAAAAGATTTTAAAACGCATCCACATTATAAGCCATCTAAATAT
    TTAAAAGCTGTTTTTGTAAATATTAGACATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1173

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_001045
  • symbol: JSNZ_001045
  • description: class I SAM-dependent rRNA methyltransferase
  • length: 390
  • theoretical pI: 6.61614
  • theoretical MW: 44810.3
  • GRAVY: -0.418974

⊟Function[edit | edit source]

  • reaction:
    EC 2.1.1.-?  ExPASy
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA and rRNA base modification 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD (TIGR00095; EC 2.1.1.171; HMM-score: 21.5)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    NADP_Rossmann (CL0063) Methyltrans_SAM; S-adenosylmethionine-dependent methyltransferase (PF10672; HMM-score: 83.2)
    and 6 more
    PUA (CL0178) PUA_3; PUA-like domain (PF17785; HMM-score: 58.1)
    NADP_Rossmann (CL0063) Cons_hypoth95; Conserved hypothetical protein 95 (PF03602; HMM-score: 27.8)
    MTS; Methyltransferase small domain (PF05175; HMM-score: 23.6)
    TRM5-TYW2_MTfase; TRM5/TYW2 methyltransferase domain (PF02475; HMM-score: 17.3)
    N6-adenineMlase; Probable N6-adenine methyltransferase (PF10237; HMM-score: 12.2)
    RsmJ; Ribosomal RNA large subunit methyltransferase D, RlmJ (PF04378; HMM-score: 11.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.967
    • Cytoplasmic Membrane Score: 0.0172
    • Cell wall & surface Score: 0.0026
    • Extracellular Score: 0.0132
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004144
    • TAT(Tat/SPI): 0.000136
    • LIPO(Sec/SPII): 0.000446
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKIATLNKGKETKYFNGYPLIEEEDIYSQDHLKEGDIFQIVTDKSQYVATAYVGRQHKGLGWVLTYDKAQEINTAFFVKLFNTALAEREYYFNIDGTNAFRLFNAEGDGVGGLTIDNYDGHLLIQWYSKGIYKFKYAILEAVRKVFDYKSIYEKVRFKDSEYSGGFVEGDAPEFPIVIEENFTFYNVDLEDGLMTGIFLDQKEVRKKLRDQYAKERHVLNLFSYTGAFSVIAASEASSTTSVDLANRSRSLTEENFGLNAIDPKSQYIYVMDTFDFYKYAARHGHSYDTIVIDPPSFARNKKRTFSVQKDYDKLINGALNILSSEGTLLLCTNASVYPLKQFKNTIKKTLEESGVDYELTEVMGLPKDFKTHPHYKPSKYLKAVFVNIRH

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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