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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_000912
  • pan locus tag?: SAUPAN003050000
  • symbol: mnhD1
  • pan gene symbol?: mnhD1
  • synonym:
  • product: Na+/H+ antiporter Mnh1 subunit D

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_000912
  • symbol: mnhD1
  • product: Na+/H+ antiporter Mnh1 subunit D
  • replicon: chromosome
  • strand: -
  • coordinates: 907347..908843
  • length: 1497
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGATTGAAAGTAATATGCTTGTGTTAACGCTAGTTATTCCAGTAATTACAGCCATATTA
    CTTGTCTTTATTGGAAAGCGTCCTATTATAAAGCGTTATGTCGCACTAGGCGGTACATTA
    TTAACCTTGGTCGCGGCAATTATCAATTTAGCAAATGTTGTTAAACATGGACCAATACGC
    GTAGAGCTAGGTTCATGGAAAGCACCATACAGTATTGTCTTTGTATTAGACATATTCAGT
    GCCCTACTAATAATTACTAGTATTATTATTACAGCTATTGTGATTTTATACTCATACCAA
    ACAATTGGTATTGAACGTGAAAGATATTATTATTATTTTTCAGTACTATTTATGTTAATA
    GGTATCATTGGTGCATTCACAACTGGTGACATTTTCAACCTATTCGTATTTTTCGAAGTA
    TTTTTAATGTCATCTTATTTCTTATTAGTAATTGGTTCTACAAAAATTCAACTACAAGAA
    ACAATAAAATATGTTCTTGTTAACGTTGTATCGTCATCCTTCTTCGTAATGGGTGTAGCG
    ATTTTATATTCAGTAGTAGGAACATTGAATCTAGCAGATATAAGCAATAAATTAGCTAAC
    CTTTCAGCGCATGACAGTGGCTTAGTCAATATCGTTTTCATACTATTTATCTTTGTTTTT
    GCTACAAAAGCTGGCGTATTCCCAATGTTTGTTTGGTTACCTAGTGCATACTATGCACCA
    CCAATTCCAATCATTGCATTCTTTGGTGCTTTGTTAACAAAAGTTGGTGTTTATGCAATC
    GCTAGAACATTGAGCCTATTCTTTAGTGATAATGTAAGCTTTTCACATTATGTCATTTTA
    TTCTTAGCATTATTAACGATCATCTTTGGTTGTGTCGGTGCTGTTGCATATGCCAACATT
    AAAAAAATCATCCTATACAATGTCATGATTGCTGTAGGTGTGATCTTAGTCGGCGTTGCA
    ATGATGACAGAGTCAGGTATGATTGGGGCTATTTACTATACATTGCACGACATGTTAGTA
    AAACTTGCATTATTCTTGCTTATCGGAATTATGATTAAAATTACAGGCACTGCTGATTTA
    AGACAATTTGGTGGCTTAATTAAACGATATCCTGTACTTGGTTGGTCATTCTTTATCGCA
    GCATTAAGTTTAGCTGGCATTCCCCCACTCAGTGGCTTTTATGGAAAATTTTTCATTGTC
    CAATCAACATTTGAAAGAGGATTTTATTTAAGTGGTGTCATCGTCTTATTATCAAGTTTA
    GTCGTATTATATTCTGTCATTCGCATATTCCTACAAGGCTTTTTCGGTCAACCAAAAGGA
    TATGATCTTAATAACAAAGTCGACGTCAAATATTTAACTACGATTGCAATCGTTGCAGTT
    GTGATAACTGTTTTATATGGATTGTCAGCAGATTACTTATATCCAATGGTAAAAGCTGGT
    GCAGAAACATTCTACAATCCATCAACCTACGTTAAAGCTGTATTAGGAGGTAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1497

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_000912
  • symbol: MnhD1
  • description: Na+/H+ antiporter Mnh1 subunit D
  • length: 498
  • theoretical pI: 9.70179
  • theoretical MW: 54755.5
  • GRAVY: 1.12691

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds multicomponent K+:H+antiporter (TIGR00944; HMM-score: 765.1)
    and 5 more
    Metabolism Energy metabolism Electron transport proton-translocating NADH-quinone oxidoreductase, chain M (TIGR01972; HMM-score: 191.1)
    Metabolism Energy metabolism Electron transport proton-translocating NADH-quinone oxidoreductase, chain N (TIGR01770; HMM-score: 159.2)
    Metabolism Energy metabolism Electron transport proton-translocating NADH-quinone oxidoreductase, chain L (TIGR01974; EC 1.6.-.-; HMM-score: 111.6)
    Metabolism Transport and binding proteins Cations and iron carrying compounds monovalent cation:proton antiporter (TIGR00940; HMM-score: 92.3)
    NAD(P)H dehydrogenase, subunit NdhF3 family (TIGR01960; HMM-score: 74.3)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    ComplexI-N (CL0425) Proton_antipo_M; NADH:quinone oxidoreductase/Mrp antiporter, TM (PF00361; HMM-score: 174.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 14
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.9995
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0005
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.010689
    • TAT(Tat/SPI): 0.000932
    • LIPO(Sec/SPII): 0.003493
  • predicted transmembrane helices (TMHMM): 14

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MIESNMLVLTLVIPVITAILLVFIGKRPIIKRYVALGGTLLTLVAAIINLANVVKHGPIRVELGSWKAPYSIVFVLDIFSALLIITSIIITAIVILYSYQTIGIERERYYYYFSVLFMLIGIIGAFTTGDIFNLFVFFEVFLMSSYFLLVIGSTKIQLQETIKYVLVNVVSSSFFVMGVAILYSVVGTLNLADISNKLANLSAHDSGLVNIVFILFIFVFATKAGVFPMFVWLPSAYYAPPIPIIAFFGALLTKVGVYAIARTLSLFFSDNVSFSHYVILFLALLTIIFGCVGAVAYANIKKIILYNVMIAVGVILVGVAMMTESGMIGAIYYTLHDMLVKLALFLLIGIMIKITGTADLRQFGGLIKRYPVLGWSFFIAALSLAGIPPLSGFYGKFFIVQSTFERGFYLSGVIVLLSSLVVLYSVIRIFLQGFFGQPKGYDLNNKVDVKYLTTIAIVAVVITVLYGLSADYLYPMVKAGAETFYNPSTYVKAVLGGK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: ManR (activation) regulon
    ManR(TF)important in Mannose utilization;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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