PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_000771
• pan locus tag^?: SAUPAN002779000
• symbol: clfA
• pan gene symbol^?: clfA
• synonym:
• product: MSCRAMM family adhesin clumping factor ClfA

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_000771
• symbol: clfA
• product: MSCRAMM family adhesin clumping factor ClfA
• replicon: chromosome
• strand: +
• coordinates: 806417..809350
• length: 2934
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  1501
  1561
  1621
  1681
  1741
  1801
  1861
  1921
  1981
  2041
  2101
  2161
  2221
  2281
  2341
  2401
  2461
  2521
  2581
  2641
  2701
  2761
  2821
  2881
  
  ATGAATATGAAGAAAAAAGAAAAACACGCAATTCGGAAAAAATCGATTGGCGTGGCTTCA
  GTGCTTGTAGGTACGTTAATCGGTTTTGGACTACTCAGCAGTAAAGAAGCAGATGCAAGT
  GAAAATAGTGTTACGCAATCTGATAGCGCAAGTAACGAAAGCAAAAGTAATGATTCAAGT
  AGCATTAATGCTGCACCTAAAACAGACAACACAAACGTGAGTGATACTAAAACAACGTCA
  AACACTAATAATGGAGAAACGAGTGTGGCGCAAAATCCAGCACAACAGGAAACGACACAA
  TCAGCATCAACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGAAACTACTACTACG
  GCAACGAATCAAGCTAATACACCGGCAACAACTCAATCAAGCAATACAAATGCGGAGGAA
  TTAGTGAATCAAACAAGTAATGAAACGACTTCTAATGATACTAATACAGTATCATCTGTA
  AATTCACCTCAAAATTCTACAAATGCGGAAAATGTTTCAACAACGCAAGATACTTCAACT
  GAAGCAACACCTTCAAACAATGAATCAGCTCCACAGAGTACAGATGCAAGTAATAAAGAT
  GTAGTTAATCAAGCGGTTAATACAAGTGCGCCTAGAATGAGAGCATTTAGTTTAGCGGCA
  GTAGCTGCAGATGCACCGGCAGCTGGCACAGATATTACGAATCAGTTGACAGATGTGAAA
  GTTACTATTGACTCTGGTACGACTGTGTATCCGCACCAAGCAGGTTATGTCAAACTGAAT
  TATGGTTTTTCAGTGCCTAATTCTGCTGTTAAAGGTGACACATTCAAAATAACTGTACCT
  AAAGAATTAAACTTAAATGGTGTAACTTCAACTGCTAAAGTGCCACCAATTATGGCTGGA
  GATCAAGTATTGGCAAATGGTGTAATCGATAGTGATGGTAATGTTATTTATACATTTACA
  GACTATGTTGATAATAAAGAAAATGTAACAGCTAATATTACTATGCCAGCTTATATTGAC
  CCTGAAAATGTTACAAAGACAGGTAATGTGACATTGACAACTGGCATAGGAACCAATACT
  GCTAGTAAGACAGTATTAATCGACTATGAGAAATATGGACAATTCCATAATTTATCAATT
  AAAGGTACGATTGATCAAATCGATAAAACAAATAATACGTATCGCCAAACAATTTATGTC
  AATCCAAGCGGAGATAACGTTGTGTTACCTGCCTTAACAGGTAATTTAATTCCTAATACA
  AAGAGTAATGCGTTAATAGATGCAAAAAACACTGATATTAAAGTTTATAGAGTCGATAAT
  GCTAATGATTTATCTGAAAGTTATTATGTGAATCCTAGCGATTTTGAAGATGTAACTAAT
  CAAGTTAGAATTTCATTTCCAAATGCTAATCAATACAAAGTAGAATTTCCTACGGACGAT
  GACCAAATTACAACACCGTATATTGTAGTTGTTAATGGCCATATTGATCCTGCTAGTACA
  GGTGATTTAGCACTACGTTCGACATTTTATGGTTATGATTCTAATTTTATATGGAGATCT
  ATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGAT
  AAACCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGAT
  TCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCGGTTCAGAT
  TCTGGCAGTGATTCTACATCAGATAGTGGTTCAGATTCAGCGAGTGATTCAGATTCAGCA
  AGTGATTCAGACTCAGCGAGTGATTCAGATTCAGCAAGTGATTCAGATTCAGCAAGTGAT
  TCAGATTCAGCAAGTGATTCAGACTCAGCAAGTGATTCAGATTCAGCAAGTGATTCAGAT
  TCAGCAAGCGATTCAGATTCAGCGAGCGATTCAGATTCAGCGAGTGATTCCGACTCAGCG
  AGCGATTCAGACTCAGATAGTGACTCAGATTCCGATAGCGATTCCGACTCAGATAGCGAC
  TCAGATTCAGACAGCGATTCTGACTCAGACAGTGACTCAGATTCCGATAGCGATTCCGAC
  TCAGACAGTGACTCAGATTCCGATAGCGATTCCGACTCAGACAGTGACTCAGATTCCGAT
  AGCGATTCAGATTCCGACAGTGATTCCGACTCAGATAGCGATTCCGACTCAGATAGCGAC
  TCAGATTCAGACAGCGATTCAGATTCAGACAGCGATTCTGACTCAGACAGTGACTCAGAT
  TCCGATAGCGATTCAGATTCAGACAGTGATTCAGACTCAGATAGCGATTCAGATTCCGAC
  AGTGACTCAGACTCAGACAGCGATTCAGATTCCGATAGCGATTCAGATTCCGACAGTGAC
  TCAGATTCCGATAGTGACTCGGATTCAGCGAGTGATTCAGATTCCGATAGCGATTCAGAA
  TCAGATAGTGACTCAGACTCAGACAGTGATTCAGATTCAGATAGTGACTCAGACTCAGAC
  AGCGATTCAGAATCAGATAGTGACTCCGATTCAGACAGCGATTCAGAATCAGATAGTGAC
  TCCGATTCAGATAGCGATTCGGATTCAGCGAGTGATTCAGACTCAGGTAGTGACTCCGAT
  TCATCAAGTGATTCAGATTCCGATTCAACGAGTGACACAGGATCAGACAACGACTCAGAC
  AGTGATTCAAATAGCGATTCCGAGTCAGGTTCTAACAATAATGTAGTTCCGCCTAATTCA
  CCTAAAAATGGTACTAATGCTTCTAATAAAAATGAGGCTAAAGATAGTAAAGAACCATTA
  CCAGATACAGGTTCTGAAGATGAAGCGAATACGTCACTAATTTGGGGATTATTAGCATCA
  TTAGGTTCATTACTACTTTTCAGAAGAAAAAAAGAAAATAAAGATAAGAAATAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1380
  1440
  1500
  1560
  1620
  1680
  1740
  1800
  1860
  1920
  1980
  2040
  2100
  2160
  2220
  2280
  2340
  2400
  2460
  2520
  2580
  2640
  2700
  2760
  2820
  2880
  2934
  

⊟Protein

⊟General

• locus tag: JSNZ_000771
• symbol: ClfA
• description: MSCRAMM family adhesin clumping factor ClfA
• length: 977
• theoretical pI: 3.40051
• theoretical MW: 101251
• GRAVY: -1.08874

⊟Function

• TIGRFAM:
  gram-positive signal peptide, YSIRK family (TIGR01168; HMM-score: 28.2)
  Cell structure Cell envelope Other LPXTG cell wall anchor domain (TIGR01167; HMM-score: 27.2)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  Adhesin (CL0204) SdrG_C_C; C-terminus of bacterial fibrinogen-binding adhesin (PF10425; HMM-score: 148.4)
  and 3 more
  E-set (CL0159) Big_8; Bacterial Ig domain (PF17961; HMM-score: 91.6)
  no clan defined YSIRK_signal; YSIRK type signal peptide (PF04650; HMM-score: 36.3)
  Gram_pos_anchor; LPXTG cell wall anchor motif (PF00746; HMM-score: 22.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cellwall
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 10
  • Extracellular Score: 0
  • Internal Helices: 0
• DeepLocPro: Cell wall & surface
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0.0036
  • Cell wall & surface Score: 0.8462
  • Extracellular Score: 0.1502
• LocateP:
• SignalP: Signal peptide SP(Sec/SPI) length 39 aa
  • SP(Sec/SPI): 0.955818
  • TAT(Tat/SPI): 0.0097
  • LIPO(Sec/SPII): 0.030075
  • Cleavage Site: CS pos: 39-40. ADA-SE. Pr: 0.8960
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MNMKKKEKHAIRKKSIGVASVLVGTLIGFGLLSSKEADASENSVTQSDSASNESKSNDSSSINAAPKTDNTNVSDTKTTSNTNNGETSVAQNPAQQETTQSASTNATTEETPVTGETTTTATNQANTPATTQSSNTNAEELVNQTSNETTSNDTNTVSSVNSPQNSTNAENVSTTQDTSTEATPSNNESAPQSTDASNKDVVNQAVNTSAPRMRAFSLAAVAADAPAAGTDITNQLTDVKVTIDSGTTVYPHQAGYVKLNYGFSVPNSAVKGDTFKITVPKELNLNGVTSTAKVPPIMAGDQVLANGVIDSDGNVIYTFTDYVDNKENVTANITMPAYIDPENVTKTGNVTLTTGIGTNTASKTVLIDYEKYGQFHNLSIKGTIDQIDKTNNTYRQTIYVNPSGDNVVLPALTGNLIPNTKSNALIDAKNTDIKVYRVDNANDLSESYYVNPSDFEDVTNQVRISFPNANQYKVEFPTDDDQITTPYIVVVNGHIDPASTGDLALRSTFYGYDSNFIWRSMSWDNEVAFNNGSGSGDGIDKPVVPEQPDEPGEIEPIPEDSDSDPGSDSGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSESDSDSDSDSDSDSDSDSDSDSDSESDSDSDSDSDSESDSDSDSDSDSDSASDSDSGSDSDSSSDSDSDSTSDTGSDNDSDSDSNSDSESGSNNNVVPPNSPKNGTNASNKNEAKDSKEPLPDTGSEDEANTSLIWGLLASLGSLLLFRRKKENKDKK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for COL, NCTC8325, Newman

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

D McDevitt, P Francois, P Vaudaux, T J Foster
Molecular characterization of the clumping factor (fibrinogen receptor) of Staphylococcus aureus.
Mol Microbiol: 1994, 11(2);237-48
[PubMed:8170386] [WorldCat.org] [DOI] (P p)