PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_000694
• pan locus tag^?: SAUPAN002621000
• symbol: JSNZ_000694
• pan gene symbol^?: bmrU
• synonym:
• product: diacylglycerol kinase family protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_000694
• symbol: JSNZ_000694
• product: diacylglycerol kinase family protein
• replicon: chromosome
• strand: -
• coordinates: 730182..731099
• length: 918
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  
  ATGGAAAATAAATATACACATGGTGTCCTCTTTTACCATGAACATAGTGGATTAAAAAAT
  ATTAATCAAGGTATTGGGGAAGTTACAACAGCATTAAGTTCAATTTGTAAGCATCTTTCT
  ATTCAATTGAGTGAAAACGAAGGTGACATTATTAAATATTGCCAAGAAATCAAAACTAAA
  AACTATGCAAAAGATGTAGATATATTATTTATTCTTGGCGGTGATGGCACGGTTAACGAA
  CTTATAAATGGTGTTATGTCACATGACCTTCAACTCCCTATTGGCATTTTACCAGGCGGT
  ACTTTTAATGATTTTACAAAAACGCTAAATATAGCACCTAATCATAAACAAGCTAGTGAA
  CAAATGATTTCTGCACAAGTTGGTACATACGATGTAATTAAAATAAATAATCAATATGCA
  CTCAACTTTGTTGGGTTAGGTCTCATTGTTCAAAACGCTGAAAACGTACAAGATGGTTCA
  AAAGATATATTTGGTAAATTGAGTTATATTGGATCAACTGTCAAAACACTGCTAAATCCA
  ACACAATTTAATTATCAATTATCAATAGATGATAAGACATATTCTGGCGAAACGACCATG
  ATACTAACAGCAAATGGTCCATTCATTGGTGGTAGTCGTATACCATTGACTGATTTGTCT
  CCTCAAGATGGTGAACTGAACACTTTCATTTTTAATGAACAAAGTTTCAGTATTTTAAAT
  GATATTTTTAAAAAGCGTGATAGCATGAATTGGAATGAAATTACTCAAGGTATTGAGCAT
  ATACCTGGTAAAAAGATTTCACTTACAACAGATCCAGCAATGAAAGTTGATATTGATGGC
  GAAATTTCATTAGAAACACCTATTGATATTGAGGTTATACCAAATGCCATTCAGTTACTT
  ACTGTCAATGATTTGTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  918
  

⊟Protein

⊟General

• locus tag: JSNZ_000694
• symbol: JSNZ_000694
• description: diacylglycerol kinase family protein
• length: 305
• theoretical pI: 4.49354
• theoretical MW: 33611.8
• GRAVY: -0.171148

⊟Function

• reaction:
  EC 2.7.1.-?  ExPASy
• TIGRFAM:
  Unknown function Enzymes of unknown specificity lipid kinase, YegS/Rv2252/BmrU family (TIGR00147; EC 2.7.1.-; HMM-score: 169.1)
  and 1 more
  Unknown function Enzymes of unknown specificity lipid kinase YegS (TIGR03702; EC 2.7.1.-; HMM-score: 77.1)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  PFK (CL0240) DAGK_cat; Diacylglycerol kinase catalytic domain (PF00781; HMM-score: 98.4)
  and 2 more
  NAD_kinase_C (CL0701) YegS_C; YegS C-terminal NAD kinase beta sandwich-like domain (PF19279; HMM-score: 48.3)
  PBP (CL0177) SBP_bac_8; Bacterial extracellular solute-binding protein (PF13416; HMM-score: 12)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.848
  • Cytoplasmic Membrane Score: 0.1474
  • Cell wall & surface Score: 0.0002
  • Extracellular Score: 0.0044
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.016346
  • TAT(Tat/SPI): 0.00054
  • LIPO(Sec/SPII): 0.002097
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MENKYTHGVLFYHEHSGLKNINQGIGEVTTALSSICKHLSIQLSENEGDIIKYCQEIKTKNYAKDVDILFILGGDGTVNELINGVMSHDLQLPIGILPGGTFNDFTKTLNIAPNHKQASEQMISAQVGTYDVIKINNQYALNFVGLGLIVQNAENVQDGSKDIFGKLSYIGSTVKTLLNPTQFNYQLSIDDKTYSGETTMILTANGPFIGGSRIPLTDLSPQDGELNTFIFNEQSFSILNDIFKKRDSMNWNEITQGIEHIPGKKISLTTDPAMKVDIDGEISLETPIDIEVIPNAIQLLTVNDL

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: SigB (activation) regulon

  SigB

  (sigma factor)

  controlling a large regulon involved in stress/starvation response and adaptation; regulation predicted or transferred from N315 and NCTC 8325 in Wolfgramm et al. (https://doi.org/10.1101/2025.09.03.674026)   other strains

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications