PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_000478
• pan locus tag^?: SAUPAN002286000
• symbol: JSNZ_000478
• pan gene symbol^?: nupC
• synonym:
• product: NupC/NupG family nucleoside CNT transporter

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_000478
• symbol: JSNZ_000478
• product: NupC/NupG family nucleoside CNT transporter
• replicon: chromosome
• strand: -
• coordinates: 507281..508495
• length: 1215
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  
  ATGCATATTCTAATTGGGATTATCGGAATTATCTTCTTTTTAGCACTTGCGTTTCTATTT
  AGCTCAGACAGGAAAAATATTCGCTGGAAATATGTAGGAATTTTACTTGCAATTCAGCTT
  ATCTTTGCATTTATCTTGCTTAAAACTACAATGGGAATCAAGGTTATTGGTGGTATTTCA
  AACGGTTTTAACTACTTATTATTAAAAGCAGCAGATGGTGTTAACTTTGTATTCGGTGGT
  ATTCAATATATCGATCCTAAGCAACCACCATTCTTCTTCAGTGTATTATTACCTATCGTA
  TTTATTTCAGCGATTATCGGTATCCTTCAATACACAAAGATTTTACCTTTAATCATTAAT
  GTTTTAGGATTCTTAATTTCTAAAATTAATGGTATGGGACGTTTAGAATCATACAATGCA
  GTTGCTGCAGCAATTTTGGGACAATCTGAAGTATTTATTTCAATTAAAAAACAATTACCA
  TACATACCTAAGCAACGTTTATACACATTAACAGCTTCAGCAATGTCGACTGTTTCTGCT
  TCAATTATCGGTGCATACTTTACCTTAATTGAACCTAAATATGTTGTAACGGCTGTTGTC
  TTAAACTTATTTGGTGGTTTCATCATCGCTTCAATTATTAACCCTTATAAAGTTAATGAA
  GAAGATGACAAATTATTAGTTGAAGAAACTGAAGAAAGACAACAATCATTCTTCGAAATG
  CTAGGAGAATATATTTTAGATGGATTTAAAGTAGCTGTCATTGTAGGCGCAATGTTAATC
  GGATACATTGCTATTATCGCTCTATTAAATGGAATTGTAAGCAATATCTTTAGTACAGTT
  TCAGGTGGCGCTATTTCTTGGGATTTCCAAACATTAATTGGTTTTGTCTTTGCACCATTT
  GCATTCTTAGTCGGTGTGCCATGGCAAGATGCAGTTCAAGCCGGTTCAGTTATGGCGACA
  AAACTATTATCTAACGAATTTGTAGCAATGCAAGCACTTGGTAAACTTAGTGATTTATCT
  GAACACGCAAAAGGTGTTACATCTGTATTCTTAGTATCATTTGCTAACTTTAGCTCTATT
  GGTATTATTTCAGGTGCGATTAAATCACTTAATGACAAAAAAGGAGATACAGTTGCCCGC
  TTTGGTTTGAAACTATTATTCGGTGCAACACTAGTATCTTTCATTTCAGCTGCAATCGCT
  GGATTCTTTATTTAA
  60
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  360
  420
  480
  540
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  840
  900
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  1020
  1080
  1140
  1200
  1215
  

⊟Protein

⊟General

• locus tag: JSNZ_000478
• symbol: JSNZ_000478
• description: NupC/NupG family nucleoside CNT transporter
• length: 404
• theoretical pI: 9.44764
• theoretical MW: 43804.7
• GRAVY: 0.870792

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Nucleosides, purines and pyrimidines nucleoside transporter, NupC family (TIGR00804; HMM-score: 433.4)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  no clan defined Nucleos_tra2_C; Na+ dependent nucleoside transporter C-terminus (PF07662; HMM-score: 214.4)
  and 2 more
  Nucleos_tra2_N; Na+ dependent nucleoside transporter N-terminus (PF01773; HMM-score: 79.4)
  Gate; Nucleoside recognition (PF07670; HMM-score: 50)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 10
• DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0.9997
  • Cell wall & surface Score: 0
  • Extracellular Score: 0.0003
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.011503
  • TAT(Tat/SPI): 0.000267
  • LIPO(Sec/SPII): 0.060914
• predicted transmembrane helices (TMHMM): 10

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MHILIGIIGIIFFLALAFLFSSDRKNIRWKYVGILLAIQLIFAFILLKTTMGIKVIGGISNGFNYLLLKAADGVNFVFGGIQYIDPKQPPFFFSVLLPIVFISAIIGILQYTKILPLIINVLGFLISKINGMGRLESYNAVAAAILGQSEVFISIKKQLPYIPKQRLYTLTASAMSTVSASIIGAYFTLIEPKYVVTAVVLNLFGGFIIASIINPYKVNEEDDKLLVEETEERQQSFFEMLGEYILDGFKVAVIVGAMLIGYIAIIALLNGIVSNIFSTVSGGAISWDFQTLIGFVFAPFAFLVGVPWQDAVQAGSVMATKLLSNEFVAMQALGKLSDLSEHAKGVTSVFLVSFANFSSIGIISGAIKSLNDKKGDTVARFGLKLLFGATLVSFISAAIAGFFI

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulators: CtsR* (repression) regulon, CcpA regulon

  CtsR*	(TF)	important in Heat shock response; regulation predicted or transferred from N315 and NCTC 8325 in Wolfgramm et al. (https://doi.org/10.1101/2025.09.03.674026)
  CcpA	(TF)	important in Carbon catabolism; regulation predicted or transferred from N315 and NCTC 8325 in Wolfgramm et al. (https://doi.org/10.1101/2025.09.03.674026)

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You can add further information about the gene and protein here.

⊟Literature

⊟References

⊟Relevant publications