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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_000378
  • pan locus tag?: SAUPAN002155000
  • symbol: JSNZ_000378
  • pan gene symbol?: zagA
  • synonym:
  • product: GTP-binding protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_000378
  • symbol: JSNZ_000378
  • product: GTP-binding protein
  • replicon: chromosome
  • strand: +
  • coordinates: 409859..411061
  • length: 1203
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGGCTAAGATTCCAGTTACGGTATTAAGCGGTTATTTAGGCTCGGGGAAAACTACGTTG
    TTAAATCATATTTTACAAAATCGTGAAGGTCGACGTATTGCGGTAATTGTAAATGATATG
    AGTGAAGTCAATATCGATAAAGATCTTGTCGCAGATGGTGGGGGACTATCGCGTACAGAT
    GAAAAATTAGTCGAACTCTCTAATGGTTGTATCTGTTGTACACTTAGAGATGATTTATTA
    AAAGAAGTTGAGCGTTTAGTGAAAAAAGGTGGCATCGATCAAATTGTTATTGAGTCAACA
    GGGATTTCAGAGCCAGTACCTGTTGCACAAACTTTCTCATATATTGATGATGAACTTGGC
    ATTGATCTTACAGCGATTTGCCGTTTAGATACAATGGTTACAGTTGTTGATGCTAACCGC
    TTCGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGATGAA
    ACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTG
    ATTATTAATAAAATTGATTTAATTAGTGAAGAAGAACTAGCGAAGTTAGAAAAAGTGTTA
    AGTGCATTGCAACCGACTGCTAAAATTATTAAGACAACAAATTCTGAAGTAGATTTAAAA
    GAAGTTTTAAATACGCAGCGTTTTGATTTTGAAAAAGCGAGTGAGTCAGCAGGATGGATC
    AAAGAACTTGAGTCTGGTGGGCATGCATCGCATACACCTGAAACAGAAGAATATGGTATA
    TCATCGTTTGTATATAAACGTCGACTTCCTTTCCATGCTAAAAGGTTCAATGATTGGTTA
    GAAACCATGCCAAATAATGTCGTTCGATCAAAAGGTATCGTATGGCTAGCACAATACAAT
    CACGTAGCATGTTTATTATCTCAAGCAGGGTCATCTTGCAATATTCATCCAGTTACATAT
    TGGGTGGCTAGTATGTCTGAAGCGCAACAAACGCAAATATTAGCAGAACGCCAAGACGTC
    GCAGCTGAATGGGATCCAGAATATGGCGATCGTCATACACAATTTGTCATTATTGGTACC
    GACTTAGATGAAGAAAAATTAACAAAAGAACTCGATGCATGCTTAGTCAATGCGCAAGAA
    ATTGATGCAGATTGGCAACAATTTGAAGATCCATATCAATGGCAAATTAGACCAGCACGA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1203

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_000378
  • symbol: JSNZ_000378
  • description: GTP-binding protein
  • length: 400
  • theoretical pI: 4.30024
  • theoretical MW: 45015.4
  • GRAVY: -0.2775

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin cobalamin biosynthesis protein CobW (TIGR02475; HMM-score: 157.5)
    and 14 more
    Genetic information processing Protein fate Protein modification and repair hydrogenase accessory protein HypB (TIGR00073; HMM-score: 28.8)
    helicase/secretion neighborhood ATPase (TIGR03819; HMM-score: 19.7)
    Unknown function General small GTP-binding protein domain (TIGR00231; HMM-score: 17)
    Metabolism Central intermediary metabolism Nitrogen metabolism urease accessory protein UreG (TIGR00101; HMM-score: 15.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Molybdopterin molybdopterin-guanine dinucleotide biosynthesis protein B (TIGR00176; HMM-score: 15.2)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking putative secretion ATPase, PEP-CTERM locus subfamily (TIGR03015; HMM-score: 14.1)
    Cellular processes Cellular processes Toxin production and resistance putative bacteriocin export ABC transporter, lactococcin 972 group (TIGR03608; HMM-score: 13.9)
    Metabolism Transport and binding proteins Unknown substrate putative bacteriocin export ABC transporter, lactococcin 972 group (TIGR03608; HMM-score: 13.9)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA threonylcarbamoyl adenosine modification protein YjeE (TIGR00150; HMM-score: 13.5)
    Genetic information processing Protein synthesis Other ribosome-associated GTPase EngA (TIGR03594; HMM-score: 13.4)
    P-type DNA transfer ATPase VirB11 (TIGR02788; HMM-score: 13.2)
    Metabolism Transport and binding proteins Amino acids, peptides and amines LAO/AO transport system ATPase (TIGR00750; EC 2.7.-.-; HMM-score: 11.3)
    Signal transduction Regulatory functions Protein interactions LAO/AO transport system ATPase (TIGR00750; EC 2.7.-.-; HMM-score: 11.3)
    Unknown function General GTP-binding protein HflX (TIGR03156; HMM-score: 11)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    P-loop_NTPase (CL0023) cobW; CobW/HypB/UreG, nucleotide-binding domain (PF02492; HMM-score: 198.4)
    and 23 more
    CobW_C (CL0832) CobW_C; Cobalamin synthesis protein cobW C-terminal domain (PF07683; HMM-score: 86.3)
    P-loop_NTPase (CL0023) RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 23.8)
    PspA (CL0235) Snf7; Snf7 (PF03357; HMM-score: 20.9)
    P-loop_NTPase (CL0023) GTP_EFTU; Elongation factor Tu GTP binding domain (PF00009; HMM-score: 19.9)
    no clan defined MMR_HSR1_Xtn; C-terminal region of MMR_HSR1 domain (PF16897; HMM-score: 16.8)
    P-loop_NTPase (CL0023) AAA_16; AAA ATPase domain (PF13191; HMM-score: 16.1)
    ATPase_2; ATPase domain predominantly from Archaea (PF01637; HMM-score: 15.9)
    MobB; Molybdopterin guanine dinucleotide synthesis protein B (PF03205; HMM-score: 15.3)
    AAA_22; AAA domain (PF13401; HMM-score: 15.1)
    ABC_tran; ABC transporter (PF00005; HMM-score: 14.7)
    TsaE; Threonylcarbamoyl adenosine biosynthesis protein TsaE (PF02367; HMM-score: 14.2)
    AAA_23; AAA domain (PF13476; HMM-score: 14.1)
    T2SSE; Type II/IV secretion system protein (PF00437; HMM-score: 13.3)
    AAA_5; AAA domain (dynein-related subfamily) (PF07728; HMM-score: 13.3)
    AAA_14; AAA domain (PF13173; HMM-score: 13.1)
    NACHT; NACHT domain (PF05729; HMM-score: 13)
    TrwB_AAD_bind; Type IV secretion-system coupling protein DNA-binding domain (PF10412; HMM-score: 12.9)
    AAA_18; AAA domain (PF13238; HMM-score: 12.9)
    MeaB; Methylmalonyl Co-A mutase-associated GTPase MeaB (PF03308; HMM-score: 12.6)
    nSTAND3; Novel STAND NTPase 3 (PF20720; HMM-score: 12.5)
    AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 12.4)
    AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 11.5)
    MMR_HSR1; 50S ribosome-binding GTPase (PF01926; HMM-score: 11)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9528
    • Cytoplasmic Membrane Score: 0.0152
    • Cell wall & surface Score: 0.0001
    • Extracellular Score: 0.0318
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.008153
    • TAT(Tat/SPI): 0.000308
    • LIPO(Sec/SPII): 0.002284
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MAKIPVTVLSGYLGSGKTTLLNHILQNREGRRIAVIVNDMSEVNIDKDLVADGGGLSRTDEKLVELSNGCICCTLRDDLLKEVERLVKKGGIDQIVIESTGISEPVPVAQTFSYIDDELGIDLTAICRLDTMVTVVDANRFVHDINSEDLLMDRDQSVDETDERSIADLLIDQVEFCDVLIINKIDLISEEELAKLEKVLSALQPTAKIIKTTNSEVDLKEVLNTQRFDFEKASESAGWIKELESGGHASHTPETEEYGISSFVYKRRLPFHAKRFNDWLETMPNNVVRSKGIVWLAQYNHVACLLSQAGSSCNIHPVTYWVASMSEAQQTQILAERQDVAAEWDPEYGDRHTQFVIIGTDLDEEKLTKELDACLVNAQEIDADWQQFEDPYQWQIRPAR

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: Zur* (repression) regulon
    Zur*(TF)important in Zinc homeostasis;  regulation predicted or transferred from N315 and NCTC 8325  [1]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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