PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_000378
• pan locus tag^?: SAUPAN002155000
• symbol: JSNZ_000378
• pan gene symbol^?: zagA
• synonym:
• product: GTP-binding protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_000378
• symbol: JSNZ_000378
• product: GTP-binding protein
• replicon: chromosome
• strand: +
• coordinates: 409859..411061
• length: 1203
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  
  ATGGCTAAGATTCCAGTTACGGTATTAAGCGGTTATTTAGGCTCGGGGAAAACTACGTTG
  TTAAATCATATTTTACAAAATCGTGAAGGTCGACGTATTGCGGTAATTGTAAATGATATG
  AGTGAAGTCAATATCGATAAAGATCTTGTCGCAGATGGTGGGGGACTATCGCGTACAGAT
  GAAAAATTAGTCGAACTCTCTAATGGTTGTATCTGTTGTACACTTAGAGATGATTTATTA
  AAAGAAGTTGAGCGTTTAGTGAAAAAAGGTGGCATCGATCAAATTGTTATTGAGTCAACA
  GGGATTTCAGAGCCAGTACCTGTTGCACAAACTTTCTCATATATTGATGATGAACTTGGC
  ATTGATCTTACAGCGATTTGCCGTTTAGATACAATGGTTACAGTTGTTGATGCTAACCGC
  TTCGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGATGAA
  ACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTG
  ATTATTAATAAAATTGATTTAATTAGTGAAGAAGAACTAGCGAAGTTAGAAAAAGTGTTA
  AGTGCATTGCAACCGACTGCTAAAATTATTAAGACAACAAATTCTGAAGTAGATTTAAAA
  GAAGTTTTAAATACGCAGCGTTTTGATTTTGAAAAAGCGAGTGAGTCAGCAGGATGGATC
  AAAGAACTTGAGTCTGGTGGGCATGCATCGCATACACCTGAAACAGAAGAATATGGTATA
  TCATCGTTTGTATATAAACGTCGACTTCCTTTCCATGCTAAAAGGTTCAATGATTGGTTA
  GAAACCATGCCAAATAATGTCGTTCGATCAAAAGGTATCGTATGGCTAGCACAATACAAT
  CACGTAGCATGTTTATTATCTCAAGCAGGGTCATCTTGCAATATTCATCCAGTTACATAT
  TGGGTGGCTAGTATGTCTGAAGCGCAACAAACGCAAATATTAGCAGAACGCCAAGACGTC
  GCAGCTGAATGGGATCCAGAATATGGCGATCGTCATACACAATTTGTCATTATTGGTACC
  GACTTAGATGAAGAAAAATTAACAAAAGAACTCGATGCATGCTTAGTCAATGCGCAAGAA
  ATTGATGCAGATTGGCAACAATTTGAAGATCCATATCAATGGCAAATTAGACCAGCACGA
  TAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1203
  

⊟Protein

⊟General

• locus tag: JSNZ_000378
• symbol: JSNZ_000378
• description: GTP-binding protein
• length: 400
• theoretical pI: 4.30024
• theoretical MW: 45015.4
• GRAVY: -0.2775

⊟Function

• TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin cobalamin biosynthesis protein CobW (TIGR02475; HMM-score: 157.5)
  and 14 more
  Genetic information processing Protein fate Protein modification and repair hydrogenase accessory protein HypB (TIGR00073; HMM-score: 28.8)
  helicase/secretion neighborhood ATPase (TIGR03819; HMM-score: 19.7)
  Unknown function General small GTP-binding protein domain (TIGR00231; HMM-score: 17)
  Metabolism Central intermediary metabolism Nitrogen metabolism urease accessory protein UreG (TIGR00101; HMM-score: 15.8)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Molybdopterin molybdopterin-guanine dinucleotide biosynthesis protein B (TIGR00176; HMM-score: 15.2)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking putative secretion ATPase, PEP-CTERM locus subfamily (TIGR03015; HMM-score: 14.1)
  Cellular processes Cellular processes Toxin production and resistance putative bacteriocin export ABC transporter, lactococcin 972 group (TIGR03608; HMM-score: 13.9)
  Metabolism Transport and binding proteins Unknown substrate putative bacteriocin export ABC transporter, lactococcin 972 group (TIGR03608; HMM-score: 13.9)
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA threonylcarbamoyl adenosine modification protein YjeE (TIGR00150; HMM-score: 13.5)
  Genetic information processing Protein synthesis Other ribosome-associated GTPase EngA (TIGR03594; HMM-score: 13.4)
  P-type DNA transfer ATPase VirB11 (TIGR02788; HMM-score: 13.2)
  Metabolism Transport and binding proteins Amino acids, peptides and amines LAO/AO transport system ATPase (TIGR00750; EC 2.7.-.-; HMM-score: 11.3)
  Signal transduction Regulatory functions Protein interactions LAO/AO transport system ATPase (TIGR00750; EC 2.7.-.-; HMM-score: 11.3)
  Unknown function General GTP-binding protein HflX (TIGR03156; HMM-score: 11)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  P-loop_NTPase (CL0023) cobW; CobW/HypB/UreG, nucleotide-binding domain (PF02492; HMM-score: 198.4)
  and 23 more
  CobW_C (CL0832) CobW_C; Cobalamin synthesis protein cobW C-terminal domain (PF07683; HMM-score: 86.3)
  P-loop_NTPase (CL0023) RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 23.8)
  PspA (CL0235) Snf7; Snf7 (PF03357; HMM-score: 20.9)
  P-loop_NTPase (CL0023) GTP_EFTU; Elongation factor Tu GTP binding domain (PF00009; HMM-score: 19.9)
  no clan defined MMR_HSR1_Xtn; C-terminal region of MMR_HSR1 domain (PF16897; HMM-score: 16.8)
  P-loop_NTPase (CL0023) AAA_16; AAA ATPase domain (PF13191; HMM-score: 16.1)
  ATPase_2; ATPase domain predominantly from Archaea (PF01637; HMM-score: 15.9)
  MobB; Molybdopterin guanine dinucleotide synthesis protein B (PF03205; HMM-score: 15.3)
  AAA_22; AAA domain (PF13401; HMM-score: 15.1)
  ABC_tran; ABC transporter (PF00005; HMM-score: 14.7)
  TsaE; Threonylcarbamoyl adenosine biosynthesis protein TsaE (PF02367; HMM-score: 14.2)
  AAA_23; AAA domain (PF13476; HMM-score: 14.1)
  T2SSE; Type II/IV secretion system protein (PF00437; HMM-score: 13.3)
  AAA_5; AAA domain (dynein-related subfamily) (PF07728; HMM-score: 13.3)
  AAA_14; AAA domain (PF13173; HMM-score: 13.1)
  NACHT; NACHT domain (PF05729; HMM-score: 13)
  TrwB_AAD_bind; Type IV secretion-system coupling protein DNA-binding domain (PF10412; HMM-score: 12.9)
  AAA_18; AAA domain (PF13238; HMM-score: 12.9)
  MeaB; Methylmalonyl Co-A mutase-associated GTPase MeaB (PF03308; HMM-score: 12.6)
  nSTAND3; Novel STAND NTPase 3 (PF20720; HMM-score: 12.5)
  AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 12.4)
  AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 11.5)
  MMR_HSR1; 50S ribosome-binding GTPase (PF01926; HMM-score: 11)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9528
  • Cytoplasmic Membrane Score: 0.0152
  • Cell wall & surface Score: 0.0001
  • Extracellular Score: 0.0318
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.008153
  • TAT(Tat/SPI): 0.000308
  • LIPO(Sec/SPII): 0.002284
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MAKIPVTVLSGYLGSGKTTLLNHILQNREGRRIAVIVNDMSEVNIDKDLVADGGGLSRTDEKLVELSNGCICCTLRDDLLKEVERLVKKGGIDQIVIESTGISEPVPVAQTFSYIDDELGIDLTAICRLDTMVTVVDANRFVHDINSEDLLMDRDQSVDETDERSIADLLIDQVEFCDVLIINKIDLISEEELAKLEKVLSALQPTAKIIKTTNSEVDLKEVLNTQRFDFEKASESAGWIKELESGGHASHTPETEEYGISSFVYKRRLPFHAKRFNDWLETMPNNVVRSKGIVWLAQYNHVACLLSQAGSSCNIHPVTYWVASMSEAQQTQILAERQDVAAEWDPEYGDRHTQFVIIGTDLDEEKLTKELDACLVNAQEIDADWQQFEDPYQWQIRPAR

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: Zur* (repression) regulon

  Zur*

  (TF)

  important in Zinc homeostasis; transcription unit predicted or transferred from N315 and NCTC 8325 in Wolfgramm et al. (https://doi.org/10.1101/2025.09.03.674026)

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications