PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_000346
• pan locus tag^?: SAUPAN001997000
• symbol: guaA
• pan gene symbol^?: guaA
• synonym:
• product: glutamine-hydrolyzing GMP synthase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_000346
• symbol: guaA
• product: glutamine-hydrolyzing GMP synthase
• replicon: chromosome
• strand: +
• coordinates: 379368..380909
• length: 1542
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  1501
  
  ATGGAAATGGCAAAAGAACAAGAGTTAATCCTTGTCTTAGACTTTGGTAGCCAATACAAC
  CAATTAATTACACGCCGAATTCGTGAAATGGGCGTTTATAGTGAATTACACGATCATGAA
  ATTTCAATTGAAGAAATTAAGAAAATGAATCCAAAAGGTATTATCTTATCAGGTGGTCCA
  AATTCAGTTTATGAAGAAGGTTCATTTACAATTGATCCGGAAATATATAATTTAGGAATT
  CCAGTACTTGGTATTTGTTACGGCATGCAATTAACTACTAAATTATTAGGTGGTAAAGTT
  GAACGTGCCAATGAACGTGAATACGGTAAAGCAATCATTAATGCGAAGTCAGATGAGTTA
  TTCGCTGGCTTACCAGCAGAACAAACTGTTTGGATGAGTCATTCTGATAAAGTTATTGAA
  ATTCCAGAAGGCTTTGAAGTTATCGCTGATAGCCCAAGCACAGACTATGCAGCAATCGAA
  GATAAGAAACGTCGCATTTATGGTGTGCAATTCCATCCAGAAGTACGTCATACAGAATAT
  GGTAATGATTTATTAAATAATTTTGTCCGTCGTGTTTGTGATTGTAAAGGTCAATGGACA
  ATGGAAAACTTTATCGAAATCGAAATTGAAAAGATTCGTCAACGAGTAGGAGACCGTCGT
  GTATTATGTGCGATGAGTGGCGGCGTAGATTCATCTGTTGTTGCTGTATTATTGCATAAA
  GCAATAGGTGATCAACTAACATGTATCTTTGTAGACCATGGCTTACTTCGTAAAGGTGAA
  GGCGACATGGTTATGGAACAATTCGGTGAAGGGTTCAACATGAATATTATTCGTGTTAAT
  GCGAAAGATCGTTTTATGAATAAATTAAAAGGTGTTTCAGATCCTGAACAAAAACGTAAA
  ATCATTGGTAATGAATTTGTATATGTATTTGATGATGAAGCATCAAAACTGAAAGGTGTA
  GACTTCCTTGCGCAAGGAACACTTTATACAGACGTTATCGAATCAGGTACCAAGACAGCA
  CAAACAATCAAATCACATCACAATGTTGGTGGATTACCAGAAGACATGGAATTCGAATTA
  ATCGAACCAATCAATACATTGTTTAAAGATGAGGTACGTAAATTAGGTATTGAATTAGGT
  ATTCCAGAACATTTAGTATGGAGACAACCATTCCCAGGACCTGGTCTTGGTATTCGTGTA
  CTTGGAGAAATTACTGAAGATAAACTAGAAATCGTTAGAGAATCAGACGCAATTTTACGC
  CAAGTGATTAGAGAAGAAGGTCTTGAAAGAGAAATTTGGCAATACTTCACAGTGTTACCA
  AACATTCAATCAGTAGGTGTTATGGGAGACTACCGTACGTATGATCACACAGTAGGTATC
  CGTGCAGTAACATCTATCGACGGCATGACAAGTGATTTTGCACGCATCGATTGGGAAGTC
  TTACAAAAGATTTCAAGTCGTATCGTAAACGAAGTAGACCACGTTAACCGCGTAGTCTAT
  GACATTACATCAAAACCACCAAGCACAATTGAGTGGGAATAA
  60
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  240
  300
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  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1380
  1440
  1500
  1542
  

⊟Protein

⊟General

• locus tag: JSNZ_000346
• symbol: GuaA
• description: glutamine-hydrolyzing GMP synthase
• length: 513
• theoretical pI: 4.76247
• theoretical MW: 58202
• GRAVY: -0.288109

⊟Function

• reaction:
  EC 6.3.5.2?  ExPASy
  GMP synthase (glutamine-hydrolyzing) ATP + XMP + L-glutamine + H₂O = AMP + diphosphate + GMP + L-glutamate
• TIGRFAM:
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis GMP synthase (glutamine-hydrolyzing), C-terminal domain (TIGR00884; EC 6.3.5.2; HMM-score: 508)
  and 13 more
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis GMP synthase (glutamine-hydrolyzing), N-terminal domain (TIGR00888; EC 6.3.5.2; HMM-score: 275.1)
  glutamine amidotransferase of anthranilate synthase or aminodeoxychorismate synthase (TIGR00566; HMM-score: 65.1)
  Metabolism Amino acid biosynthesis Aromatic amino acid family anthranilate synthase (TIGR01815; EC 4.1.3.27; HMM-score: 56.3)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis carbamoyl-phosphate synthase, small subunit (TIGR01368; EC 6.3.5.5; HMM-score: 52.7)
  Metabolism Amino acid biosynthesis Histidine family imidazole glycerol phosphate synthase, glutamine amidotransferase subunit (TIGR01855; EC 2.4.2.-; HMM-score: 35.8)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridine nucleotides NAD+ synthetase (TIGR00552; EC 6.3.1.5; HMM-score: 35.6)
  aminodeoxychorismate synthase (TIGR01823; EC 2.6.1.85; HMM-score: 32.1)
  Hypothetical proteins Conserved TIGR00268 family protein (TIGR00268; HMM-score: 24.3)
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase (TIGR00420; EC 2.1.1.61; HMM-score: 20.4)
  Metabolism Amino acid biosynthesis Aspartate family asparagine synthase (glutamine-hydrolyzing) (TIGR01536; EC 6.3.5.4; HMM-score: 17.7)
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA(Ile)-lysidine synthetase (TIGR02432; EC 6.3.4.-; HMM-score: 17.6)
  asparagine synthase family amidotransferase (TIGR03104; HMM-score: 14.7)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis CTP synthase (TIGR00337; EC 6.3.4.2; HMM-score: 12.2)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  Glutaminase_I (CL0014) GATase; Glutamine amidotransferase class-I (PF00117; HMM-score: 174.3)
  no clan defined GMP_synt_C; GMP synthase C terminal domain (PF00958; HMM-score: 147.8)
  and 9 more
  Glutaminase_I (CL0014) Peptidase_C26; Peptidase C26 (PF07722; HMM-score: 38)
  HUP (CL0039) NAD_synthase; NAD synthase (PF02540; HMM-score: 37)
  tRNA_Me_trans; tRNA methyl transferase HUP domain (PF03054; HMM-score: 25.9)
  Asn_synthase; Asparagine synthase (PF00733; HMM-score: 21.3)
  ATP_bind_3; PP-loop family (PF01171; HMM-score: 20.2)
  QueC; Queuosine biosynthesis protein QueC (PF06508; HMM-score: 18.1)
  Arginosuc_synth; Arginosuccinate synthase N-terminal HUP domain (PF00764; HMM-score: 14.9)
  PAPS_reduct; Phosphoadenosine phosphosulfate reductase family (PF01507; HMM-score: 14.9)
  no clan defined LEA_6; Late embryogenesis abundant protein 18 (PF10714; HMM-score: 12.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9107
  • Cytoplasmic Membrane Score: 0.0295
  • Cell wall & surface Score: 0.0008
  • Extracellular Score: 0.059
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.00385
  • TAT(Tat/SPI): 0.00072
  • LIPO(Sec/SPII): 0.000338
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MEMAKEQELILVLDFGSQYNQLITRRIREMGVYSELHDHEISIEEIKKMNPKGIILSGGPNSVYEEGSFTIDPEIYNLGIPVLGICYGMQLTTKLLGGKVERANEREYGKAIINAKSDELFAGLPAEQTVWMSHSDKVIEIPEGFEVIADSPSTDYAAIEDKKRRIYGVQFHPEVRHTEYGNDLLNNFVRRVCDCKGQWTMENFIEIEIEKIRQRVGDRRVLCAMSGGVDSSVVAVLLHKAIGDQLTCIFVDHGLLRKGEGDMVMEQFGEGFNMNIIRVNAKDRFMNKLKGVSDPEQKRKIIGNEFVYVFDDEASKLKGVDFLAQGTLYTDVIESGTKTAQTIKSHHNVGGLPEDMEFELIEPINTLFKDEVRKLGIELGIPEHLVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILRQVIREEGLEREIWQYFTVLPNIQSVGVMGDYRTYDHTVGIRAVTSIDGMTSDFARIDWEVLQKISSRIVNEVDHVNRVVYDITSKPPSTIEWE

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for N315

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications