PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_000345
• pan locus tag^?: SAUPAN001996000
• symbol: guaB
• pan gene symbol^?: guaB
• synonym:
• product: IMP dehydrogenase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_000345
• symbol: guaB
• product: IMP dehydrogenase
• replicon: chromosome
• strand: +
• coordinates: 377877..379343
• length: 1467
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  
  ATGTGGGAAAGTAAATTTGCAAAAGAATCATTAACGTTTGATGATGTGTTATTAATTCCA
  GCACAATCTGATATTTTACCGAAAGACGTTGATTTAAGCGTACAATTATCAGACAAAGTT
  AAATTGAATATTCCAGTTATTTCTGCTGGTATGGATACAGTAACTGAATCTAAAATGGCG
  ATTGCTATGGCTCGTCAAGGTGGTTTAGGTGTTATTCATAAAAATATGGGCGTTGAAGAA
  CAAGCGGACGAAGTTCAAAAAGTAAAACGCTCAGAAAATGGTGTCATTTCAAACCCATTT
  TTCTTAACGCCAGAAGAAAGCGTTTATGAAGCAGAAGCATTAATGGGTAAATACCGTATT
  TCAGGTGTACCAATTGTTGATAATAAAGAAGATCGCAACTTAGTAGGTATTTTAACAAAC
  CGCGACTTACGTTTTATTGAAGACTTCTCAATTAAAATTGTAGATGTAATGACGCAAGAA
  AATTTAATTACAGCTCCAGTGAATACAACACTTGAAGAAGCAGAAAAAATTCTCCAAAAA
  CATAAGATTGAAAAGTTACCATTAGTTAAAGACGGACGTCTAGAAGGTCTTATTACTATT
  AAAGATATTGAAAAAGTAATCGAATTCCCTAATGCAGCAAAAGATGAACATGGTCGTCTA
  CTTGTAGCCGCAGCAATCGGTATTTCAAAAGACACTGATATTCGTGCTCAAAAATTAGTC
  GAAGCAGGTGTGGATGTCTTAGTTATCGATACAGCACATGGTCACTCTAAAGGCGTTATC
  GATCAAGTGAAACATATTAAGAAGACTTACCCAGAAATCACATTAGTAGCTGGTAACGTA
  GCAACTGCAGAAGCAACAAAAGATTTATTTGAAGCGGGTGCAGATATTGTTAAAGTTGGT
  ATTGGCCCAGGTTCAATTTGTACGACGCGTGTTGTAGCAGGTGTTGGTGTACCACAAATT
  ACAGCAATTTATGATTGTGCAACTGAAGCACGCAAACATGGTAAAGCTATCATTGCTGAT
  GGCGGTATTAAATTCTCAGGAGATATCATTAAAGCATTAGCTGCTGGTGGACATGCGGTT
  ATGTTAGGTAGCTTATTAGCAGGTACTGAAGAAAGTCCAGGCGCAACAGAAATTTTCCAA
  GGTAGACAATATAAAGTATACCGTGGTATGGGCTCTTTAGGTGCGATGGAAAAAGGTTCA
  AACGACCGTTACTTCCAAGAGGACAAAGCGCCTAAGAAATTTGTTCCTGAAGGTATCGAA
  GGACGTACGGCATATAAAGGTGCTTTACAAGATACAATTTACCAATTAATGGGCGGAGTG
  CGTGCTGGTATGGGTTATACTGGTTCACACGATTTAAGAGAATTACGCGAAGAAGCACAA
  TTCACACGTATGGGTCCTGCTGGTTTAGCAGAAAGCCACCCACATAATATTCAAATTACG
  AAAGAATCACCGAACTACTCATTCTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1380
  1440
  1467
  

⊟Protein

⊟General

• locus tag: JSNZ_000345
• symbol: GuaB
• description: IMP dehydrogenase
• length: 488
• theoretical pI: 5.6874
• theoretical MW: 52850.2
• GRAVY: -0.172541

⊟Function

• reaction:
  EC 1.1.1.205?  ExPASy
  IMP dehydrogenase Inosine 5'-phosphate + NAD⁺ + H₂O = xanthosine 5'-phosphate + NADH
• TIGRFAM:
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis inosine-5'-monophosphate dehydrogenase (TIGR01302; EC 1.1.1.205; HMM-score: 660.9)
  and 13 more
  Unknown function General IMP dehydrogenase family protein (TIGR01303; HMM-score: 346.9)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Nucleotide and nucleoside interconversions guanosine monophosphate reductase (TIGR01305; EC 1.7.1.7; HMM-score: 202)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Nucleotide and nucleoside interconversions guanosine monophosphate reductase (TIGR01306; EC 1.7.1.7; HMM-score: 177.7)
  Unknown function General IMP dehydrogenase family protein (TIGR01304; HMM-score: 123.2)
  Metabolism Energy metabolism Sugars sugar isomerase, KpsF/GutQ family (TIGR00393; HMM-score: 22.1)
  putative enoyl-[acyl-carrier-protein] reductase II (TIGR03151; EC 1.3.1.-; HMM-score: 22)
  Metabolism Transport and binding proteins Amino acids, peptides and amines glycine betaine/L-proline transport ATP binding subunit (TIGR01186; HMM-score: 19.5)
  heme/flavin dehydrogenase, mycofactocin system (TIGR03966; EC 1.-.-.-; HMM-score: 18.1)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other isopentenyl-diphosphate delta-isomerase, type 2 (TIGR02151; EC 5.3.3.2; HMM-score: 17.9)
  Metabolism Amino acid biosynthesis Glutamate family 2-dehydro-3-deoxyphosphogluconate aldolase/4-hydroxy-2-oxoglutarate aldolase (TIGR01182; EC 4.1.2.14,4.1.3.16; HMM-score: 17.5)
  Metabolism Energy metabolism Entner-Doudoroff 2-dehydro-3-deoxyphosphogluconate aldolase/4-hydroxy-2-oxoglutarate aldolase (TIGR01182; EC 4.1.2.14,4.1.3.16; HMM-score: 17.5)
  Metabolism Energy metabolism Sugars UDP-glucose 4-epimerase GalE (TIGR01179; EC 5.1.3.2; HMM-score: 14.4)
  geranylgeranylglyceryl phosphate synthase family protein (TIGR01768; EC 2.5.1.-; HMM-score: 9.7)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  TIM_barrel (CL0036) IMPDH; IMP dehydrogenase / GMP reductase domain (PF00478; HMM-score: 548.8)
  and 7 more
  no clan defined CBS; CBS domain (PF00571; HMM-score: 80.6)
  TIM_barrel (CL0036) NMO; Nitronate monooxygenase (PF03060; HMM-score: 37.1)
  FMN_dh; FMN-dependent dehydrogenase (PF01070; HMM-score: 20.1)
  Aldolase; KDPG and KHG aldolase (PF01081; HMM-score: 17)
  Glu_synthase; Conserved region in glutamate synthase (PF01645; HMM-score: 15.4)
  NanE; Putative N-acetylmannosamine-6-phosphate epimerase (PF04131; HMM-score: 13.4)
  Phosphatase (CL0031) DSPc; Dual specificity phosphatase, catalytic domain (PF00782; HMM-score: 12.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.757
  • Cytoplasmic Membrane Score: 0.0226
  • Cell wall & surface Score: 0.0007
  • Extracellular Score: 0.2197
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004372
  • TAT(Tat/SPI): 0.000531
  • LIPO(Sec/SPII): 0.000662
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MWESKFAKESLTFDDVLLIPAQSDILPKDVDLSVQLSDKVKLNIPVISAGMDTVTESKMAIAMARQGGLGVIHKNMGVEEQADEVQKVKRSENGVISNPFFLTPEESVYEAEALMGKYRISGVPIVDNKEDRNLVGILTNRDLRFIEDFSIKIVDVMTQENLITAPVNTTLEEAEKILQKHKIEKLPLVKDGRLEGLITIKDIEKVIEFPNAAKDEHGRLLVAAAIGISKDTDIRAQKLVEAGVDVLVIDTAHGHSKGVIDQVKHIKKTYPEITLVAGNVATAEATKDLFEAGADIVKVGIGPGSICTTRVVAGVGVPQITAIYDCATEARKHGKAIIADGGIKFSGDIIKALAAGGHAVMLGSLLAGTEESPGATEIFQGRQYKVYRGMGSLGAMEKGSNDRYFQEDKAPKKFVPEGIEGRTAYKGALQDTIYQLMGGVRAGMGYTGSHDLRELREEAQFTRMGPAGLAESHPHNIQITKESPNYSF

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for N315

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications