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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_000314
  • pan locus tag?: SAUPAN001906000
  • symbol: JSNZ_000314
  • pan gene symbol?: parB
  • synonym:
  • product: ParB/RepB/Spo0J family partition protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_000314
  • symbol: JSNZ_000314
  • product: ParB/RepB/Spo0J family partition protein
  • replicon: chromosome
  • strand: +
  • coordinates: 352968..353813
  • length: 846
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    GTGAGTGAATTGTCAAAAAGTGAAGATCAACGTATTACTAAAACAAAAGATGAACAAATT
    AAGCAAATAGATATATCGGATATCAAACCGAATCCGTATCAGCCCCGAAAAACTTTCGAT
    GAAAATCATTTAAATGATTTGGCAGATTCAATTAAGCAATATGGTATTTTGCAACCAATT
    GTGCTTAGAAAAACAGTTCAAGGTTATTACATTGTAGTTGGTGAAAGAAGGTTTAGAGCT
    TCGAAAATTGCTGGTCTAAAATACGTATCAGCGATTATCAAAGATTTAACAGATGAAGAT
    ATGATGGAACTGGCGGTCATCGAAAATTTACAACGAGAAGACTTAAATGCGATTGAAGAA
    GCTGAAAGTTATCAACGTTTGATGACAGATTTGAAAATTACACAACAAGAAGTAGCGAAG
    CGATTGAGTAAGTCGCGCCCGTATATAGCGAATATGTTGAGGTTATTACATTTGCCGAAA
    AAGATTGCTGACATGGTAAAAGATGGGCGACTGACAAGTGCACATGGACGAACGTTATTG
    GCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGGTTGTTAAAGAAAAGTGG
    AGTGTCAGATATTTAGAAAACCATGTTAATGAATTAAAAAATGTTTCGTCAAAGTCGGAA
    ACAGACAAAGTAGATATAACTAAGCCTAAATTTATAAAGCAGCAAGAACGACAGTTGCGA
    GAACAGTATGGTACCAAAGTAGATATATCAATAAAAAAATCGGTTGGTAAAATCTCATTT
    GAGTTTGATTCACAAGAAGATTTTCTAAGAATAATTGAACAATTAAATCGTAGGTATGGT
    AAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    846

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_000314
  • symbol: JSNZ_000314
  • description: ParB/RepB/Spo0J family partition protein
  • length: 281
  • theoretical pI: 10.0114
  • theoretical MW: 32869.6
  • GRAVY: -0.713879

⊟Function[edit | edit source]

  • TIGRFAM:
    nucleoid occlusion protein (TIGR04285; HMM-score: 248.1)
    and 3 more
    ParB/RepB/Spo0J family partition protein (TIGR00180; HMM-score: 195.1)
    PRTRC system ParB family protein (TIGR03734; HMM-score: 118.7)
    Genetic information processing Mobile and extrachromosomal element functions Plasmid functions plasmid partitioning protein RepB (TIGR03454; HMM-score: 70.8)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    ParBc (CL0248) ParBc; ParB/Sulfiredoxin domain (PF02195; HMM-score: 102.7)
    and 9 more
    HTH (CL0123) HTH_ParB; HTH domain found in ParB protein (PF17762; HMM-score: 60.7)
    no clan defined ParB_dimer; ParB dimerisation domain (PF23552; HMM-score: 21.5)
    HTH (CL0123) DUF1016_N; DUF1016 N-terminal domain (PF17761; HMM-score: 19)
    HTH_3; Helix-turn-helix (PF01381; HMM-score: 17.4)
    no clan defined Latarcin; Latarcin precursor (PF10279; HMM-score: 15)
    DUF3861; Domain of Unknown Function with PDB structure (DUF3861) (PF12977; HMM-score: 13.5)
    HTH (CL0123) MerR_2; MerR HTH family regulatory protein (PF13591; HMM-score: 13.1)
    Ubiquitin (CL0072) Ubiquitin_5; Ubiquitin-like domain (PF18037; HMM-score: 12.3)
    HTH (CL0123) HTH_Crp_2; Crp-like helix-turn-helix domain (PF13545; HMM-score: 11.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9633
    • Cytoplasmic Membrane Score: 0.0095
    • Cell wall & surface Score: 0.0021
    • Extracellular Score: 0.0252
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007656
    • TAT(Tat/SPI): 0.000789
    • LIPO(Sec/SPII): 0.000953
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MSELSKSEDQRITKTKDEQIKQIDISDIKPNPYQPRKTFDENHLNDLADSIKQYGILQPIVLRKTVQGYYIVVGERRFRASKIAGLKYVSAIIKDLTDEDMMELAVIENLQREDLNAIEEAESYQRLMTDLKITQQEVAKRLSKSRPYIANMLRLLHLPKKIADMVKDGRLTSAHGRTLLAIKDEQQMLRLAKRVVKEKWSVRYLENHVNELKNVSSKSETDKVDITKPKFIKQQERQLREQYGTKVDISIKKSVGKISFEFDSQEDFLRIIEQLNRRYGK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: LacR* (repression) regulon, CodY (repression) regulon
    LacR*(TF)important in Lactose utilization;  regulation predicted or transferred from N315 and NCTC 8325  [1]
    CodY(TF)important in Amino acid metabolism;  regulation predicted or transferred from N315 and NCTC 8325  [1]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.0 1.1 Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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