PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

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⊟Summary

• organism: Staphylococcus aureus N315
locus tag: SA0348 [new locus tag: SA_RS01990 ]
pan locus tag^?: SAUPAN001906000
symbol: SA0348
pan gene symbol^?: parB
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA0348 [new locus tag: SA_RS01990 ]
• symbol: SA0348
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 408141..408977
• length: 837
• essential: yes DEG other strains

⊟Accession numbers

• Gene ID: 1123127 NCBI
• RefSeq: NP_373594 NCBI
• BioCyc: see SA_RS01990
• MicrobesOnline: 102620 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
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  TTGTCAAAAAGTGAAGATCAACGTATTACTAAAACAAAAGATGAACAAATTAAGCAAATA
  GATATATCGGATATCAAACCGAATCCGTATCAGCCCCGAAAAACTTTCGATGAAAATCAT
  TTAAATGATTTGGCAGATTCAATTAAGCAATATGGAATTTTGCAACCAATTGTGCTTAGA
  AAAACAGTTCAAGGTTATTACATTGTAGTTGGTGAAAGAAGGTTTAGAGCTTCGAAAATT
  GCTGGTCTAAAATACGTATCAGCGATTATCAAAGATTTAACAGATGAAGATATGATGGAA
  CTGGCGGTCATCGAAAATTTACAACGAGAAGACTTAAATGCGATTGAAGAAGCTGAAAGT
  TATCAACGTTTGATGACAGATTTGAAAATTACACAACAAGAAGTAGCGAAGCGATTGAGT
  AAGTCGCGCCCGTATATAGCGAATATGTTGAGGTTATTACATTTGCCGAAAAAGATTGCT
  GACATGGTAAAAGATGGGCGACTGACAAGTGCACATGGACGAACGTTATTGGCAATTAAA
  GATGAACAACAAATGCTTAGGTTAGCGAAACGGGTTGTTAAAGAAAAGTGGAGTGTTAGA
  TATTTAGAAAACCATGTTAATGAATTAAAAAATGTTTCGTCAAAGTCGGAAACAGACAAA
  GTAGATATAACTAAGCCTAAATTTATAAAGCAACAAGAACGACAGTTGCGAGAACAGTAT
  GGTACCAAAGTAGATATATCAATAAAAAAATCGGTTGGTAAAATCTCATTTGAGTTTGAT
  TCACAAGATGACTTTGTGAGAATAATTGAACAATTAAATCGTAGGTATGGTAAATAG
  60
  120
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  837
  

⊟Protein

⊟General

• locus tag: SA0348 [new locus tag: SA_RS01990 ]
• symbol: SA0348
• description: hypothetical protein
• length: 278
• theoretical pI: 10.0767
• theoretical MW: 32512.2
• GRAVY: -0.718345

⊟Function

• ⊞TIGRFAM:
  nucleoid occlusion protein (TIGR04285; HMM-score: 248.8)
  and 3 more
  ParB/RepB/Spo0J family partition protein (TIGR00180; HMM-score: 195.1)
  PRTRC system ParB family protein (TIGR03734; HMM-score: 118.7)
  Genetic information processing Mobile and extrachromosomal element functions Plasmid functions plasmid partitioning protein RepB (TIGR03454; HMM-score: 70.5)
• ⊞TheSEED  :
  • Chromosome (plasmid) partitioning protein ParB
  Cell Division and Cell Cycle Cell Division and Cell Cycle - no subcategory Bacterial Cytoskeleton  Chromosome (plasmid) partitioning protein ParB
  and 1 more
  Cell Division and Cell Cycle Cell Division and Cell Cycle - no subcategory Two cell division clusters relating to chromosome partitioning  Chromosome (plasmid) partitioning protein ParB
• ⊞PFAM:
  ParBc (CL0248) ParBc; ParB/Sulfiredoxin domain (PF02195; HMM-score: 102.7)
  and 10 more
  HTH (CL0123) HTH_ParB; HTH domain found in ParB protein (PF17762; HMM-score: 60.7)
  no clan defined ParB_dimer; ParB dimerisation domain (PF23552; HMM-score: 24.1)
  HTH (CL0123) DUF1016_N; DUF1016 N-terminal domain (PF17761; HMM-score: 18.6)
  HTH_3; Helix-turn-helix (PF01381; HMM-score: 17.3)
  no clan defined Latarcin; Latarcin precursor (PF10279; HMM-score: 15)
  DUF3861; Domain of Unknown Function with PDB structure (DUF3861) (PF12977; HMM-score: 14.6)
  HTH (CL0123) MerR_2; MerR HTH family regulatory protein (PF13591; HMM-score: 13)
  Ubiquitin (CL0072) Ubiquitin_5; Ubiquitin-like domain (PF18037; HMM-score: 12.1)
  HTH (CL0123) HTH_Crp_2; Crp-like helix-turn-helix domain (PF13545; HMM-score: 11.9)
  no clan defined ThiI_fer; ThiI ferredoxin-like domain (PF22025; HMM-score: 9.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• ⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9618
  • Cytoplasmic Membrane Score: 0.0097
  • Cell wall & surface Score: 0.0019
  • Extracellular Score: 0.0267
• ⊞LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.010282
  • TAT(Tat/SPI): 0.000757
  • LIPO(Sec/SPII): 0.001015
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15926061 NCBI
• RefSeq: NP_373594 NCBI
• UniProt: A0A0H3JNP2 UniProt

⊟Protein sequence

• MSKSEDQRITKTKDEQIKQIDISDIKPNPYQPRKTFDENHLNDLADSIKQYGILQPIVLRKTVQGYYIVVGERRFRASKIAGLKYVSAIIKDLTDEDMMELAVIENLQREDLNAIEEAESYQRLMTDLKITQQEVAKRLSKSRPYIANMLRLLHLPKKIADMVKDGRLTSAHGRTLLAIKDEQQMLRLAKRVVKEKWSVRYLENHVNELKNVSSKSETDKVDITKPKFIKQQERQLREQYGTKVDISIKKSVGKISFEFDSQDDFVRIIEQLNRRYGK

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• ⊞regulator: CodY (repression) regulon

  CodY

  (TF)

  important in Amino acid metabolism; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications