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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_000136
  • pan locus tag?: SAUPAN001036000
  • symbol: JSNZ_000136
  • pan gene symbol?: murR
  • synonym:
  • product: MurR/RpiR family transcriptional regulator

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_000136
  • symbol: JSNZ_000136
  • product: MurR/RpiR family transcriptional regulator
  • replicon: chromosome
  • strand: +
  • coordinates: 162278..163153
  • length: 876
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGACAAATATTTTATATCGCATTGATAAGCAGTTGAGTGATTTTACGAAGACAGAAAAG
    ATAATCGCTGATTACATTTTAAAGAATCCACATAAAATCATTGATATGACTGTGAATGAT
    TTGGCAGATGTTACGAATGTTAGTACAGCATCAATTGTTAGATTTAGTCGGAAAATGACA
    CATCAAGGTTTTCAAGAGCTAAAGATTGCGATATCTCGATACTTACCCGAAGATATTGCA
    ACCAATCCACATTTAGAATTGATTGAAAATGAATCTGTAGAAACTTTGAAAAATAAAATG
    ATTGCTAGAGCAACGAATACGATGCGATTTGTAGCTACTAATATTATGGATGCGCAAATT
    GATGCAATTTGTGATGTGTTGAAAAATGCCAGGACAATATTTTTATTTGGATTTGGCGCA
    TCGAGTTTGACTATTGGTGATCTTTTTCAAAAGTTATCTCGTATTGGCTTAAATGTCAGG
    TTATTACATGAAACGCATTTACTTGTGTCAACATTTGCGACGCATGATGATAGAGATTGC
    ATGATTTTTGTGACGAATCAAGGTAGTCATAGTGAATTGCAGTCAATTGCACAGGTGGCC
    ACACATTACAGTATTCCCATCATAACTATATCTAGTACAGCTAATAATCCAGTGGCTCAA
    ATTGCAGACTATGCATTGATTTATGGCAGAACTGATGAAAATGAAATGCGTATGGCGGCT
    ACAACGTCACTATTTGCACAGTTATTCACGGTAGATATATTGTACTATCGATTTGTAGCA
    TTAAATTATCATGCGATTCTAGATTGTATAACCCAATCGAAAATGGCACTTGATAATTAC
    AGGAAGCATCTTGCGACGATAGATTTTAAACATTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    876

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_000136
  • symbol: JSNZ_000136
  • description: MurR/RpiR family transcriptional regulator
  • length: 291
  • theoretical pI: 6.91264
  • theoretical MW: 32986.7
  • GRAVY: 0.00274914

⊟Function[edit | edit source]

  • TIGRFAM:
    6-phospho 3-hexuloisomerase (TIGR03127; EC 5.3.-.-; HMM-score: 41.6)
    and 1 more
    Metabolism Energy metabolism Sugars sugar isomerase, KpsF/GutQ family (TIGR00393; HMM-score: 29.6)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    HTH (CL0123) HTH_6; Helix-turn-helix domain, rpiR family (PF01418; HMM-score: 80)
    and 8 more
    SIS (CL0067) SIS; SIS domain (PF01380; HMM-score: 55.4)
    SIS_2; SIS domain (PF13580; HMM-score: 16.7)
    no clan defined DUF2325; Uncharacterized protein conserved in bacteria (DUF2325) (PF10087; HMM-score: 14.9)
    HTH (CL0123) MarR; MarR family (PF01047; HMM-score: 14.8)
    MCRS_N; N-terminal region of micro-spherule protein (PF13325; HMM-score: 13.4)
    MarR_2; MarR family (PF12802; HMM-score: 13.2)
    HTH_Crp_2; Crp-like helix-turn-helix domain (PF13545; HMM-score: 12.2)
    Calcineurin (CL0163) PhoD_2; PhoD related phosphatase (PF19050; HMM-score: 11.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effector: N-acetylmuramate-6-phosphate
  • genes regulated by MurR*, TF important in N-acetylmuramate utilizationsee RegPrecise for N315
    repression
    mupG > murQ > murP* > murR*
    cxaR*
    sceD
    scrA

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9756
    • Cytoplasmic Membrane Score: 0.0131
    • Cell wall & surface Score: 0.0002
    • Extracellular Score: 0.011
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002902
    • TAT(Tat/SPI): 0.000097
    • LIPO(Sec/SPII): 0.000253
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MTNILYRIDKQLSDFTKTEKIIADYILKNPHKIIDMTVNDLADVTNVSTASIVRFSRKMTHQGFQELKIAISRYLPEDIATNPHLELIENESVETLKNKMIARATNTMRFVATNIMDAQIDAICDVLKNARTIFLFGFGASSLTIGDLFQKLSRIGLNVRLLHETHLLVSTFATHDDRDCMIFVTNQGSHSELQSIAQVATHYSIPIITISSTANNPVAQIADYALIYGRTDENEMRMAATTSLFAQLFTVDILYYRFVALNYHAILDCITQSKMALDNYRKHLATIDFKH

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: MurR* (repression) regulon, CcpA regulon
    MurR*(TF)important in N-acetylmuramate utilization;  regulation predicted or transferred from N315 and NCTC 8325  [2]
    CcpA(TF)important in Carbon catabolism;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ 2.0 2.1 Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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