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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_000050
  • pan locus tag?: SAUPAN000902000
  • symbol: JSNZ_000050
  • pan gene symbol?: —
  • synonym:
  • product: oleate hydratase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_000050
  • symbol: JSNZ_000050
  • product: oleate hydratase
  • replicon: chromosome
  • strand: -
  • coordinates: 58858..60633
  • length: 1776
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGTATTACAGTTATGGAAATTATGAAGCATTTGCGCGCCCTAAAAAACCTGAAAATGTA
    GAAAACAAATCTGCTTACTTAATCGGGTCTGGGTTAGCTTCACTTGCGGCAGCTTGTTTC
    TTAATTAGGGATGGTCAAATGGAAGGTTCGAAGATTCATATTTTAGAAGAGTTACCTAAA
    GCAGGTGGTAGTCTTGACGGAGAAAATATTCCTTTAAAAGGCTATGTTGTCCGCGGTGGT
    CGTGAAATGGAGAACCACTTTGAATGTTTGTGGGACTTATTCAGATCTATCCCTTCATTA
    GAAATCGATAACGCGTCAGTATTAGATGAATTCTATTGGTTAAACAAAGAAGACCCTAAC
    TATTCTCGCTGTCGTGTTATTGAAAAACAGGGTCAACGTTTAGTTACCGATGGAGACTTC
    ACTTTGACCAAAAAAGCGATTAAAGAAATTTTAGATTTATGCTTAACGAATGAAGAAGAT
    TTAGATGATGTCAAAATAACAGATGTATTTTCCGATGACTTCTTTAATTCAAACTTTTGG
    ATTTACTGGAAAACGATGTTTGCATTTGAACCGTGGCATTCTGCTATGGAAATGCGTCGC
    TATCTAATGCGATTCGTTCATCATATTGGTGGTTTCGCAGACTTTTCAGCTTTAAAATTC
    ACTAAATATAATCAATATGAATCTTTAGTATTACCTATGGTTGAATATTTAAAATCGCAT
    GGGGTTCAATTTGAATACGATGTAAAAGTAGAAGATATTAAAGTAGATGTTACGACAAGT
    CAAAAAATTGCCCGAGAAATATTAATTCACCGACATGGCAAAGCAGAGTCAATTAAACTG
    ACTGTAGATGATCTTGTCTTTGTGACAAACGGTAGTATTACGGAAAGCTCTACGTATGGT
    GATAATGATACACCAGCACCACCAACTGACGAATTAGGTGGTAGTTGGACACTATGGAAA
    AATTTAGCGCGACAAAGTCCTGAATTTGGTAATCCTGATAAGTTTTGCCAAAATATTCCT
    CAAAAGAGTTGGTTTGTTTCTGCAACATCGACAACAAACAATAAAGATATTATCGATACA
    ATAGAAAGCATTTGTAAACGTGACCCTCTTGCAGGCAAAACGGTTACTGGCGGAATTATT
    ACAATCAATGATTCTGCATGGCAAATCAGTTTTACAATCAATCGTCAGCAACAGTTTAAA
    GACCAACCTAAAAATGAAATATCTACGTGGATTTATGCTTTATATTCAGATGTAAACGGC
    GATTATATTAAAAAGCCAATTACAGAATGTAGTGGTAATGAAATATGTCAAGAATGGCTA
    TATCACTTAGGTGTACCAACTGACAAAATTGAAGACTTAGCAAAACATGCATCTAATACG
    ATTCCTGTTTATATGCCATATATTACCTCTTATTTCATGACGCGTGCTATCGGCGACAGA
    CCTTTAGTCGTTCCGCATCAATCACAAAATTTAGCATTTATTGGTAACTTTGCAGAAACA
    GAGCGAGACACTGTATTTACTACAGAATATTCGGTTCGTACTGCCATGGAAGCTGTTTAT
    CAATTACTAAATATAGATCGTGGTATTCCAGAGGTCATCAATAGTACATTTGATCTTCGC
    GTCTTAATGGATGCTGTATATGAACTGAATGACCATCAAGATTTGCGCGAGATTACTAAA
    GATTCGAAAATGCAAAAACTCGCATTAGCAGGATTCCTTAAAAAGATAAAAGGTACGTAC
    ATTGAGTCATTATTAAAAGAACACAAATTGTTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1776

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_000050
  • symbol: JSNZ_000050
  • description: oleate hydratase
  • length: 591
  • theoretical pI: 4.97388
  • theoretical MW: 67660.2
  • GRAVY: -0.369882

⊟Function[edit | edit source]

  • reaction:
    EC 4.2.1.53?  ExPASy
    Oleate hydratase (R)-10-hydroxystearate = oleate + H2O
  • TIGRFAM:
    9,9'-di-cis-zeta-carotene desaturase (TIGR02732; EC 1.3.5.6; HMM-score: 15.6)
    squalene-associated FAD-dependent desaturase (TIGR03467; HMM-score: 14.6)
    and 1 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other phytoene desaturase (TIGR02734; EC 1.14.99.-; HMM-score: 10.2)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    NADP_Rossmann (CL0063) MCRA; MCRA family (PF06100; HMM-score: 750.4)
    and 2 more
    NAD_binding_8; NAD(P)-binding Rossmann-like domain (PF13450; HMM-score: 23.1)
    AB_hydrolase (CL0028) DUF6792; Domain of unknown function (DUF6792) (PF20591; HMM-score: 15.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helix: 1
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.4019
    • Cytoplasmic Membrane Score: 0.4079
    • Cell wall & surface Score: 0.0007
    • Extracellular Score: 0.1894
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.031951
    • TAT(Tat/SPI): 0.005459
    • LIPO(Sec/SPII): 0.191818
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MYYSYGNYEAFARPKKPENVENKSAYLIGSGLASLAAACFLIRDGQMEGSKIHILEELPKAGGSLDGENIPLKGYVVRGGREMENHFECLWDLFRSIPSLEIDNASVLDEFYWLNKEDPNYSRCRVIEKQGQRLVTDGDFTLTKKAIKEILDLCLTNEEDLDDVKITDVFSDDFFNSNFWIYWKTMFAFEPWHSAMEMRRYLMRFVHHIGGFADFSALKFTKYNQYESLVLPMVEYLKSHGVQFEYDVKVEDIKVDVTTSQKIAREILIHRHGKAESIKLTVDDLVFVTNGSITESSTYGDNDTPAPPTDELGGSWTLWKNLARQSPEFGNPDKFCQNIPQKSWFVSATSTTNNKDIIDTIESICKRDPLAGKTVTGGIITINDSAWQISFTINRQQQFKDQPKNEISTWIYALYSDVNGDYIKKPITECSGNEICQEWLYHLGVPTDKIEDLAKHASNTIPVYMPYITSYFMTRAIGDRPLVVPHQSQNLAFIGNFAETERDTVFTTEYSVRTAMEAVYQLLNIDRGIPEVINSTFDLRVLMDAVYELNDHQDLREITKDSKMQKLALAGFLKKIKGTYIESLLKEHKLL

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SigB (activation) regulon
    SigB(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation; regulation predicted or transferred from N315 and NCTC 8325 in Wolfgramm et al. (https://doi.org/10.1101/2025.09.03.674026)   other strains

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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