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NCBI: 01-DEC-2025

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: EGJ38_001355 [new locus tag: EGJ38_RS06765 ]
  • pan locus tag?: SAUPAN003766000
  • symbol: trpF
  • pan gene symbol?: trpF
  • synonym:
  • alternate name: JSNZ_001355
  • product: phosphoribosylanthranilate isomerase

⊟Additional information (user-provided)[edit | edit source]

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: EGJ38_001355 [new locus tag: EGJ38_RS06765 ]
  • symbol: trpF
  • product: phosphoribosylanthranilate isomerase
  • replicon: chromosome
  • strand: +
  • coordinates: 1370001..1370633
  • length: 633
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq: MGT2423319 NCBI
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

⊟Additional information (user-provided)[edit | edit source]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGAAATTGAAATTTTGTGGCTTTACATCAATTAAGGATGTTACAGCGGCCAGTCAATTA
    CCTATTGATGCGATAGGTTTCATCCATTATGAAAAAAGTAAAAGGCATCAAACAATTACC
    CAAATAAAAAAGTTAGCGTCTGCTGTTCCAAATCATATCGATAAAGTATGTGTCATGGTA
    AATCCTGATTTAACAACAATTGAACACGTATTAAGCAATACGTCAATTAACACAATACAG
    TTACACGGCACAGAATCTATTGATTTTATACAGGAAATTAAAAAGAAATATTCAAGCATT
    AAAATCACTAAAGCTTTAGCTGCAGATGAAAACATAATCCAAAACATAAATAAATATAAA
    GGGTTCGTAGATTTATTTATTATCGACACACCCTCAGTGTCGTATGGTGGTACCGGTCAA
    ACATATGACTGGACTATTTTGAAGCACATAAAAGACATACCTTACTTGATAGCAGGAGGC
    ATTAACTCTGAAAATATTCAAACAGTTAATCAACTTAAATTATCACATCAAGGTTTTGAT
    CTTGCATCAGGTATAGAAGTAAATGGGCGAAAAGATATAGAAAAAATGACAGCAATTGTA
    AATATTGTGAAAGGAGATAGAGAAAATGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    633


⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: EGJ38_001355 [new locus tag: EGJ38_RS06765 ]
  • symbol: TrpF
  • description: phosphoribosylanthranilate isomerase
  • length: 210
  • theoretical pI: 8.2277
  • theoretical MW: 23386.7
  • GRAVY: -0.181905

⊟Function[edit | edit source]

  • reaction:
    EC 5.3.1.24?  ExPASy
    Phosphoribosylanthranilate isomerase N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
  • TIGRFAM:
    His-Xaa-Ser system putative quinone modification maturase (TIGR03981; HMM-score: 12.1)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    TIM_barrel (CL0036) PRAI; N-(5'phosphoribosyl)anthranilate (PRA) isomerase (PF00697; HMM-score: 129.1)
    and 2 more
    PIN (CL0280) 5_3_exonuc_N; 5'-3' exonuclease, N-terminal resolvase-like domain (PF02739; HMM-score: 13.8)
    no clan defined DUF1995; Domain of unknown function (DUF1995) (PF09353; HMM-score: 13.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9964
    • Cytoplasmic Membrane Score: 0.0017
    • Cell wall & surface Score: 0.0003
    • Extracellular Score: 0.0017
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.011139
    • TAT(Tat/SPI): 0.000449
    • LIPO(Sec/SPII): 0.001166
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: MGT2423319 NCBI
  • UniProt:

⊟Additional information (user-provided)[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKLKFCGFTSIKDVTAASQLPIDAIGFIHYEKSKRHQTITQIKKLASAVPNHIDKVCVMVNPDLTTIEHVLSNTSINTIQLHGTESIDFIQEIKKKYSSIKITKALAADENIIQNINKYKGFVDLFIIDTPSVSYGGTGQTYDWTILKHIKDIPYLIAGGINSENIQTVNQLKLSHQGFDLASGIEVNGRKDIEKMTAIVNIVKGDRENE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CodY (repression) regulon
    CodY(TF)important in Amino acid metabolism;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Additional information (user-provided)[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Additional information (user-provided)[edit | edit source]

⊟Other information (user-provided)[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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