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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

organism: Staphylococcus aureus N315

locus tag: SA_RS01030 [old locus tag: SA0171 ]

pan locus tag^?: SAUPAN001016000

symbol: SA_RS01030

pan gene symbol^?: fdh

synonym:
product: formate dehydrogenase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

type: CDS
locus tag: SA_RS01030 [old locus tag: SA0171 ]
symbol: SA_RS01030
product: formate dehydrogenase
replicon: chromosome
strand: +
coordinates: 194086..195111
length: 1026
essential: no DEG other strains

⊟Accession numbers[edit | edit source]

Location: NC_002745 (194086..195111) NCBI
BioCyc: SA_RS01030 BioCyc
MicrobesOnline: see SA0171

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGAAAATCGTAGCATTATTTCCAGAAGCAGTAGAAGGTCAAGAAAATCAATTACTTAAT
ACTAAAAAAGCAATAGGATTAAAAACATTTTTAGAGGAAAGAGGACATGAGTTCATTATA
TTAGCAGATAATGGTGAAGACTTAGATAAACATTTACCAGATATGGATGTGATTATTAGT
GCGCCATTTTATCCTGCATATATGACTCGTGAACGTATTGAAAAAGCACCGAACTTGAAA
TTAGCAATTACAGCAGGTGTAGGATCTGACCATGTAGATTTAGCGGCAGCAAGTGAACAC
AATATTGGTGTCGTTGAAGTTACAGGAAGTAATACAGTTAGTGTGGCAGAACATGCGGTT
ATGGATTTATTAATACTTCTTAGAAACTATGAAGAAGGTCATCGTCAATCAGTAGAAGGT
GAATGGAACTTGTCTCAAGTAGGTAATCATGCGCATGAATTACAACACAAAACAATTGGT
ATTTTTGGATTTGGTCGAATTGGACAACTTGTTGCTGAAAGATTAGCGCCATTTAATGTA
ACATTACAACACTATGATCCAATCAATCAACAAGACCATAAATTGTCTAAATTTGTAAGC
TTTGATGAACTTGTTTCATCAAGTGATGCGATTACAATTCATGCACCATTAACACCAGAA
ACTGATAACTTATTTGATAAAGATGTTTTAAGTCGTATGAAAAAACACAGTTATTTAGTG
AATACTGCACGTGGTAAAATTGTAAATCGCGATGCGTTAGTTGAAGCGTTAGCATCCGAG
CATTTACAAGGATATGCTGGTGATGTTTGGTATCCACAACCTGCACCTGCTGATCATCCA
TGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCA
CAAAAACGCATTGAAGATGGAGTTAAAGATATTTTAGAGCGTTTCTTCAATCATGAACCT
TTCCAAGATAAAGATATTATTGTTGCAAGTGGTCGTATTGCTAGTAAAAGTTATACAGCT
AAATAG
60
120
180
240
300
360
420
480
540
600
660
720
780
840
900
960
1020
1026

⊟Protein[edit | edit source]

⊟General[edit | edit source]

locus tag: SA_RS01030 [old locus tag: SA0171 ]
symbol: SA_RS01030
description: formate dehydrogenase
length: 341
theoretical pI: 5.92727
theoretical MW: 38103.8
GRAVY: -0.321114

⊟Function[edit | edit source]

⊞reaction:
EC 1.2.2.1? ExPASy
Formate dehydrogenase (cytochrome) Formate + 2 ferricytochrome b1 = CO₂ + 2 ferrocytochrome b1 + 2 H⁺
⊞TIGRFAM:
Metabolism Amino acid biosynthesis Serine family phosphoglycerate dehydrogenase (TIGR01327; EC 1.1.1.95; HMM-score: 204)
and 2 more
Metabolism Energy metabolism Glycolysis/gluconeogenesis glyceraldehyde-3-phosphate dehydrogenase, type I (TIGR01534; EC 1.2.1.-; HMM-score: 12.9)
Metabolism Energy metabolism Sugars inositol 2-dehydrogenase (TIGR04380; EC 1.1.1.18; HMM-score: 12.8)
TheSEED: see SA0171
⊞PFAM:
NADP_Rossmann (CL0063) 2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 182.9)
and 6 more
2-Hacid_dh; D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain (PF00389; HMM-score: 73)
Gp_dh_N; Glyceraldehyde 3-phosphate dehydrogenase, NAD binding domain (PF00044; HMM-score: 15.1)
Shikimate_DH; Shikimate / quinate 5-dehydrogenase (PF01488; HMM-score: 14.3)
KARI_N; Acetohydroxy acid isomeroreductase, NADPH-binding domain (PF07991; HMM-score: 13.9)
NAD_binding_2; NAD binding domain of 6-phosphogluconate dehydrogenase (PF03446; HMM-score: 12.9)
no clan defined DUF6315; Family of unknown function (DUF6315) (PF19836; HMM-score: 12)

⊟Structure, modifications & cofactors[edit | edit source]

domains:
modifications:
cofactors:
effectors:

⊟Localization[edit | edit source]

⊞
PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.97
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0.01
- Extracellular Score: 0.02
- Internal Helices: 0
⊞
DeepLocPro: Cytoplasmic
- Cytoplasmic Score: 0.9862
- Cytoplasmic Membrane Score: 0.0012
- Cell wall & surface Score: 0
- Extracellular Score: 0.0125
LocateP:
⊞
SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.00444
- TAT(Tat/SPI): 0.00018
- LIPO(Sec/SPII): 0.000463
predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

GI: 446628789 NCBI
RefSeq: WP_000706135 NCBI
UniProt: see SA0171

⊟Protein sequence[edit | edit source]

MKIVALFPEAVEGQENQLLNTKKAIGLKTFLEERGHEFIILADNGEDLDKHLPDMDVIISAPFYPAYMTRERIEKAPNLKLAITAGVGSDHVDLAAASEHNIGVVEVTGSNTVSVAEHAVMDLLILLRNYEEGHRQSVEGEWNLSQVGNHAHELQHKTIGIFGFGRIGQLVAERLAPFNVTLQHYDPINQQDHKLSKFVSFDELVSSSDAITIHAPLTPETDNLFDKDVLSRMKKHSYLVNTARGKIVNRDALVEALASEHLQGYAGDVWYPQPAPADHPWRTMPRNAMTVHYSGMTLEAQKRIEDGVKDILERFFNHEPFQDKDIIVASGRIASKSYTAK

⊟Experimental data[edit | edit source]

experimentally validated: data available for COL, NCTC8325
protein localization: data available for COL
quantitative data / protein copy number per cell: data available for COL
interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

regulator:

⊟Transcription pattern[edit | edit source]

S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

Aureolib: data available for COL

⊟Protein stability[edit | edit source]

half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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