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From AureoWiki
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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

Contents

  • 1 Summary
  • 2 Genome View
  • 3 Gene
    • 3.1 General
    • 3.2 Accession numbers
    • 3.3 Phenotype
    • 3.4 DNA sequence
  • 4 Protein
    • 4.1 General
    • 4.2 Function
    • 4.3 Structure, modifications & cofactors
    • 4.4 Localization
    • 4.5 Accession numbers
    • 4.6 Protein sequence
    • 4.7 Experimental data
  • 5 Expression & Regulation
    • 5.1 Operon
    • 5.2 Regulation
    • 5.3 Transcription pattern
    • 5.4 Protein synthesis (provided by Aureolib)
    • 5.5 Protein stability
  • 6 Biological Material
    • 6.1 Mutants
    • 6.2 Expression vector
    • 6.3 lacZ fusion
    • 6.4 GFP fusion
    • 6.5 two-hybrid system
    • 6.6 FLAG-tag construct
    • 6.7 Antibody
  • 7 Other Information
  • 8 Literature
    • 8.1 References
    • 8.2 Relevant publications
  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00972
  • pan locus tag?: SAUPAN003232000
  • symbol: SAOUHSC_00972
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_00972
  • symbol: SAOUHSC_00972
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 946912..947199
  • length: 288
  • essential: no [1] DEG

⊟Accession numbers[edit | edit source]

  • Gene ID: 3920117 NCBI
  • RefSeq: YP_499525 NCBI
  • BioCyc: G1I0R-916 BioCyc
  • MicrobesOnline: 1289438 MicrobesOnline

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    ATGGAAAAGGGGATTTTCAATTACGATAATGCGAATGTATTAAAATTAGATACTAATCAG
    TTAAATGAAAACATAAAAGTCATTGACGATATATTTAAAAATTATGAGCAGATAGAGCCA
    ACTATAGAAGTCGAAAATGGAAATACAAAATTAAAATTAAATGGATATTTTATTGCGTCA
    ATTATAAGCCCATTAAATTTAAATAAACTTAATAATTTATATGTTGAAGAAGAATTTTAT
    CATACATATAACGAATTAATTGTTAAATATACTGAGGTAAAGGAATAA
    60
    120
    180
    240
    288

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_00972
  • symbol: SAOUHSC_00972
  • description: hypothetical protein
  • length: 95
  • theoretical pI: 4.29329
  • theoretical MW: 11193.5
  • GRAVY: -0.5

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • FIG01108706: hypothetical protein
  • PFAM:
    Concanavalin (CL0004) Glu2-Pro; Glu2-Pro domain (PF22875; HMM-score: 15.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • ⊞⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.5869
    • Cytoplasmic Membrane Score: 0.0039
    • Cell wall & surface Score: 0.0003
    • Extracellular Score: 0.4089
  • ⊞⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006034
    • TAT(Tat/SPI): 0.000157
    • LIPO(Sec/SPII): 0.000508
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI: 88194729 NCBI
  • RefSeq: YP_499525 NCBI
  • UniProt: Q2FZM8 UniProt
  • STRING: 93061.SAOUHSC_00972 STRING

⊟Protein sequence[edit | edit source]

  • MEKGIFNYDNANVLKLDTNQLNENIKVIDDIFKNYEQIEPTIEVENGNTKLKLNGYFIASIISPLNLNKLNNLYVEEEFYHTYNELIVKYTEVKE

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [2] [3]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

  • predicted SigA promoter [4] : SAOUHSC_00972 > SAOUHSC_00973

⊟Regulation[edit | edit source]

  • ⊞⊞regulator: GraR* (activation) regulon
    GraR*(response regulator)important in cationic antimicrobial peptide (CAMP) resistance;  [5]

⊟Transcription pattern[edit | edit source]

  • S.aureus Expression Data Browser:  [4] 
    Expression Data Browser
    ⊟⊟Multi-gene expression profiles



    Click on any data point to display a description of the corresponding condition!

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

  • Aureolib: no data available

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  4. ↑ Jump up to: 4.0 4.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)
  5. ↑ Mélanie Falord, Ulrike Mäder, Aurélia Hiron, Michel Débarbouillé, Tarek Msadek
    Investigation of the Staphylococcus aureus GraSR regulon reveals novel links to virulence, stress response and cell wall signal transduction pathways.
    PLoS One: 2011, 6(7);e21323
    [PubMed:21765893] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SAOUHSC_00972&oldid=61656"
Insert paragraph

NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00972
  • pan locus tag?: SAUPAN003232000
  • symbol: SAOUHSC_00972
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

Insert paragraph

⊟Genome View[edit | edit source]

Insert paragraph

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_00972
  • symbol: SAOUHSC_00972
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 946912..947199
  • length: 288
  • essential: no [1] DEG

  1. ↑ Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
Insert paragraph

⊟Accession numbers[edit | edit source]

  • Gene ID: 3920117 NCBI
  • RefSeq: YP_499525 NCBI
  • BioCyc: G1I0R-916 BioCyc
  • MicrobesOnline: 1289438 MicrobesOnline

Insert paragraph

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    ATGGAAAAGGGGATTTTCAATTACGATAATGCGAATGTATTAAAATTAGATACTAATCAG
    TTAAATGAAAACATAAAAGTCATTGACGATATATTTAAAAATTATGAGCAGATAGAGCCA
    ACTATAGAAGTCGAAAATGGAAATACAAAATTAAAATTAAATGGATATTTTATTGCGTCA
    ATTATAAGCCCATTAAATTTAAATAAACTTAATAATTTATATGTTGAAGAAGAATTTTAT
    CATACATATAACGAATTAATTGTTAAATATACTGAGGTAAAGGAATAA
    60
    120
    180
    240
    288

Insert paragraph

This data comes from external databases and cannot be edited.

Insert paragraph

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_00972
  • symbol: SAOUHSC_00972
  • description: hypothetical protein
  • length: 95
  • theoretical pI: 4.29329
  • theoretical MW: 11193.5
  • GRAVY: -0.5

Insert paragraph

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • FIG01108706: hypothetical protein
  • PFAM:
    Concanavalin (CL0004) Glu2-Pro; Glu2-Pro domain (PF22875; HMM-score: 15.8)

Insert paragraph

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Insert paragraph

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.5869
    • Cytoplasmic Membrane Score: 0.0039
    • Cell wall & surface Score: 0.0003
    • Extracellular Score: 0.4089
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006034
    • TAT(Tat/SPI): 0.000157
    • LIPO(Sec/SPII): 0.000508
  • predicted transmembrane helices (TMHMM): 0

Insert paragraph

⊟Accession numbers[edit | edit source]

  • GI: 88194729 NCBI
  • RefSeq: YP_499525 NCBI
  • UniProt: Q2FZM8 UniProt
  • STRING: 93061.SAOUHSC_00972 STRING

Insert paragraph

⊟Protein sequence[edit | edit source]

  • MEKGIFNYDNANVLKLDTNQLNENIKVIDDIFKNYEQIEPTIEVENGNTKLKLNGYFIASIISPLNLNKLNNLYVEEEFYHTYNELIVKYTEVKE

Insert paragraph

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
Insert paragraph

⊟Expression & Regulation[edit | edit source]

Insert paragraph

⊟Operon[edit | edit source]

  • predicted SigA promoter [1] : SAOUHSC_00972 > SAOUHSC_00973

  1. ↑ Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)
Insert paragraph

⊟Regulation[edit | edit source]

  • ⊞regulator: GraR* (activation) regulon
    GraR*(response regulator)important in cationic antimicrobial peptide (CAMP) resistance;  [1]

  1. ↑ Mélanie Falord, Ulrike Mäder, Aurélia Hiron, Michel Débarbouillé, Tarek Msadek
    Investigation of the Staphylococcus aureus GraSR regulon reveals novel links to virulence, stress response and cell wall signal transduction pathways.
    PLoS One: 2011, 6(7);e21323
    [PubMed:21765893] [WorldCat.org] [DOI] (I p)
Insert paragraph

⊟Transcription pattern[edit | edit source]

  • S.aureus Expression Data Browser:  [1] 
    Expression Data Browser
    ⊟Multi-gene expression profiles

  1. ↑ Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)
Insert paragraph

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

  • Aureolib: no data available

Insert paragraph

⊟Protein stability[edit | edit source]

  • half-life: no data available

Insert paragraph

⊞Biological Material[edit | edit source]

Insert paragraph

⊟Mutants[edit | edit source]

Insert paragraph

⊟Expression vector[edit | edit source]

Insert paragraph

⊟lacZ fusion[edit | edit source]

Insert paragraph

⊟GFP fusion[edit | edit source]

Insert paragraph

⊟two-hybrid system[edit | edit source]

Insert paragraph

⊟FLAG-tag construct[edit | edit source]

Insert paragraph

⊟Antibody[edit | edit source]

Insert paragraph

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

Insert paragraph

⊟Relevant publications[edit | edit source]

Insert paragraph
  • This page was last edited on 10 March 2016, at 16:01.
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