⊟Summary[edit | edit source]

PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
locus tag: SACOL_RS07290 [old locus tag: SACOL1431 ]
pan locus tag^?: SAUPAN003809000
symbol: SACOL_RS07290
pan gene symbol^?: dapB
• synonym:
• product: 4-hydroxy-tetrahydrodipicolinate reductase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS07290 [old locus tag: SACOL1431 ]
• symbol: SACOL_RS07290
• product: 4-hydroxy-tetrahydrodipicolinate reductase
• replicon: chromosome
• strand: +
• coordinates: 1443745..1444467
• length: 723
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (1443745..1444467) NCBI
• BioCyc: SACOL_RS07290 BioCyc
• MicrobesOnline: see SACOL1431

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  
  GTGAAAATATTACTAATTGGCTATGGCGCAATGAATCAACGCGTTGCTAGATTAGCAGAA
  GAAAAAGGACATGAAATCGTTGGGGTCATTGAAAATACACCGAAAGCAACAACGCCATAT
  CAACAATATCAACATATTGCAGATGTTAAAGGTGCCGATGTTGCAATAGATTTTTCAAAT
  CCAAATCTGCTTTTCCCTTTATTAGATGAAGATTTTCATTTGCCATTAGTTGTGGCAACA
  ACTGGCGAGAAAGAAAAACTACTTAATAAGTTAGATGAATTGAGTCAAAATATGCCTGTG
  TTTTTCAGCGCGAACATGAGTTATGGCGTTCATGCATTGACTAAAATTTTAGCAGCTGCT
  GTTCCCCTACTTGATGATTTCGACATCGAATTGACTGAGGCACATCATAATAAAAAAGTA
  GATGCACCAAGTGGTACGTTAGAAAAATTGTATGATGTGATCGTATCTTTGAAAGAAAAT
  GTAACACCTGTGTATGATAGACATGAATTAAATGAAAAACGCCAGCCACAAGATATTGGT
  ATACATTCTATTCGTGGAGGTACGATTGTCGGTGAACATGAAGTTCTATTTGCTGGCACT
  GATGAAACGATTCAAATCACGCATCGTGCACAATCAAAAGATATTTTTGCGAATGGTGCA
  ATACAAGCAGCAGAACGCTTAGTTAATAAACCAAACGGCTTTTATACGTTTGACAACTTA
  TAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  723
  

⊟Protein

• 3QY9
  PDB

⊟General

• locus tag: SACOL_RS07290 [old locus tag: SACOL1431 ]
• symbol: SACOL_RS07290
• description: 4-hydroxy-tetrahydrodipicolinate reductase
• length: 240
• theoretical pI: 5.13738
• theoretical MW: 26654.1
• GRAVY: -0.2225

⊟Function

• ⊞⊞reaction:
  EC 1.17.1.8?  ExPASy
  4-hydroxy-tetrahydrodipicolinate reductase (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD(P)⁺ + H₂O = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NAD(P)H
  EC 1.3.1.26?  ExPASy
  Transferred entry: 1.17.1.8
• ⊞⊞TIGRFAM:
  Metabolism Amino acid biosynthesis Aspartate family 4-hydroxy-tetrahydrodipicolinate reductase (TIGR00036; EC 1.17.1.8; HMM-score: 165.6)
  and 2 more
  Metabolism Amino acid biosynthesis Aspartate family dihydrodipicolinate reductase (TIGR02130; EC 1.17.1.8; HMM-score: 24.2)
  Metabolism Energy metabolism Pentose phosphate pathway 6-phosphogluconate dehydrogenase (decarboxylating) (TIGR00872; EC 1.1.1.44; HMM-score: 14.8)
• TheSEED: see SACOL1431
• ⊞⊞PFAM:
  GADPH_aa-bio_dh (CL0139) DapB_C; Dihydrodipicolinate reductase, C-terminus (PF05173; HMM-score: 135.5)
  and 4 more
  NADP_Rossmann (CL0063) DapB_N; Dihydrodipicolinate reductase, N-terminus (PF01113; HMM-score: 59.7)
  TPR (CL0020) Cse1; Cse1 (PF08506; HMM-score: 13.1)
  NADP_Rossmann (CL0063) F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 13)
  TrkA_N; TrkA-N domain (PF02254; HMM-score: 12.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.67
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 0.15
  • Extracellular Score: 0.17
  • Internal Helices: 0
• ⊞⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.999
  • Cytoplasmic Membrane Score: 0.0002
  • Cell wall & surface Score: 0.0004
  • Extracellular Score: 0.0004
• LocateP:
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.018788
  • TAT(Tat/SPI): 0.000318
  • LIPO(Sec/SPII): 0.002442
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446620889 NCBI
• RefSeq: WP_000698235 NCBI
• UniProt: see SACOL1431

⊟Protein sequence

• MKILLIGYGAMNQRVARLAEEKGHEIVGVIENTPKATTPYQQYQHIADVKGADVAIDFSNPNLLFPLLDEDFHLPLVVATTGEKEKLLNKLDELSQNMPVFFSANMSYGVHALTKILAAAVPLLDDFDIELTEAHHNKKVDAPSGTLEKLYDVIVSLKENVTPVYDRHELNEKRQPQDIGIHSIRGGTIVGEHEVLFAGTDETIQITHRAQSKDIFANGAIQAAERLVNKPNGFYTFDNL

⊟Experimental data

• experimentally validated: see SACOL1431
• protein localization: see SACOL1431
• quantitative data / protein copy number per cell: see SACOL1431
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: L-box, CodY see SACOL1431

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: see SACOL1431

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications