⊟Summary[edit | edit source]

PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
locus tag: SA1059 [new locus tag: SA_RS06010 ]
pan locus tag^?: SAUPAN003499000
symbol: SA1059
pan gene symbol^?: fmt
• synonym:
• product: methionyl-tRNA formyltransferase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA1059 [new locus tag: SA_RS06010 ]
• symbol: SA1059
• product: methionyl-tRNA formyltransferase
• replicon: chromosome
• strand: +
• coordinates: 1198301..1199236
• length: 936
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1123890 NCBI
• RefSeq: NP_374332 NCBI
• BioCyc: see SA_RS06010
• MicrobesOnline: 103358 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGACTAAAATAATATTTATGGGTACACCAGACTTTTCAACAACTGTTTTAGAAATGCTT
  ATTGCAGAACATGATGTCATTGCAGTCGTAACGCAACCAGATCGACCTGTTGGACGTAAA
  CGTGTTATGACACCACCACCAGTTAAAAAAGTTGCAATGAAATATGATTTACCTGTATAT
  CAGCCTGAAAAATTAAGTGGATCAGAAGAATTAGAACAATTGCTTCAATTAGATGTAGAT
  TTAATTGTAACTGCTGCTTTTGGACAATTATTACCTGAATCATTGTTGGCATTACCAAAA
  CTTGGCGCAATTAATGTACATGCATCATTGTTACCAAAGTATAGAGGTGGTGCACCAATT
  CATCAGGCAATTATCGATGGTGAACAAGAAACCGGCATAACAATTATGTATATGGTTAAA
  AAATTAGATGCGGGTAATATTATTTCGCAACAAGCAATTAAAATAGAAGAAAATGATAAT
  GTCGGTACGATGCATGATAAATTAAGTGTATTAGGGGCAGATTTATTAAAAGAAACTTTA
  CCATCTATTATAGAGGGCACAAATGAAAGTGTACCTCAAGATGATACGCAAGCAACATTT
  GCTTCCAATATTCGACGCGAAGATGAGCGAATTAGCTGGAATAAACCAGGAAGACAAGTG
  TTTAATCAAATTCGTGGATTATCACCATGGCCAGTTGCTTATACAACTATGGATGACACT
  AACTTGAAAATATACGATGCTGAACTCGTTGAGACTAATAAGATAAACGAGCCTGGAACC
  ATTATAGAAACGACTAAAAAAGCCATTATTGTTGCTACAAATGATAATGAAGCTGTTGCA
  ATTAAAGATATGCAATTAGCTGGGAAAAAGAGAATGTTAGCTGCCAATTATTTAAGTGGT
  GCGCAAAACACACTAGTAGGGAAGAAACTTATATGA
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  936
  

⊟Protein

⊟General

• locus tag: SA1059 [new locus tag: SA_RS06010 ]
• symbol: SA1059
• description: methionyl-tRNA formyltransferase
• length: 311
• theoretical pI: 5.08754
• theoretical MW: 34225.3
• GRAVY: -0.161415

⊟Function

• ⊞⊞reaction:
  EC 2.1.2.9?  ExPASy
  Methionyl-tRNA formyltransferase 10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) = tetrahydrofolate + N-formylmethionyl-tRNA(fMet)
• ⊞⊞TIGRFAM:
  Genetic information processing Protein synthesis tRNA aminoacylation methionyl-tRNA formyltransferase (TIGR00460; EC 2.1.2.9; HMM-score: 356.6)
  and 2 more
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylglycinamide formyltransferase (TIGR00639; EC 2.1.2.2; HMM-score: 68.4)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis formyltetrahydrofolate deformylase (TIGR00655; EC 3.5.1.10; HMM-score: 57.3)
• ⊞⊞TheSEED  :
  • Methionyl-tRNA formyltransferase (EC 2.1.2.9)
  Cofactors, Vitamins, Prosthetic Groups, Pigments Folate and pterines Folate Biosynthesis  Methionyl-tRNA formyltransferase (EC 2.1.2.9)
  and 1 more
  Protein Metabolism Protein biosynthesis Translation initiation factors bacterial  Methionyl-tRNA formyltransferase (EC 2.1.2.9)
• ⊞⊞PFAM:
  no clan defined Formyl_trans_N; Formyl transferase (PF00551; HMM-score: 141.2)
  and 2 more
  Formyl_trans_C; Formyl transferase, C-terminal domain (PF02911; HMM-score: 97.3)
  DUF7425; Domain of unknown function (DUF7425) (PF24200; HMM-score: 15.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• ⊞⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9992
  • Cytoplasmic Membrane Score: 0.0001
  • Cell wall & surface Score: 0.0001
  • Extracellular Score: 0.0006
• ⊞⊞LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.011881
  • TAT(Tat/SPI): 0.000818
  • LIPO(Sec/SPII): 0.001435
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15926799 NCBI
• RefSeq: NP_374332 NCBI
• UniProt: P99127 UniProt

⊟Protein sequence

• MTKIIFMGTPDFSTTVLEMLIAEHDVIAVVTQPDRPVGRKRVMTPPPVKKVAMKYDLPVYQPEKLSGSEELEQLLQLDVDLIVTAAFGQLLPESLLALPKLGAINVHASLLPKYRGGAPIHQAIIDGEQETGITIMYMVKKLDAGNIISQQAIKIEENDNVGTMHDKLSVLGADLLKETLPSIIEGTNESVPQDDTQATFASNIRREDERISWNKPGRQVFNQIRGLSPWPVAYTTMDDTNLKIYDAELVETNKINEPGTIIETTKKAIIVATNDNEAVAIKDMQLAGKKRMLAANYLSGAQNTLVGKKLI

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SA1058 > SA1059 > SA1060 > SA1061 > SA1062 > SA1063

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Alexander Scherl, Patrice François, Manuela Bento, Jacques M Deshusses, Yvan Charbonnier, Véronique Converset, Antoine Huyghe, Nadia Walter, Christine Hoogland, Ron D Appel, Jean-Charles Sanchez, Catherine G Zimmermann-Ivol, Garry L Corthals, Denis F Hochstrasser, Jacques Schrenzel
Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth.
J Microbiol Methods: 2005, 60(2);247-57
[PubMed:15590099] [WorldCat.org] [DOI] (P p)