⊟Summary[edit | edit source]

PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
locus tag: SACOL_RS06365 [old locus tag: SACOL1245 ]
pan locus tag^?: SAUPAN003515000
symbol: SACOL_RS06365
pan gene symbol^?: fabG
• synonym:
• product: beta-ketoacyl-ACP reductase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS06365 [old locus tag: SACOL1245 ]
• symbol: SACOL_RS06365
• product: beta-ketoacyl-ACP reductase
• replicon: chromosome
• strand: +
• coordinates: 1254334..1255068
• length: 735
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (1254334..1255068) NCBI
• BioCyc: SACOL_RS06365 BioCyc
• MicrobesOnline: see SACOL1245

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  721
  
  ATGACTAAGAGTGCTTTAGTAACAGGTGCATCAAGAGGAATTGGACGTAGTATTGCGTTA
  CAATTAGCAGAAGAAGGATATAATGTAGCAGTAAACTATGCAGGCAGCAAAGAGAAAGCT
  GAAGCAGTAGTCGAAGAAATCAAAGCTAAAGGTGTTGACAGTTTTGCGATTCAAGCAAAT
  GTTGCCGATGCTGATGAAGTTAAAGCAATGATTAAAGAAGTAGTTAGCCAATTTGGTTCT
  TTAGATGTTTTAGTAAATAATGCAGGTATTACTCGCGATAATTTATTAATGCGTATGAAA
  GAACAAGAGTGGGATGATGTTATTGACACAAACTTAAAAGGTGTATTTAACTGTATCCAA
  AAAGCAACACCACAAATGTTAAGACAACGTAGTGGTGCTATCATCAATTTATCAAGTGTT
  GTTGGAGCAGTAGGTAATCCGGGACAAGCAAACTATGTTGCAACAAAAGCAGGTGTTATT
  GGTTTAACTAAATCTGCGGCGCGTGAATTAGCATCTCGTGGTATCACTGTAAATGCAGTT
  GCACCTGGTTTTATTGTTTCTGATATGACAGATGCTTTAAGTGATGAGCTTAAAGAACAA
  ATGTTGACTCAAATTCCGTTAGCACGTTTTGGTCAAGACACAGATATTGCTAATACAGTA
  GCGTTCTTAGCATCAGACAAAGCAAAATATATTACAGGTCAAACAATCCATGTAAATGGT
  GGAATGTACATGTAA
  60
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  735
  

⊟Protein

• 3SJ7
  PDB

⊟General

• locus tag: SACOL_RS06365 [old locus tag: SACOL1245 ]
• symbol: SACOL_RS06365
• description: beta-ketoacyl-ACP reductase
• length: 244
• theoretical pI: 5.10093
• theoretical MW: 25886.4
• GRAVY: 0.0172131

⊟Function

• ⊞⊞reaction:
  EC 1.1.1.100?  ExPASy
  3-oxoacyl-[acyl-carrier-protein] reductase (3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP⁺ = 3-oxoacyl-[acyl-carrier-protein] + NADPH
• ⊞⊞TIGRFAM:
  Metabolism Fatty acid and phospholipid metabolism Biosynthesis 3-oxoacyl-[acyl-carrier-protein] reductase (TIGR01830; EC 1.1.1.100; HMM-score: 362.9)
  and 19 more
  acetoacetyl-CoA reductase (TIGR01829; EC 1.1.1.36; HMM-score: 284.5)
  Metabolism Fatty acid and phospholipid metabolism Biosynthesis putative 3-oxoacyl-(acyl-carrier-protein) reductase (TIGR01831; HMM-score: 242.1)
  3-hydroxybutyrate dehydrogenase (TIGR01963; HMM-score: 207)
  Metabolism Energy metabolism Fermentation acetoin reductases (TIGR02415; EC 1.1.1.-; HMM-score: 206.7)
  Unknown function Enzymes of unknown specificity SDR family mycofactocin-dependent oxidoreductase (TIGR03971; EC 1.1.99.-; HMM-score: 204.9)
  Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides 2-deoxy-D-gluconate 3-dehydrogenase (TIGR01832; EC 1.1.1.125; HMM-score: 197.2)
  2-hydroxycyclohexanecarboxyl-CoA dehydrogenase (TIGR03206; EC 1.1.1.-; HMM-score: 167.2)
  2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase (TIGR04316; EC 1.3.1.28; HMM-score: 162.7)
  Unknown function Enzymes of unknown specificity SDR family mycofactocin-dependent oxidoreductase (TIGR04504; EC 1.1.99.-; HMM-score: 140.8)
  pteridine reductase (TIGR02685; EC 1.5.1.33; HMM-score: 131.8)
  rhamnulose-1-phosphate aldolase/alcohol dehydrogenase (TIGR02632; EC 1.1.1.1,4.1.2.19; HMM-score: 117.4)
  cis-2,3-dihydrobiphenyl-2,3-diol dehydrogenase (TIGR03325; EC 1.3.1.56; HMM-score: 90.5)
  sepiapterin reductase (TIGR01500; EC 1.1.1.153; HMM-score: 35.7)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll light-dependent protochlorophyllide reductase (TIGR01289; EC 1.3.1.33; HMM-score: 23.1)
  methylmalonyl-CoA mutase C-terminal domain (TIGR00640; HMM-score: 17.8)
  Metabolism Energy metabolism Sugars UDP-glucose 4-epimerase GalE (TIGR01179; EC 5.1.3.2; HMM-score: 15)
  NAD dependent epimerase/dehydratase, LLPSF_EDH_00030 family (TIGR04180; HMM-score: 13.3)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids tricarboxylate carrier (TIGR00798; HMM-score: 11.8)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine glycine oxidase ThiO (TIGR02352; EC 1.4.3.19; HMM-score: 11.4)
• TheSEED: see SACOL1245
• ⊞⊞PFAM:
  NADP_Rossmann (CL0063) adh_short_C2; Enoyl-(Acyl carrier protein) reductase (PF13561; HMM-score: 239.5)
  adh_short; short chain dehydrogenase (PF00106; HMM-score: 222.8)
  and 12 more
  KR; KR domain (PF08659; HMM-score: 84.4)
  Epimerase; NAD dependent epimerase/dehydratase family (PF01370; HMM-score: 23.4)
  SDR; SDR-like rossmann domain (PF23441; HMM-score: 22.5)
  PDDEXK (CL0236) HFX_2341_N; HFX_2341-like, N-terminal (PF19810; HMM-score: 15.2)
  HTH (CL0123) MCM_WH_arc; Archaeal MCM, winged-helix (PF21120; HMM-score: 14)
  NADP_Rossmann (CL0063) NmrA; NmrA-like family (PF05368; HMM-score: 13.7)
  no clan defined B12-binding; B12 binding domain (PF02310; HMM-score: 13.3)
  SPOR; SPOR domain (PF05036; HMM-score: 13)
  DUF7336; Domain of unknown function (DUF7336) (PF24024; HMM-score: 12.9)
  NADP_Rossmann (CL0063) GDP_Man_Dehyd; GDP-mannose 4,6 dehydratase (PF16363; HMM-score: 12.8)
  NAD_binding_10; NAD(P)H-binding (PF13460; HMM-score: 12.3)
  CoA_binding_2; CoA binding domain (PF13380; HMM-score: 9.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• ⊞⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9008
  • Cytoplasmic Membrane Score: 0.06
  • Cell wall & surface Score: 0.0114
  • Extracellular Score: 0.0278
• LocateP:
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.019676
  • TAT(Tat/SPI): 0.002441
  • LIPO(Sec/SPII): 0.003352
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446089414 NCBI
• RefSeq: WP_000167269 NCBI
• UniProt: see SACOL1245

⊟Protein sequence

• MTKSALVTGASRGIGRSIALQLAEEGYNVAVNYAGSKEKAEAVVEEIKAKGVDSFAIQANVADADEVKAMIKEVVSQFGSLDVLVNNAGITRDNLLMRMKEQEWDDVIDTNLKGVFNCIQKATPQMLRQRSGAIINLSSVVGAVGNPGQANYVATKAGVIGLTKSAARELASRGITVNAVAPGFIVSDMTDALSDELKEQMLTQIPLARFGQDTDIANTVAFLASDKAKYITGQTIHVNGGMYM

⊟Experimental data

• experimentally validated: see SACOL1245
• protein localization: see SACOL1245
• quantitative data / protein copy number per cell: see SACOL1245
• ⊟⊟interaction partners:

  SACOL_RS03075		elongation factor G   (data from MRSA252)
  SACOL_RS03080		elongation factor Tu   (data from MRSA252)
  SACOL_RS05630		pyruvate dehydrogenase E1 component subunit alpha   (data from MRSA252)
  SACOL_RS07705		DNA-binding protein HU   (data from MRSA252)
  SACOL_RS11655		30S ribosomal protein S11   (data from MRSA252)
  SACOL_RS11695		30S ribosomal protein S5   (data from MRSA252)
  SACOL_RS11780		50S ribosomal protein L3   (data from MRSA252)

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: FapR* see SACOL1245

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: see SACOL1245

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications