⊟Summary[edit | edit source]

PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
locus tag: NWMN_RS02255 [old locus tag: NWMN_0398 ]
pan locus tag^?: SAUPAN002121000
symbol: NWMN_RS02255
pan gene symbol^?: hsdM1
• synonym:
• product: type I restriction-modification system subunit M

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS02255 [old locus tag: NWMN_0398 ]
• symbol: NWMN_RS02255
• product: type I restriction-modification system subunit M
• replicon: chromosome
• strand: +
• coordinates: 447091..448647
• length: 1557
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (447091..448647) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_0398

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGTCTATTACTGAAAAACAACGTCAGCAACAAGCTGAATTACATAAAAAATTATGGTCG
  ATTGCGAATGATTTAAGAGGGAATATGGATGCGAGTGAATTCCGTAATTACATTTTAGGC
  TTGATTTTCTATCGCTTCTTATCTGAAAAAGCGGAACAAGAATATGCAGATGCCTTGTCA
  GGTGAAGACATCACGTATCAAGAAGCATGGGCAGACGAAGAATACCGTGAAGACTTAAAA
  GCAGAATTAATTGACCAAGTCGGTTACTTCATTGAGCCAGAAGATTTATTCAGTGCGATG
  ATTCGTGAAATTGAAACGCAAGATTTCGATATCGAACACCTGGCGACGGCAATTCGTAAA
  GTTGAAACATCAACATTAGGTGAAGAAAGTGAAAATGACTTTATCGGTCTGTTCAGCGAT
  ATGGATTTGAGTTCAACGCGACTAGGTAACAATGTCAAAGAACGTACTGCTTTAATCTCT
  AAAGTCATGGTTAATCTTGACGACTTACCATTCGTTCACAGTGACATGGAAATTGATATG
  TTAGGTGATGCATATGAATTCCTAATTGGGCGCTTTGCGGCGACAGCGGGTAAAAAAGCA
  GGCGAGTTCTATACACCACAACAAGTATCTAAGATACTGGCGAAGATTGTCACAGACGGT
  AAAGATAAATTACGTCACGTGTATGACCCAACATGTGGTTCAGGTTCACTGTTGTTACGT
  GTTGGTAAAGAAACACAAGTGTATCGTTATTTCGGTCAAGAACGTAACAATACTACATAC
  AACTTAGCACGCATGAATATGTTATTACATGATGTGCGTTATGAGAACTTCGATATCCGT
  AATGATGACACATTGGAAAACCCAGCCTTTTTAGGCAATACATTTGATGCGGTTATTGCG
  AACCCACCGTATAGTGCGAAATGGACTGCAGATTCAAAGTTTGAAAATGACGAACGATTC
  AGTGGTTACGGCAAACTTGCGCCTAAGTCTAAAGCAGACTTTGCCTTTATTCAACACATG
  GTACATTACCTAGACGATGAAGGTACCATGGCCGTTGTACTCCCACATGGTGTATTATTC
  CGAGGTGCTGCAGAAGGTGTCATTCGTCGTTATTTAATTGAAGAAAAGAACTACTTAGAA
  GCTGTGATTGGTTTGCCAGCGAATATTTTCTATGGGACAAGTATTCCAACATGTATTTTA
  GTATTTAAAAAATGTCGCCAACAAGACGACAACGTACTATTTATCGATGCATCCAATGAT
  TTTGAAAAAGGAAAAAATCAAAATCATTTAAGCGATGCCCAAGTCGAACGTATTATAGAC
  ACATATAAGCGTAAGGAAACAATTGATAAATATAGCTACAGCGCGACACTACAAGAGATT
  GCCGATAACGATTACAACCTAAATATACCGAGATATGTCGATACATTCGAAGAAGAAGCA
  CCGATTGATTTAGATCAAGTCCAACAAGATTTGAAAAATATCGATAAAGAAATCGCAGAA
  ATTGAGCAAGAAATCAATGCATACCTGAAAGAACTTGGGGTGTTGAAAGATGAGTAA
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⊟Protein

⊟General

• locus tag: NWMN_RS02255 [old locus tag: NWMN_0398 ]
• symbol: NWMN_RS02255
• description: type I restriction-modification system subunit M
• length: 518
• theoretical pI: 4.36654
• theoretical MW: 59444.2
• GRAVY: -0.491506

⊟Function

• ⊞⊞TIGRFAM:
  Genetic information processing DNA metabolism Restriction/modification type I restriction-modification system, M subunit (TIGR00497; EC 2.1.1.72; HMM-score: 467.3)
  and 5 more
  Genetic information processing DNA metabolism Restriction/modification type II restriction m6 adenine DNA methyltransferase, Alw26I/Eco31I/Esp3I family (TIGR02987; EC 2.1.1.72; HMM-score: 37.2)
  Genetic information processing Protein fate Protein modification and repair protein-(glutamine-N5) methyltransferase, release factor-specific (TIGR03534; EC 2.1.1.-; HMM-score: 36.3)
  Genetic information processing Protein fate Protein modification and repair methyltransferase, HemK family (TIGR00536; HMM-score: 15.4)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-6Y C5,15-methyltransferase (decarboxylating), CbiT subunit (TIGR02469; EC 2.1.1.132; HMM-score: 14.4)
  Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification protein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific (TIGR03533; EC 2.1.1.-; HMM-score: 14.1)
• TheSEED: see NWMN_0398
• ⊞⊞PFAM:
  NADP_Rossmann (CL0063) N6_Mtase; N-6 DNA Methylase (PF02384; HMM-score: 378.5)
  and 7 more
  MmeI_HD-like (CL0884) HsdM_N; HsdM N-terminal domain (PF12161; HMM-score: 66.7)
  NADP_Rossmann (CL0063) Eco57I; Eco57I restriction-modification methylase (PF07669; HMM-score: 36.9)
  MTS; Methyltransferase small domain (PF05175; HMM-score: 25.5)
  Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 16.2)
  AviRa; RRNA methyltransferase AviRa (PF11599; HMM-score: 15.5)
  UPF0020; RMKL-like, methyltransferase domain (PF01170; HMM-score: 14.3)
  no clan defined Mu_Gam-Sipho_Gp157-TOTE; Mu Gam/Sipho_Gp157-like domain of the TOTE conflict systems (PF22709; HMM-score: 12.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• ⊞⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9913
  • Cytoplasmic Membrane Score: 0.0037
  • Cell wall & surface Score: 0.0004
  • Extracellular Score: 0.0047
• LocateP:
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003066
  • TAT(Tat/SPI): 0.000553
  • LIPO(Sec/SPII): 0.00066
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 445950773 NCBI
• RefSeq: WP_000028628 NCBI
• UniProt: see NWMN_0398

⊟Protein sequence

• MSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFYRFLSEKAEQEYADALSGEDITYQEAWADEEYREDLKAELIDQVGYFIEPEDLFSAMIREIETQDFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALISKVMVNLDDLPFVHSDMEIDMLGDAYEFLIGRFAATAGKKAGEFYTPQQVSKILAKIVTDGKDKLRHVYDPTCGSGSLLLRVGKETQVYRYFGQERNNTTYNLARMNMLLHDVRYENFDIRNDDTLENPAFLGNTFDAVIANPPYSAKWTADSKFENDERFSGYGKLAPKSKADFAFIQHMVHYLDDEGTMAVVLPHGVLFRGAAEGVIRRYLIEEKNYLEAVIGLPANIFYGTSIPTCILVFKKCRQQDDNVLFIDASNDFEKGKNQNHLSDAQVERIIDTYKRKETIDKYSYSATLQEIADNDYNLNIPRYVDTFEEEAPIDLDQVQQDLKNIDKEIAEIEQEINAYLKELGVLKDE

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications