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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0476 [new locus tag: SACOL_RS02410 ]
  • pan locus tag?: SAUPAN002121000
  • symbol: hsdM1
  • pan gene symbol?: hsdM1
  • synonym:
  • product: type I restriction-modification system, M subunit

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0476 [new locus tag: SACOL_RS02410 ]
  • symbol: hsdM1
  • product: type I restriction-modification system, M subunit
  • replicon: chromosome
  • strand: +
  • coordinates: 477049..478605
  • length: 1557
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGTCTATTACTGAAAAACAACGTCAGCAACAAGCTGAATTACATAAAAAATTATGGTCG
    ATTGCGAATGATTTAAGAGGGAATATGGATGCGAGTGAATTCCGTAATTACATTTTAGGC
    TTGATTTTCTATCGCTTCTTATCTGAAAAAGCGGAACAAGAATATGCAGATGCCTTGTCA
    GGTGAAGACATCACGTATCAAGAAGCATGGGCAGACGAAGAATACCGTGAAGACTTAAAA
    GCAGAATTAATTGACCAAGTCGGTTACTTCATTGAGCCAGAAGATTTATTCAGTGCGATG
    ATTCGTGAAATTGAAACGCAAGATTTCGATATCGAACACCTGGCGACGGCAATTCGTAAA
    GTTGAAACATCAACATTAGGTGAAGAAAGTGAAAATGACTTTATCGGTCTGTTCAGCGAT
    ATGGATTTGAGTTCAACGCGACTAGGTAACAATGTCAAAGAACGTACTGCTTTAATCTCT
    AAAGTCATGGTTAATCTTGACGACTTACCATTCGTTCACAGTGACATGGAAATTGATATG
    TTAGGTGATGCATATGAATTCCTAATTGGGCGCTTTGCGGCGACAGCGGGTAAAAAAGCA
    GGCGAGTTCTATACACCACAACAAGTATCTAAGATACTGGCGAAGATTGTCACAGACGGT
    AAAGATAAATTACGTCACGTGTATGACCCAACATGTGGTTCAGGTTCACTGTTGTTACGT
    GTTGGTAAAGAAACACAAGTGTATCGTTATTTCGGTCAAGAACGTAACAATACTACATAC
    AACTTAGCACGCATGAATATGTTATTACATGATGTGCGTTATGAGAACTTCGATATCCGT
    AATGATGACACATTGGAAAACCCAGCCTTTTTAGGCAATACATTTGATGCGGTTATTGCG
    AACCCACCGTATAGTGCGAAATGGACTGCAGATTCAAAGTTTGAAAATGACGAACGATTC
    AGTGGTTACGGCAAACTTGCGCCTAAGTCTAAAGCAGACTTTGCCTTTATTCAACACATG
    GTACATTACCTAGACGATGAAGGTACCATGGCCGTTGTACTCCCACATGGTGTATTATTC
    CGAGGTGCTGCAGAAGGTGTCATTCGTCGTTATTTAATTGAAGAAAAGAACTACTTAGAA
    GCTGTGATTGGTTTGCCAGCGAATATTTTCTATGGGACAAGTATTCCAACATGTATTTTA
    GTATTTAAAAAATGTCGCCAACAAGACGACAACGTACTATTTATCGATGCATCCAATGAT
    TTTGAAAAAGGAAAAAATCAAAATCATTTAAGCGATGCCCAAGTCGAACGTATTATAGAC
    ACATATAAGCGTAAGGAAACAATTGATAAATATAGCTACAGCGCGACACTACAAGAGATT
    GCCGATAACGATTACAACCTAAATATACCGAGATATGTCGATACATTCGAAGAAGAAGCA
    CCGATTGATTTAGATCAAGTCCAACAAGATTTGAAAAATATCGATAAAGAAATCGCAGAA
    ATTGAGCAAGAAATCAATGCATACCTGAAAGAACTTGGGGTGTTGAAAGATGAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1557

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0476 [new locus tag: SACOL_RS02410 ]
  • symbol: HsdM1
  • description: type I restriction-modification system, M subunit
  • length: 518
  • theoretical pI: 4.36654
  • theoretical MW: 59444.2
  • GRAVY: -0.491506

⊟Function[edit | edit source]

  • reaction:
    EC 2.1.1.-?  ExPASy
  • TIGRFAM:
    Genetic information processing DNA metabolism Restriction/modification type I restriction-modification system, M subunit (TIGR00497; EC 2.1.1.72; HMM-score: 467.3)
    and 5 more
    Genetic information processing DNA metabolism Restriction/modification type II restriction m6 adenine DNA methyltransferase, Alw26I/Eco31I/Esp3I family (TIGR02987; EC 2.1.1.72; HMM-score: 37.2)
    Genetic information processing Protein fate Protein modification and repair protein-(glutamine-N5) methyltransferase, release factor-specific (TIGR03534; EC 2.1.1.-; HMM-score: 36.3)
    Genetic information processing Protein fate Protein modification and repair methyltransferase, HemK family (TIGR00536; HMM-score: 15.4)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-6Y C5,15-methyltransferase (decarboxylating), CbiT subunit (TIGR02469; EC 2.1.1.132; HMM-score: 14.4)
    Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification protein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific (TIGR03533; EC 2.1.1.-; HMM-score: 14.1)
  • TheSEED  :
    • Type I restriction-modification system, DNA-methyltransferase subunit M (EC 2.1.1.72)
    DNA Metabolism DNA Metabolism - no subcategory Restriction-Modification System  Type I restriction-modification system, DNA-methyltransferase subunit M (EC 2.1.1.72)
    and 1 more
    DNA Metabolism DNA Metabolism - no subcategory Type I Restriction-Modification  Type I restriction-modification system, DNA-methyltransferase subunit M (EC 2.1.1.72)
  • PFAM:
    NADP_Rossmann (CL0063) N6_Mtase; N-6 DNA Methylase (PF02384; HMM-score: 372.3)
    and 7 more
    no clan defined HsdM_N; HsdM N-terminal domain (PF12161; HMM-score: 75.2)
    NADP_Rossmann (CL0063) MTS; Methyltransferase small domain (PF05175; HMM-score: 24.4)
    Eco57I; Eco57I restriction-modification methylase (PF07669; HMM-score: 23.6)
    Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 16)
    Methyltransf_11; Methyltransferase domain (PF08241; HMM-score: 14.6)
    UPF0020; Putative RNA methylase family UPF0020 (PF01170; HMM-score: 14.3)
    N6-adenineMlase; Probable N6-adenine methyltransferase (PF10237; HMM-score: 13.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003066
    • TAT(Tat/SPI): 0.000553
    • LIPO(Sec/SPII): 0.00066
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFYRFLSEKAEQEYADALSGEDITYQEAWADEEYREDLKAELIDQVGYFIEPEDLFSAMIREIETQDFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALISKVMVNLDDLPFVHSDMEIDMLGDAYEFLIGRFAATAGKKAGEFYTPQQVSKILAKIVTDGKDKLRHVYDPTCGSGSLLLRVGKETQVYRYFGQERNNTTYNLARMNMLLHDVRYENFDIRNDDTLENPAFLGNTFDAVIANPPYSAKWTADSKFENDERFSGYGKLAPKSKADFAFIQHMVHYLDDEGTMAVVLPHGVLFRGAAEGVIRRYLIEEKNYLEAVIGLPANIFYGTSIPTCILVFKKCRQQDDNVLFIDASNDFEKGKNQNHLSDAQVERIIDTYKRKETIDKYSYSATLQEIADNDYNLNIPRYVDTFEEEAPIDLDQVQQDLKNIDKEIAEIEQEINAYLKELGVLKDE

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2]
  • quantitative data / protein copy number per cell: 336 [3]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  3. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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