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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1041 [new locus tag: NWMN_RS05890 ]
  • pan locus tag?: SAUPAN003359000
  • symbol: isdA
  • pan gene symbol?: isdA
  • synonym:
  • product: iron-regulated heme-iron binding protein IsdA

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_1041 [new locus tag: NWMN_RS05890 ]
  • symbol: isdA
  • product: iron-regulated heme-iron binding protein IsdA
  • replicon: chromosome
  • strand: -
  • coordinates: 1144220..1145272
  • length: 1053
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGACAAAACATTATTTAAACAGTAAGTATCAATCAGAACAACGTTCATCAGCTATGAAA
    AAGATTACAATGGGTACAGCATCTATCATTTTAGGTTCCCTTGTATACATAGGCGCAGAC
    AGCCAACAAGTCAATGCGGCAACAGAAGCTACGAACGCAACTAATAATCAAAGCACACAA
    GTTTCTCAAGCAACATCACAACCAATTAATTTCCAAGTGCAAAAAGATGGCTCTTCAGAG
    AAGTCACACATGGATGACTATATGCAACACCCTGGTAAAGTAATTAAACAAAATAATAAA
    TATTATTTCCAAACCGTGTTAAACAATGCATCATTCTGGAAAGAATACAAATTTTACAAT
    GCAAACAATCAAGAATTAGCAACAACTGTTGTTAACGATAATAAAAAAGCGGATACTAGA
    ACAATCAATGTTGCAGTTGAACCTGGATATAAGAGCTTAACTACTAAAGTACATATTGTC
    GTGCCACAAATTAATTACAATCATAGATATACTACGCATTTGGAATTTGAAAAAGCAATT
    CCTACATTAGCTGACGCAGCAAAACCAAACAATGTTAAACCGGTTCAACCAAAACCAGCT
    CAACCTAAAACACCTACTGAGCAAACTAAACCAGTTCAACCTAAAGTTGAAAAAGTTAAA
    CCTACTGTAACTACAACAAGCAAAGTTGAAGACAATCACTCTACTAAAGTTGTAAGTACT
    GACACAACAAAAGATCAAACTAAAACACAAACTGCTCATACAGTTAAAACAGCACAAACT
    GCTCAAGAACAAAATAAAGTTCAAACACCTGTTAAAGATGTTGCAACAGCGAAATCTGAA
    AGCAACAATCAAGCTGTAAGTGATAATAAATCACAACAAACTAACAAAGTTACAAAACAT
    AACGAAACGCCTAAACAAGCATCTAAAGCTAAAGAATTACCAAAAACTGGTTTAACTTCA
    GTTGATAACTTTATTAGCACAGTTGCCTTCGCAACACTTGCCCTTTTAGGTTCATTATCT
    TTATTACTTTTCAAAAGAAAAGAATCTAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1053

⊟Protein[edit | edit source]

Protein Data Bank: 3QZL

⊟General[edit | edit source]

  • locus tag: NWMN_1041 [new locus tag: NWMN_RS05890 ]
  • symbol: IsdA
  • description: iron-regulated heme-iron binding protein IsdA
  • length: 350
  • theoretical pI: 10.2642
  • theoretical MW: 38745.2
  • GRAVY: -0.817714

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    heme uptake protein IsdC (TIGR03656; HMM-score: 60.1)
    and 3 more
    Cell structure Cell envelope Other LPXTG cell wall anchor domain (TIGR01167; HMM-score: 22.5)
    gram-positive signal peptide, YSIRK family (TIGR01168; HMM-score: 16)
    pullulanase, extracellular (TIGR02102; HMM-score: 9.8)
  • ⊞TheSEED  :
    • Cell surface protein IsdA, transfers heme from hemoglobin to apo-IsdC
    Cell Wall and Capsule Gram-Positive cell wall components Sortase  Cell surface protein IsdA, transfers heme from hemoglobin to apo-IsdC
    and 1 more
    Iron acquisition and metabolism Iron acquisition and metabolism - no subcategory Heme, hemin uptake and utilization systems in GramPositives  Cell surface protein IsdA, transfers heme from hemoglobin to apo-IsdC
  • ⊞PFAM:
    E-set (CL0159) NEAT; Iron Transport-associated domain (PF05031; HMM-score: 103.3)
    and 3 more
    no clan defined Gram_pos_anchor; LPXTG cell wall anchor motif (PF00746; HMM-score: 31.5)
    DUF5044; Domain of unknown function (DUF5044) (PF16447; HMM-score: 15.2)
    post-AAA (CL0604) MgsA_C; MgsA AAA+ ATPase C terminal (PF12002; HMM-score: 7.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cellwall
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 10
    • Extracellular Score: 0
    • Internal Helices: 2
  • ⊞DeepLocPro: Cell wall & surface
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.1292
    • Cell wall & surface Score: 0.7325
    • Extracellular Score: 0.1382
  • ⊞LocateP: LPxTG Cell-wall anchored
    • Prediction by SwissProt Classification: Cell Wall
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0
    • Signal peptide possibility: 0
    • N-terminally Anchored Score: -2
    • Predicted Cleavage Site: found LPxTG motif :LPKTG
  • ⊞SignalP: Signal peptide SP(Sec/SPI) length 46 aa
    • SP(Sec/SPI): 0.847946
    • TAT(Tat/SPI): 0.055607
    • LIPO(Sec/SPII): 0.011802
    • Cleavage Site: CS pos: 46-47. VNA-AT. Pr: 0.7236
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTKHYLNSKYQSEQRSSAMKKITMGTASIILGSLVYIGADSQQVNAATEATNATNNQSTQVSQATSQPINFQVQKDGSSEKSHMDDYMQHPGKVIKQNNKYYFQTVLNNASFWKEYKFYNANNQELATTVVNDNKKADTRTINVAVEPGYKSLTTKVHIVVPQINYNHRYTTHLEFEKAIPTLADAAKPNNVKPVQPKPAQPKTPTEQTKPVQPKVEKVKPTVTTTSKVEDNHSTKVVSTDTTKDQTKTQTAHTVKTAQTAQEQNKVQTPVKDVATAKSESNNQAVSDNKSQQTNKVTKHNETPKQASKAKELPKTGLTSVDNFISTVAFATLALLGSLSLLLFKRKESK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • ⊞regulator: Fur* (repression) regulon

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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