PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA0977 [new locus tag: SA_RS05540 ]
• pan locus tag^?: SAUPAN003359000
• symbol: isdA
• pan gene symbol^?: isdA
• synonym:
• product: cell surface protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA0977 [new locus tag: SA_RS05540 ]
• symbol: isdA
• product: cell surface protein
• replicon: chromosome
• strand: -
• coordinates: 1106798..1107850
• length: 1053
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1123804 NCBI
• RefSeq: NP_374247 NCBI
• BioCyc: see SA_RS05540
• MicrobesOnline: 103273 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  
  ATGACAAAACATTATTTAAACAGTAAGTATCAATCAGAACAACGTTCATCAGCTATGAAA
  AAGATTACAATGGGTACAGCATCTATCATTTTAGGTTCCCTTGTATACATAGGCGCAGAC
  AGCCAACAAGTCAATGCGGCAACAGAAGCTACGAACGCAACTAATAATCAAAGCACACAA
  GTTTCTCAAGCAACATCACAACCAATTAATTTCCAAGTGCAAAAAGATGGCTCTTCAGAG
  AAGTCACACATGGATGACTATATGCAACACCCTGGTAAAGTAATTAAACAAAATAATAAA
  TATTATTTCCAAACCGTGTTAAACAATGCATCATTCTGGAAAGAATACAAATTTTACAAT
  GCAAACAATCAAGAATTAGCAACAACTGTTGTTAACGATAATAAAAAAGCGGATACTAGA
  ACAATCAATGTTGCAGTTGAACCTGGATATAAGAGCTTAACTACTAAAGTACATATTGTC
  GTGCCACAAATTAATTACAATCATAGATATACTACGCATTTGGAATTTGAAAAAGCAATT
  CCTACATTAGCTGACGCAGCAAAACCAAACAATGTTAAACCGGTTCAACCAAAACCAGCT
  CAACCTAAAACACCTACTGAGCAAACTAAACCAGTTCAACCTAAAGTTGAAAAAGTTAAA
  CCTACTGTAACTACAACAAGCAAAGTTGAAGACAATCACTCTACTAAAGTTGTAAGTACT
  GACACAACAAAAGATCAAACTAAAACACAAACTGCTCATACAGTTAAAACAGCACAAACT
  GCTCAAGAACAAAATAAAGTTCAAACACCTGTTAAAGATGTTGCAACAGCGAAATCTGAA
  AGCAACAATCAAGCTGTAAGTGATAATAAATCACAACAAACTAACAAAGTTACAAAACAT
  AACGAAACGCCTAAACAAGCATCTAAAGCTAAAGAATTACCAAAAACTGGTTTAACTTCA
  GTTGATAACTTTATTAGCACAGTTGCCTTCGCAACACTTGCCCTTTTAGGTTCATTATCT
  TTATTACTTTTCAAAAGAAAAGAATCTAAATAA
  60
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  240
  300
  360
  420
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  1020
  1053
  

⊟Protein

• 3QZL
  PDB
• 3QZM
  PDB
• 3QZN
  PDB
• 3QZO
  PDB
• 3QZP
  PDB
• 2ITE
  PDB
• 2ITF
  PDB

Protein Data Bank: 3QZL

Protein Data Bank: 3QZM

Protein Data Bank: 3QZN

Protein Data Bank: 3QZO

Protein Data Bank: 3QZP

Protein Data Bank: 2ITE

Protein Data Bank: 2ITF

⊟General

• locus tag: SA0977 [new locus tag: SA_RS05540 ]
• symbol: IsdA
• description: cell surface protein
• length: 350
• theoretical pI: 10.2642
• theoretical MW: 38745.2
• GRAVY: -0.817714

⊟Function

• TIGRFAM:
  heme uptake protein IsdC (TIGR03656; HMM-score: 60.1)
  and 3 more
  Cell structure Cell envelope Other LPXTG cell wall anchor domain (TIGR01167; HMM-score: 22.5)
  gram-positive signal peptide, YSIRK family (TIGR01168; HMM-score: 16)
  pullulanase, extracellular (TIGR02102; HMM-score: 9.8)
• TheSEED  :
  • Cell surface protein IsdA, transfers heme from hemoglobin to apo-IsdC
  Cell Wall and Capsule Gram-Positive cell wall components Sortase  Cell surface protein IsdA, transfers heme from hemoglobin to apo-IsdC
  and 1 more
  Iron acquisition and metabolism Iron acquisition and metabolism - no subcategory Heme, hemin uptake and utilization systems in GramPositives  Cell surface protein IsdA, transfers heme from hemoglobin to apo-IsdC
• PFAM:
  no clan defined NEAT; Iron Transport-associated domain (PF05031; HMM-score: 87.5)
  and 4 more
  Gram_pos_anchor; LPXTG cell wall anchor motif (PF00746; HMM-score: 37.1)
  DUF5044; Domain of unknown function (DUF5044) (PF16447; HMM-score: 15.3)
  YjbE; Exopolysaccharide production protein YjbE (PF11106; HMM-score: 13)
  post-AAA (CL0604) MgsA_C; MgsA AAA+ ATPase C terminal (PF12002; HMM-score: 6.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cellwall
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 10
  • Extracellular Score: 0
  • Internal Helices: 2
• LocateP: LPxTG Cell-wall anchored
  • Prediction by SwissProt Classification: Cell Wall
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0
  • Signal peptide possibility: 0
  • N-terminally Anchored Score: -2
  • Predicted Cleavage Site: found LPxTG motif :LPKTG
• SignalP: Signal peptide SP(Sec/SPI) length 46 aa
  • SP(Sec/SPI): 0.847946
  • TAT(Tat/SPI): 0.055607
  • LIPO(Sec/SPII): 0.011802
  • Cleavage Site: CS pos: 46-47. VNA-AT. Pr: 0.7236
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 15926714 NCBI
• RefSeq: NP_374247 NCBI
• UniProt: Q7A655 UniProt

⊟Protein sequence

• MTKHYLNSKYQSEQRSSAMKKITMGTASIILGSLVYIGADSQQVNAATEATNATNNQSTQVSQATSQPINFQVQKDGSSEKSHMDDYMQHPGKVIKQNNKYYFQTVLNNASFWKEYKFYNANNQELATTVVNDNKKADTRTINVAVEPGYKSLTTKVHIVVPQINYNHRYTTHLEFEKAIPTLADAAKPNNVKPVQPKPAQPKTPTEQTKPVQPKVEKVKPTVTTTSKVEDNHSTKVVSTDTTKDQTKTQTAHTVKTAQTAQEQNKVQTPVKDVATAKSESNNQAVSDNKSQQTNKVTKHNETPKQASKAKELPKTGLTSVDNFISTVAFATLALLGSLSLLLFKRKESK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: isdB < isdA

⊟Regulation

• regulator: Fur* (repression) regulon

  Fur*

  (TF)

  important in Iron homeostasis; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Simon R Clarke, Michael D Wiltshire, Simon J Foster
IsdA of Staphylococcus aureus is a broad spectrum, iron-regulated adhesin.
Mol Microbiol: 2004, 51(5);1509-19
[PubMed:14982642] [WorldCat.org] [DOI] (P p)Christie L Vermeiren, Mark Pluym, John Mack, David E Heinrichs, Martin J Stillman
Characterization of the heme binding properties of Staphylococcus aureus IsdA.
Biochemistry: 2006, 45(42);12867-75
[PubMed:17042505] [WorldCat.org] [DOI] (P p)Yukiko K Stranger-Jones, Taeok Bae, Olaf Schneewind
Vaccine assembly from surface proteins of Staphylococcus aureus.
Proc Natl Acad Sci U S A: 2006, 103(45);16942-7
[PubMed:17075065] [WorldCat.org] [DOI] (P p)Mark Pluym, Naomi Muryoi, David E Heinrichs, Martin J Stillman
Heme binding in the NEAT domains of IsdA and IsdC of Staphylococcus aureus.
J Inorg Biochem: 2008, 102(3);480-8
[PubMed:18194816] [WorldCat.org] [DOI] (P p)Jason C Grigg, Cherry X Mao, Michael E P Murphy
Iron-coordinating tyrosine is a key determinant of NEAT domain heme transfer.
J Mol Biol: 2011, 413(3);684-98
[PubMed:21893067] [WorldCat.org] [DOI] (I p)