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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0517 [new locus tag: SACOL_RS02615 ]
  • pan locus tag?: SAUPAN002191000
  • symbol: SACOL0517
  • pan gene symbol?: treC
  • synonym:
  • product: alpha-amylase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0517 [new locus tag: SACOL_RS02615 ]
  • symbol: SACOL0517
  • product: alpha-amylase
  • replicon: chromosome
  • strand: +
  • coordinates: 521961..523601
  • length: 1641
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    GTGTCGAAAGAAATAGATTGGAGAAAATCCGTTGTATATCAAATTTATCCTAAGTCGTTT
    AATGATACGACGGGGAATGGTATAGGAGATATCAATGGAATTATAGAAAAATTGGATTAT
    ATCAAGTTATTGGGTGTTGATTATATTTGGTTAACACCAGTGTATGAATCACCGATGAAT
    GATAATGGCTATGATATCAGCAATTATTTAGAAATCAATGAAGACTTTGGAACGATGGAT
    GATTTTGAAAAGTTAATCAAAGTTGCGCATCAAAAAGACTTGAAAGTGATGTTAGATATT
    GTCATTAATCATACGTCGACGGAGCATGAATGGTTTAAAGAAGCCCGTAAATCTAAAGAT
    AACCCTTATAGAGATTATTACTTTTTCAGATCATCTGAAGACGGGCCGCCAACAAATTGG
    CATTCTAAATTCGGTGGTAATGCATGGAAGTATGATTCTGAGACAGATGAATATTATTTA
    CATTTATTTGATGTCAGTCAAGCTGATTTAAATTGGGATAATCCGGAAGTACGTCAATCG
    TTATATCGCATAGTCAATCATTGGATAGACTTCGGCGTTGATGGTTTTCGATTTGATGTC
    ATTAACTTAATTTCTAAAGGTGAATTTAAGGACTCTGACAAAATAGGTAAAGAATTTTAT
    ACGGATGGTCCTAGAGTGCATGAGTTTCTGCATGAATTAAATCGTCAAACGTTTGGTAAC
    ACTGACATGATGACTATAGGAGAAATGTCTTCGACGACGATTGAAAATTGTATTAAGTAT
    ACACAACCAGAACGCCAAGAATTGAATAGTGTTTTTAATTTTCATCATCTAAAGGTTGAT
    TATGTTGATGGTGAAAAGTGGACAAATGCGAAGCTTGATTTTCATAAGTTAAAGGAAATT
    CTGATGCAATGGCAACGAGGTATTTATGACGGTGGCGGATGGAACGCGATTTTCTGGTGT
    AATCATGATCAGCCACGGGTAGTGTCTAGATTTGGTGATGATACGTCGGAAGAGATGAGG
    ATACAAAGTGCTAAAATGTTAGCTATCGCACTGCATATGTTGCAAGGGACGCCATATATT
    TACCAAGGTGAAGAAATTGGTATGACGGACCCACATTTTACATCAATAGCACAATATCGT
    GATGTTGAATCGATTAATGCCTACCATCAGTTGTTAAGTGAAGGGCATGCTGAAGCGGAT
    GTGTTAGCGATTTTAGGACAGAAGTCACGAGACAATTCGAGAACGCCTATGCAATGGAGT
    GATGATGTTAATGCTGGATTTACAGCTGGTAAGCCTTGGATTGATATTTCGGAAAATTAT
    CATCAGGTCAACGTTAGACAAGCACTTCAGAATAAAGAGTCTATTTTCTATACGTATCAA
    AAATTAATACAATTAAGACATACGCATGATATTATTACGTATGGAGACATTGTGCCACGT
    TTTATGGATCATGATCATTTATTTGTTTATGAACGTCATTATAAGAATCAACAATGGCTA
    GTAATTGCGAATTTCTCAGCATCGGCTGTTGATTTGCCAGAAGGATTGGCTAGAGAAGGT
    TGTGTTGTGATTCAAACAGGCACAGTGGAAAATAATACGATAAGCGGGTTTGGTGCAATT
    GTAATCGAAACAAACGCGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1641

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0517 [new locus tag: SACOL_RS02615 ]
  • symbol: SACOL0517
  • description: alpha-amylase
  • length: 546
  • theoretical pI: 4.88774
  • theoretical MW: 63513.4
  • GRAVY: -0.575641

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 3.2.1.93?  ExPASy
    Alpha,alpha-phosphotrehalase Alpha,alpha-trehalose 6-phosphate + H2O = D-glucose + D-glucose 6-phosphate
  • ⊞TIGRFAM:
    alpha,alpha-phosphotrehalase (TIGR02403; EC 3.2.1.93; HMM-score: 816.1)
    and 9 more
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides trehalose synthase (TIGR02456; EC 5.4.99.16; HMM-score: 295.6)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides malto-oligosyltrehalose trehalohydrolase (TIGR02402; EC 3.2.1.141; HMM-score: 81.6)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides malto-oligosyltrehalose synthase (TIGR02401; EC 5.4.99.15; HMM-score: 58.3)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides 1,4-alpha-glucan branching enzyme (TIGR01515; EC 2.4.1.18; HMM-score: 37.5)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides glycogen debranching enzyme GlgX (TIGR02100; EC 3.2.1.-; HMM-score: 34.9)
    sucrose phosphorylase (TIGR03852; EC 2.4.1.7; HMM-score: 32)
    pullulanase, type I (TIGR02104; EC 3.2.1.41; HMM-score: 31.4)
    pullulanase, extracellular (TIGR02102; HMM-score: 20.7)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides glycogen debranching enzyme (TIGR01531; HMM-score: 20.3)
  • TheSEED  :
    • Trehalose-6-phosphate hydrolase (EC 3.2.1.93)
    Carbohydrates Di- and oligosaccharides Trehalose Uptake and Utilization  Trehalose-6-phosphate hydrolase (EC 3.2.1.93)
  • ⊞PFAM:
    TIM_barrel (CL0036) Alpha-amylase; Alpha amylase, catalytic domain (PF00128; HMM-score: 555)
    and 6 more
    hDGE_amylase; Glycogen debranching enzyme, glucanotransferase domain (PF14701; HMM-score: 28)
    GHD (CL0369) Malt_amylase_C; Maltogenic Amylase, C-terminal domain (PF16657; HMM-score: 22.4)
    DUF3459; Domain of unknown function (DUF3459) (PF11941; HMM-score: 21.2)
    SusG_C; Alpha-amylase SusG C-terminal domain (PF23915; HMM-score: 19)
    TIM_barrel (CL0036) MupG_N; 6-phospho-N-acetylmuramidase, N-terminal (PF19200; HMM-score: 15.2)
    GHD (CL0369) Cyc-maltodext_C; Cyclo-malto-dextrinase C-terminal domain (PF10438; HMM-score: 13.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9982
    • Cytoplasmic Membrane Score: 0.0001
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0016
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006136
    • TAT(Tat/SPI): 0.000127
    • LIPO(Sec/SPII): 0.000452
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSKEIDWRKSVVYQIYPKSFNDTTGNGIGDINGIIEKLDYIKLLGVDYIWLTPVYESPMNDNGYDISNYLEINEDFGTMDDFEKLIKVAHQKDLKVMLDIVINHTSTEHEWFKEARKSKDNPYRDYYFFRSSEDGPPTNWHSKFGGNAWKYDSETDEYYLHLFDVSQADLNWDNPEVRQSLYRIVNHWIDFGVDGFRFDVINLISKGEFKDSDKIGKEFYTDGPRVHEFLHELNRQTFGNTDMMTIGEMSSTTIENCIKYTQPERQELNSVFNFHHLKVDYVDGEKWTNAKLDFHKLKEILMQWQRGIYDGGGWNAIFWCNHDQPRVVSRFGDDTSEEMRIQSAKMLAIALHMLQGTPYIYQGEEIGMTDPHFTSIAQYRDVESINAYHQLLSEGHAEADVLAILGQKSRDNSRTPMQWSDDVNAGFTAGKPWIDISENYHQVNVRQALQNKESIFYTYQKLIQLRHTHDIITYGDIVPRFMDHDHLFVYERHYKNQQWLVIANFSASAVDLPEGLAREGCVVIQTGTVENNTISGFGAIVIETNA

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: Cytoplasmic [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • ⊞regulator: CcpA regulon

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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