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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_000408
  • pan locus tag?: SAUPAN002191000
  • symbol: treC
  • pan gene symbol?: treC
  • synonym:
  • product: alpha,alpha-phosphotrehalase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_000408
  • symbol: treC
  • product: alpha,alpha-phosphotrehalase
  • replicon: chromosome
  • strand: +
  • coordinates: 440770..442410
  • length: 1641
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    GTGTCGAAAGAAATAGATTGGAGAAAATCCGTTGTATATCAAATTTATCCTAAGTCGTTT
    AATGATACGACGGGGAATGGTATAGGAGATATCAATGGAATTATAGAAAAATTGGATTAT
    ATCAAGTTATTGGGTGTTGATTATATTTGGTTAACACCAGTGTATGAATCACCGATGAAT
    GATAATGGCTATGATATCAGCAATTATTTAGAAATCAATGAAGACTTTGGAACGATGGAT
    GATTTTGAAAAGTTAATCAAAGTTGCTCATCAAAAAGACTTGAAAGTGATGTTAGATATT
    GTCATTAATCATACGTCGACGGAGCATGAATGGTTTAAAGAAGCCCGTAAATCTAAAGAC
    AACCCTTATAGAGATTATTACTTTTTCAGATCATCTGAAGACGGGCCGCCAACAAATTGG
    CATTCTAAATTCGGTGGTAATGCATGGAAGTTTGATTCTGAGACAGATGAATATTATTTA
    CATTTATTTGATGTCAGTCAAGCTGATTTAAATTGGGATAATCCGGAAGTACGTCAATCG
    TTATATCGCATAGTCAATCATTGGATAGACTTCGGCGTTGATGGTTTTCGATTTGATGTC
    ATTAACTTAATTTCTAAAGGTGAATTTAAGGACTCTGACAAAATAGGTAAAGAATTTTAT
    ACGGATGGTCCTAGAGTGCATGAGTTTCTGCATGAATTAAATCGTCAAACGTTTGGTAAC
    ACTGACATGATGACTGTAGGAGAAATGTCTTCGACGACGATTGAAAATTGTATTAAGTAT
    ACACAGCCAGAACGCCAAGAATTGAATAGTGTTTTTAATTTTCATCATCTAAAGGTTGAT
    TATGTTGATGGTGAAAAGTGGACAAATGCGAAGCTTGATTTCCATAAGTTAAAGGAAATT
    CTGATGCAATGGCAACGAGGTATTTATGACGGTGGCGGATGGAACGCGATTTTTTGGTGT
    AATCATGATCAGCCACGGGTAGTGTCTAGATTTGGTGATGATACGTCGGAAGAGATGAGG
    ATACAAAGTGCTAAAATGTTAGCTATCGCACTGCATATGTTGCAAGGGACGCCATATATT
    TACCAAGGTGAAGAAATTGGTATGACGGACCCACATTTTACATCAATAGCACAATATCGT
    GATGTTGAATCGATTAATGCCTACCATCAGTTGTTAAGTGAAGGGCATGCTGAAGCGGAT
    GTATTAGCGATTTTAGGACAGAAGTCACGAGACAATTCGAGAACGCCTATGCAATGGAGT
    GATGATGTTAATGCTGGATTTACAGCTGGTAAGCCTTGGATTGATATTTCGGAAAATTAT
    CATCAGGTCAACGTTAGACAAGCACTTCAGAATAAAGAGTCTATTTTCTATACGTATCAA
    AAATTAATACAATTAAGACATACGCATGATATTATTACGTATGGAGACATTGTGCCACGT
    TTTATGGATCATGATCATTTATTTGTTTATGAACGTCATTATAAGAATCAACAATGGCTA
    GTAATTGCGAATTTCTCAGCATCGGCGGTTGATTTGCCAGAAGGATTGACTAGCGAAGGT
    CGTGTTGTGATTCAAACAGGCACAGTGGAAAATAATACGATAAGCGGGTTTGGTGCAATT
    GTAATCGAAACAAACGCGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1641

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_000408
  • symbol: TreC
  • description: alpha,alpha-phosphotrehalase
  • length: 546
  • theoretical pI: 4.88774
  • theoretical MW: 63497.4
  • GRAVY: -0.579304

⊟Function[edit | edit source]

  • reaction:
    EC 3.2.1.93?  ExPASy
    Alpha,alpha-phosphotrehalase Alpha,alpha-trehalose 6-phosphate + H2O = D-glucose + D-glucose 6-phosphate
  • TIGRFAM:
    alpha,alpha-phosphotrehalase (TIGR02403; EC 3.2.1.93; HMM-score: 816.8)
    and 9 more
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides trehalose synthase (TIGR02456; EC 5.4.99.16; HMM-score: 295)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides malto-oligosyltrehalose trehalohydrolase (TIGR02402; EC 3.2.1.141; HMM-score: 81.2)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides malto-oligosyltrehalose synthase (TIGR02401; EC 5.4.99.15; HMM-score: 57.9)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides 1,4-alpha-glucan branching enzyme (TIGR01515; EC 2.4.1.18; HMM-score: 37.4)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides glycogen debranching enzyme GlgX (TIGR02100; EC 3.2.1.-; HMM-score: 36.5)
    sucrose phosphorylase (TIGR03852; EC 2.4.1.7; HMM-score: 32)
    pullulanase, type I (TIGR02104; EC 3.2.1.41; HMM-score: 31.5)
    pullulanase, extracellular (TIGR02102; HMM-score: 20.7)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides glycogen debranching enzyme (TIGR01531; HMM-score: 20.3)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    TIM_barrel (CL0036) Alpha-amylase; Alpha amylase, catalytic domain (PF00128; HMM-score: 555.7)
    and 6 more
    hDGE_amylase; Glycogen debranching enzyme, glucanotransferase domain (PF14701; HMM-score: 28)
    GHD (CL0369) DUF3459; Domain of unknown function (DUF3459) (PF11941; HMM-score: 23.9)
    Malt_amylase_C; Maltogenic Amylase, C-terminal domain (PF16657; HMM-score: 22)
    SusG_C; Alpha-amylase SusG C-terminal domain (PF23915; HMM-score: 20)
    TIM_barrel (CL0036) MupG_N; 6-phospho-N-acetylmuramidase, N-terminal (PF19200; HMM-score: 15.4)
    GHD (CL0369) Cyc-maltodext_C; Cyclo-malto-dextrinase C-terminal domain (PF10438; HMM-score: 13.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9982
    • Cytoplasmic Membrane Score: 0.0001
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0016
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006136
    • TAT(Tat/SPI): 0.000127
    • LIPO(Sec/SPII): 0.000452
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MSKEIDWRKSVVYQIYPKSFNDTTGNGIGDINGIIEKLDYIKLLGVDYIWLTPVYESPMNDNGYDISNYLEINEDFGTMDDFEKLIKVAHQKDLKVMLDIVINHTSTEHEWFKEARKSKDNPYRDYYFFRSSEDGPPTNWHSKFGGNAWKFDSETDEYYLHLFDVSQADLNWDNPEVRQSLYRIVNHWIDFGVDGFRFDVINLISKGEFKDSDKIGKEFYTDGPRVHEFLHELNRQTFGNTDMMTVGEMSSTTIENCIKYTQPERQELNSVFNFHHLKVDYVDGEKWTNAKLDFHKLKEILMQWQRGIYDGGGWNAIFWCNHDQPRVVSRFGDDTSEEMRIQSAKMLAIALHMLQGTPYIYQGEEIGMTDPHFTSIAQYRDVESINAYHQLLSEGHAEADVLAILGQKSRDNSRTPMQWSDDVNAGFTAGKPWIDISENYHQVNVRQALQNKESIFYTYQKLIQLRHTHDIITYGDIVPRFMDHDHLFVYERHYKNQQWLVIANFSASAVDLPEGLTSEGRVVIQTGTVENNTISGFGAIVIETNA

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: CcpA regulon, TreR (repression) regulon
    CcpA(TF)important in Carbon catabolism;  regulation predicted or transferred from N315 and NCTC 8325  [2]
    TreR(TF)important in Trehalose utilization;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ 2.0 2.1 Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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