⊟Summary[edit | edit source]

PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
locus tag: SACOL_RS07835 [old locus tag: SACOL1537 ]
pan locus tag^?: SAUPAN004027000
symbol: SACOL_RS07835
pan gene symbol^?: scpB
• synonym:
• product: SMC-Scp complex subunit ScpB

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS07835 [old locus tag: SACOL1537 ]
• symbol: SACOL_RS07835
• product: SMC-Scp complex subunit ScpB
• replicon: chromosome
• strand: -
• coordinates: 1575673..1576215
• length: 543
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (1575673..1576215) NCBI
• BioCyc: SACOL_RS07835 BioCyc
• MicrobesOnline: see SACOL1537

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  
  TTGGATAATCATGGTATATTAGAGTCGCTTTTATTTACAGCTGGCGATGAAGGTTTAGAT
  GAAAAACAACTATTAGAAATATTAGATATGTCGAAAGACCAACTCGTTGAATTAATTGAA
  AATTATTCATCACATGGATTAATGATACAACGATTTGGAATGACGTATGTTTTAACGACT
  AAAAAAGAAGCGGCAACGTATATTGAACAATTAATTGAACAAAAGTCACAAATGAAATTA
  TCACAAGCAGCAATGGAAGTACTATCAATTATTGCTTATAACCAGCCATTATCAAGAAGT
  GATATTGAATTAATTCGTAGTATCAATTCAGATGGTGCAGTTAAGACATTGATTGCCAAA
  GGACTAGTTGAGGCTAAAGTGGTTAATGAACAGCGTAGCCAACAGTTAATTACTACTGAT
  TTATTTTTAAATGTATTTGGTATTTCCAATATAGAAGATTTGCCGACAACTGAAGAAGAC
  GATGAAGAAATGGATGCTTTTTTCAGTAATCTAGTCAATCAAAAAGGAGAAAATAATGAC
  TAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  543
  

⊟Protein

⊟General

• locus tag: SACOL_RS07835 [old locus tag: SACOL1537 ]
• symbol: SACOL_RS07835
• description: SMC-Scp complex subunit ScpB
• length: 180
• theoretical pI: 4.03334
• theoretical MW: 20235.7
• GRAVY: -0.251667

⊟Function

• TIGRFAM:
  Cellular processes Cellular processes Cell division segregation and condensation protein B (TIGR00281; HMM-score: 122.4)
  Genetic information processing DNA metabolism Chromosome-associated proteins segregation and condensation protein B (TIGR00281; HMM-score: 122.4)
• TheSEED: see SACOL1537
• ⊞⊞PFAM:
  HTH (CL0123) SMC_ScpB; Segregation and condensation complex subunit ScpB (PF04079; HMM-score: 144.7)
  and 3 more
  MarR_2; MarR family (PF12802; HMM-score: 16.5)
  no clan defined NYD-SP12_N; Spermatogenesis-associated, N-terminal (PF15015; HMM-score: 14.6)
  Sigma54_AID; Sigma-54 factor, Activator interacting domain (AID) (PF00309; HMM-score: 7.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.67
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 0.15
  • Extracellular Score: 0.17
  • Internal Helices: 0
• ⊞⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9859
  • Cytoplasmic Membrane Score: 0.0088
  • Cell wall & surface Score: 0.0004
  • Extracellular Score: 0.0049
• LocateP:
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003773
  • TAT(Tat/SPI): 0.000246
  • LIPO(Sec/SPII): 0.000358
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446290798 NCBI
• RefSeq: WP_000368653 NCBI
• UniProt: see SACOL1537

⊟Protein sequence

• MDNHGILESLLFTAGDEGLDEKQLLEILDMSKDQLVELIENYSSHGLMIQRFGMTYVLTTKKEAATYIEQLIEQKSQMKLSQAAMEVLSIIAYNQPLSRSDIELIRSINSDGAVKTLIAKGLVEAKVVNEQRSQQLITTDLFLNVFGISNIEDLPTTEEDDEEMDAFFSNLVNQKGENND

⊟Experimental data

• experimentally validated: see SACOL1537
• protein localization: see SACOL1537
• quantitative data / protein copy number per cell: see SACOL1537
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications