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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1031 [new locus tag: SA_RS05870 ]
  • pan locus tag?: SAUPAN003459000
  • symbol: SA1031
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1031 [new locus tag: SA_RS05870 ]
  • symbol: SA1031
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1168023..1168697
  • length: 675
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    TTGCGTGTGAAAGATAATTTACAACAAATCTCAACACAAATTAATGACAAAAGTGAAAAA
    AATAATTTTTCAACAAAACCAAACGTGATTGCAGTTACAAAATATGTTACAATAGAGCGA
    GCTAAAGAAGCGTATGAGGCTGGAATAAGACATTTTGGTGAGAATAGATTGGAAGGCTTT
    TTTCAAAAGAAAGAAGCATTACCATCAGATGCGGTGATTCATTTTATAGGATCATTACAA
    TCTCGAAAAGTTAAGGACGTTATAAACGACGTAGATTATTTCCATGCTTTAGATCGATTG
    AGCTTAGCCAAAGAAATTAACAAACGTGCAGAACATAAAATTAAATGTTTCTTGCAAGTG
    AACGTTTCGGGAGAAGCTTCTAAACATGGTATTGCTTTAGAAGATGTTGATCAGTTTATA
    GATGATCTTAAAAAATATGACAAAATCGAAATTGTAGGTTTAATGACGATGGCACCATTG
    ACAGATGATGAAGCATATATTAGATCGTTATTTAAACAGTTACGTTTGAAAAAAGAAGAA
    ATACAACGACTCAATTTAGAATATGCGCCTTGTGATGAATTATCAATGGGAATGAGTAAT
    GACTATCTTATTGCAGTTGAAGAAGGTGCGACGTTTGTTAGAATTGGGACTAAACTTGTA
    GGAGAAGAGGAGTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    675

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1031 [new locus tag: SA_RS05870 ]
  • symbol: SA1031
  • description: hypothetical protein
  • length: 224
  • theoretical pI: 5.73426
  • theoretical MW: 25602.1
  • GRAVY: -0.437946

⊟Function[edit | edit source]

  • TIGRFAM:
    Unknown function Enzymes of unknown specificity pyridoxal phosphate enzyme, YggS family (TIGR00044; HMM-score: 158.3)
  • TheSEED  :
    • Pyridoxal phosphate homeostasis protein YggS
    Amino Acids and Derivatives Proline and 4-hydroxyproline A Hypothetical Protein Related to Proline Metabolism  Hypothetical protein YggS, proline synthase co-transcribed bacterial homolog PROSC
  • PFAM:
    TIM_barrel (CL0036) Ala_racemase_N; Alanine racemase, N-terminal domain (PF01168; HMM-score: 71.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: pyridoxal 5'-phosphate
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9985
    • Cytoplasmic Membrane Score: 0.0001
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0014
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.008491
    • TAT(Tat/SPI): 0.000862
    • LIPO(Sec/SPII): 0.001328
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MRVKDNLQQISTQINDKSEKNNFSTKPNVIAVTKYVTIERAKEAYEAGIRHFGENRLEGFFQKKEALPSDAVIHFIGSLQSRKVKDVINDVDYFHALDRLSLAKEINKRAEHKIKCFLQVNVSGEASKHGIALEDVDQFIDDLKKYDKIEIVGLMTMAPLTDDEAYIRSLFKQLRLKKEEIQRLNLEYAPCDELSMGMSNDYLIAVEEGATFVRIGTKLVGEEE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • ⊟interaction partners:
    SA0562(adh1)alcohol dehydrogenase  [2] (data from MRSA252)
    SA0506(tuf)elongation factor Tu  [2] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ R Allyn Forsyth, Robert J Haselbeck, Kari L Ohlsen, Robert T Yamamoto, Howard Xu, John D Trawick, Daniel Wall, Liangsu Wang, Vickie Brown-Driver, Jamie M Froelich, Kedar G C, Paula King, Melissa McCarthy, Cheryl Malone, Brian Misiner, David Robbins, Zehui Tan, Zhan-yang Zhu Zy, Grant Carr, Deborah A Mosca, Carlos Zamudio, J Gordon Foulkes, Judith W Zyskind
    A genome-wide strategy for the identification of essential genes in Staphylococcus aureus.
    Mol Microbiol: 2002, 43(6);1387-400
    [PubMed:11952893] [WorldCat.org] [DOI] (P p)
  2. ↑ Jump up to: 2.0 2.1 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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