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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0803 [new locus tag: SA_RS04580 ]
  • pan locus tag?: SAUPAN003041000
  • symbol: ampA
  • pan gene symbol?: pepZ
  • synonym:
  • product: cytosol aminopeptidase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA0803 [new locus tag: SA_RS04580 ]
  • symbol: ampA
  • product: cytosol aminopeptidase
  • replicon: chromosome
  • strand: +
  • coordinates: 907312..908787
  • length: 1476
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATA
    CCAGAACATTTAAATCAGTTAGAGCGCATTAGTTTTAATCATATCGATATTACAGAATCA
    CTTGAAAGACTAAAACATCAACATATTATTGGTAGTAAAGTTGGGAAGATTTATACAACT
    GCATTTGATGTACAAGATCAAACATATCGTTTAATTACAGTTGGTTTAGGAAACTTAAAA
    GCACGTAGTTATCAAGATATGTTGAAAATATGGGGACATCTTTTCCAATACATAAAGTCA
    GAACATATTGAAGATACTTATTTACTTATGGATTCATTTATTTCAAAATATGATCAGTTA
    TCAGATGTATTAATGGCATGCGGTATTCAAAGTGAGCGTGCAACATATGAATTCGATCAT
    TATAAATCAAGTAAGAAGGCACCGTTTAAGACGAATTTAAACCTTATTAGTGAATCATTA
    ATTGAATTAGATTTCATTCATGAGGGTATCAGTATTGGCCAATCCATTAATTTGGCAAGA
    GACTTTAGTAATATGCCACCGAATGTATTAACACCACAAACATTTGCAGAAGATATTGTT
    AATCATTTTAAAAATACAAAGGTCAAAGTAGATGTTAAAGATTATGACACTTTAGTTTCT
    GAAGGATTCGGACTTTTACAAGCAGTAGGTAAAGGTAGTAAGCATAAACCGAGATTAGTA
    ACCATCACATATAATGGCAAAGACAAAGATGTAGCACCAATTGCCTTAGTTGGTAAAGGT
    ATAACGTATGATTCTGGTGGTTATAGTATTAAAACGAAGAATGGCATGGCTACAATGAAG
    TTTGACATGTGTGGCGCTGCGAATGTCGTTGGTATCATTGAAGCGGCTAGTCGTTTACAA
    CTGCCTGTAAATATTGTCGGAGTGCTTGCTTGTGCTGAAAATATGATAAATGAAGCATCA
    ATGAAGCCAGATGATGTATTTACAGCATTAAGTGGTGAAACTGTAGAAGTAATGAATACA
    GACGCTGAAGGTAGATTAGTCCTTGCAGATGCTGTGTATTATGCAAATCAATATCAGCCT
    AGTGTGATTATGGACTTTGCTACATTAACGGGTGCAGCCATCGTTGCACTAGGCGATGAT
    AAAGCTGCTGCATTTGAATCGAATAGTAAAGTGATATTAAACGATATATTACAAATAAGT
    TCTAAAGTCGATGAAATGGTATTTGAATTACCGATTACTGCAACCGAACGTGCAAGTATT
    AATCACAGTGATATCGCTGATTTAGTTAACCATACGAATGGACAAGGTAAAGCGCTATTT
    GCGGCAAGTTTTGTAACACATTTTAGTGGTCAAACACCTCACATTCATTTCGATATTGCA
    GGTCCAGCAACGACTAATAAAGCTTCATATAATGGTCCAAAAGGGCCAACAGGATTTATG
    ATTCCGACGATAGTACAATGGTTAAAACAACAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1476

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA0803 [new locus tag: SA_RS04580 ]
  • symbol: AmpA
  • description: cytosol aminopeptidase
  • length: 491
  • theoretical pI: 5.9906
  • theoretical MW: 54069.3
  • GRAVY: -0.104481

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 3.4.11.1  ExPASy
    Leucyl aminopeptidase Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low?
  • TIGRFAM:
  • ⊞TheSEED  :
    • Cytosol aminopeptidase PepA (EC 3.4.11.1)
    Carbohydrates Central carbohydrate metabolism Dehydrogenase complexes  Cytosol aminopeptidase PepA (EC 3.4.11.1)
    and 1 more
    Protein Metabolism Protein degradation EC 3.4.11.- Aminopeptidases  Cytosol aminopeptidase PepA (EC 3.4.11.1)
  • ⊞PFAM:
    ZnExoPePases (CL0865) Peptidase_M17; Cytosol aminopeptidase family, catalytic domain (PF00883; HMM-score: 361.6)
    and 1 more
    MACRO (CL0223) Peptidase_M17_N; Cytosol aminopeptidase family, N-terminal domain (PF02789; HMM-score: 75.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mn2+
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9329
    • Cytoplasmic Membrane Score: 0.0231
    • Cell wall & surface Score: 0.0106
    • Extracellular Score: 0.0333
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006708
    • TAT(Tat/SPI): 0.000118
    • LIPO(Sec/SPII): 0.000244
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNFKLNNTLSNEINTLIIGIPEHLNQLERISFNHIDITESLERLKHQHIIGSKVGKIYTTAFDVQDQTYRLITVGLGNLKARSYQDMLKIWGHLFQYIKSEHIEDTYLLMDSFISKYDQLSDVLMACGIQSERATYEFDHYKSSKKAPFKTNLNLISESLIELDFIHEGISIGQSINLARDFSNMPPNVLTPQTFAEDIVNHFKNTKVKVDVKDYDTLVSEGFGLLQAVGKGSKHKPRLVTITYNGKDKDVAPIALVGKGITYDSGGYSIKTKNGMATMKFDMCGAANVVGIIEAASRLQLPVNIVGVLACAENMINEASMKPDDVFTALSGETVEVMNTDAEGRLVLADAVYYANQYQPSVIMDFATLTGAAIVALGDDKAAAFESNSKVILNDILQISSKVDEMVFELPITATERASINHSDIADLVNHTNGQGKALFAASFVTHFSGQTPHIHFDIAGPATTNKASYNGPKGPTGFMIPTIVQWLKQQ

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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