PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA0803 [new locus tag: SA_RS04580 ]
• pan locus tag^?: SAUPAN003041000
• symbol: ampA
• pan gene symbol^?: pepZ
• synonym:
• product: cytosol aminopeptidase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA0803 [new locus tag: SA_RS04580 ]
• symbol: ampA
• product: cytosol aminopeptidase
• replicon: chromosome
• strand: +
• coordinates: 907312..908787
• length: 1476
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1123618 NCBI
• RefSeq: NP_374064 NCBI
• BioCyc: see SA_RS04580
• MicrobesOnline: 103090 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATA
  CCAGAACATTTAAATCAGTTAGAGCGCATTAGTTTTAATCATATCGATATTACAGAATCA
  CTTGAAAGACTAAAACATCAACATATTATTGGTAGTAAAGTTGGGAAGATTTATACAACT
  GCATTTGATGTACAAGATCAAACATATCGTTTAATTACAGTTGGTTTAGGAAACTTAAAA
  GCACGTAGTTATCAAGATATGTTGAAAATATGGGGACATCTTTTCCAATACATAAAGTCA
  GAACATATTGAAGATACTTATTTACTTATGGATTCATTTATTTCAAAATATGATCAGTTA
  TCAGATGTATTAATGGCATGCGGTATTCAAAGTGAGCGTGCAACATATGAATTCGATCAT
  TATAAATCAAGTAAGAAGGCACCGTTTAAGACGAATTTAAACCTTATTAGTGAATCATTA
  ATTGAATTAGATTTCATTCATGAGGGTATCAGTATTGGCCAATCCATTAATTTGGCAAGA
  GACTTTAGTAATATGCCACCGAATGTATTAACACCACAAACATTTGCAGAAGATATTGTT
  AATCATTTTAAAAATACAAAGGTCAAAGTAGATGTTAAAGATTATGACACTTTAGTTTCT
  GAAGGATTCGGACTTTTACAAGCAGTAGGTAAAGGTAGTAAGCATAAACCGAGATTAGTA
  ACCATCACATATAATGGCAAAGACAAAGATGTAGCACCAATTGCCTTAGTTGGTAAAGGT
  ATAACGTATGATTCTGGTGGTTATAGTATTAAAACGAAGAATGGCATGGCTACAATGAAG
  TTTGACATGTGTGGCGCTGCGAATGTCGTTGGTATCATTGAAGCGGCTAGTCGTTTACAA
  CTGCCTGTAAATATTGTCGGAGTGCTTGCTTGTGCTGAAAATATGATAAATGAAGCATCA
  ATGAAGCCAGATGATGTATTTACAGCATTAAGTGGTGAAACTGTAGAAGTAATGAATACA
  GACGCTGAAGGTAGATTAGTCCTTGCAGATGCTGTGTATTATGCAAATCAATATCAGCCT
  AGTGTGATTATGGACTTTGCTACATTAACGGGTGCAGCCATCGTTGCACTAGGCGATGAT
  AAAGCTGCTGCATTTGAATCGAATAGTAAAGTGATATTAAACGATATATTACAAATAAGT
  TCTAAAGTCGATGAAATGGTATTTGAATTACCGATTACTGCAACCGAACGTGCAAGTATT
  AATCACAGTGATATCGCTGATTTAGTTAACCATACGAATGGACAAGGTAAAGCGCTATTT
  GCGGCAAGTTTTGTAACACATTTTAGTGGTCAAACACCTCACATTCATTTCGATATTGCA
  GGTCCAGCAACGACTAATAAAGCTTCATATAATGGTCCAAAAGGGCCAACAGGATTTATG
  ATTCCGACGATAGTACAATGGTTAAAACAACAATAA
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⊟Protein

⊟General

• locus tag: SA0803 [new locus tag: SA_RS04580 ]
• symbol: AmpA
• description: cytosol aminopeptidase
• length: 491
• theoretical pI: 5.9906
• theoretical MW: 54069.3
• GRAVY: -0.104481

⊟Function

• reaction:
  EC 3.4.11.1  ExPASy
  Leucyl aminopeptidase Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low?
• TIGRFAM:
• TheSEED  :
  • Cytosol aminopeptidase PepA (EC 3.4.11.1)
  Carbohydrates Central carbohydrate metabolism Dehydrogenase complexes  Cytosol aminopeptidase PepA (EC 3.4.11.1)
  and 1 more
  Protein Metabolism Protein degradation EC 3.4.11.- Aminopeptidases  Cytosol aminopeptidase PepA (EC 3.4.11.1)
• PFAM:
  Peptidase_MH (CL0035) Peptidase_M17; Cytosol aminopeptidase family, catalytic domain (PF00883; HMM-score: 356.3)
  and 3 more
  MACRO (CL0223) Peptidase_M17_N; Cytosol aminopeptidase family, N-terminal domain (PF02789; HMM-score: 62.6)
  no clan defined FliS_cochap; Flagellar FLiS export co-chaperone, HP1076 (PF16522; HMM-score: 12.6)
  TerD; TerD domain (PF02342; HMM-score: 12.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: Mn²⁺
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.67
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 0.15
  • Extracellular Score: 0.17
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.006708
  • TAT(Tat/SPI): 0.000118
  • LIPO(Sec/SPII): 0.000244
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15926531 NCBI
• RefSeq: NP_374064 NCBI
• UniProt: A0A0H3JPD1 UniProt

⊟Protein sequence

• MNFKLNNTLSNEINTLIIGIPEHLNQLERISFNHIDITESLERLKHQHIIGSKVGKIYTTAFDVQDQTYRLITVGLGNLKARSYQDMLKIWGHLFQYIKSEHIEDTYLLMDSFISKYDQLSDVLMACGIQSERATYEFDHYKSSKKAPFKTNLNLISESLIELDFIHEGISIGQSINLARDFSNMPPNVLTPQTFAEDIVNHFKNTKVKVDVKDYDTLVSEGFGLLQAVGKGSKHKPRLVTITYNGKDKDVAPIALVGKGITYDSGGYSIKTKNGMATMKFDMCGAANVVGIIEAASRLQLPVNIVGVLACAENMINEASMKPDDVFTALSGETVEVMNTDAEGRLVLADAVYYANQYQPSVIMDFATLTGAAIVALGDDKAAAFESNSKVILNDILQISSKVDEMVFELPITATERASINHSDIADLVNHTNGQGKALFAASFVTHFSGQTPHIHFDIAGPATTNKASYNGPKGPTGFMIPTIVQWLKQQ

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SA0802 > ampA

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications