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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

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⊟Summary[edit | edit source]

Contents

  • 1 Summary
  • 2 Genome View
  • 3 Gene
    • 3.1 General
    • 3.2 Accession numbers
    • 3.3 Phenotype
    • 3.4 DNA sequence
  • 4 Protein
    • 4.1 General
    • 4.2 Function
    • 4.3 Structure, modifications & cofactors
    • 4.4 Localization
    • 4.5 Accession numbers
    • 4.6 Protein sequence
    • 4.7 Experimental data
  • 5 Expression & Regulation
    • 5.1 Operon
    • 5.2 Regulation
    • 5.3 Transcription pattern
    • 5.4 Protein synthesis (provided by Aureolib)
    • 5.5 Protein stability
  • 6 Biological Material
    • 6.1 Mutants
    • 6.2 Expression vector
    • 6.3 lacZ fusion
    • 6.4 GFP fusion
    • 6.5 two-hybrid system
    • 6.6 FLAG-tag construct
    • 6.7 Antibody
  • 7 Other Information
  • 8 Literature
    • 8.1 References
    • 8.2 Relevant publications
  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS02220 [old locus tag: SA0387 ]
  • pan locus tag?: SAUPAN002113000
  • symbol: SA_RS02220
  • pan gene symbol?: ssl7
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS02220 [old locus tag: SA0387 ]
  • symbol: SA_RS02220
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 446880..447575
  • length: 696
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (446880..447575) NCBI
  • BioCyc: SA_RS02220 BioCyc
  • MicrobesOnline: see SA0387

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAAATTAAAAACGTTAGCTAAAGCAACATTGGCATTAGGCTTATTAACTACTGGTGTG
    ATTACATCAGAAGGCCAAGCAGTCCACGCAAAAGAAAAGCAAGAGAGAGTACAACATTTA
    TATGATATTAAAGACTTATATCGATACTACTCATCAGAAAGTTTTGAATTCAGTAATATT
    AGTGGTAAGGTTGAAAACTATAACGGTTCTAACGTTGTACGCTTTAACCAAGAAAAACAA
    AATCACCAATTATTCTTATTAGGAAAAGATAAAGATAAATATAAAAAAGGCCTTGAAGGC
    CAGAATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGACTATCTACTGTTGGT
    GGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTTAATAAA
    GTGTATGGCGGAAATTTAGATGCATCAATTGACTCATTTTTAATTAATAAAGAAGAAGTT
    TCACTGAAAGAACTTGATTTCAAAATTAGAAAGCAATTAGTTGAAAAATATGGTTTATAT
    AAAGGTACGACTAAATACGGTAAGATCACTATCAATTTGAAAGACGAGAAAAAGGAAGTA
    ATTGATTTAGGTGATAAACTGCAATTCGAGCGCATGGGTGATGTGTTGAATAGTAAGGAT
    ATTCAAAATATAGCAGTGACTATTAATCAAATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    696

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS02220 [old locus tag: SA0387 ]
  • symbol: SA_RS02220
  • description: hypothetical protein
  • length: 231
  • theoretical pI: 9.79031
  • theoretical MW: 26202.7
  • GRAVY: -0.580519

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED: see SA0387
  • ⊞PFAM:
    OB_enterotoxin (CL0658) SSL_OB; Staphylococcal superantigen-like OB-fold domain (PF09199; HMM-score: 101.2)
    Ubiquitin (CL0072) Stap_Strp_tox_C; Staphylococcal/Streptococcal toxin, beta-grasp domain (PF02876; HMM-score: 95.6)
    and 2 more
    no clan defined MAP; MAP domain (PF03642; HMM-score: 15.2)
    SH3 (CL0010) BAH; BAH domain (PF01426; HMM-score: 13.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 10
    • Internal Helices: 0
  • ⊞DeepLocPro: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.0018
    • Cell wall & surface Score: 0.0864
    • Extracellular Score: 0.9118
  • LocateP:
  • ⊞SignalP: Signal peptide SP(Sec/SPI) length 30 aa
    • SP(Sec/SPI): 0.988663
    • TAT(Tat/SPI): 0.002021
    • LIPO(Sec/SPII): 0.005649
    • Cleavage Site: CS pos: 30-31. VHA-KE. Pr: 0.8600
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI: 446692474 NCBI
  • RefSeq: WP_000769820 NCBI
  • UniProt: see SA0387

⊟Protein sequence[edit | edit source]

  • MKLKTLAKATLALGLLTTGVITSEGQAVHAKEKQERVQHLYDIKDLYRYYSSESFEFSNISGKVENYNGSNVVRFNQEKQNHQLFLLGKDKDKYKKGLEGQNVFVVKELIDPNGRLSTVGGVTKKNNKSSETNTHLFVNKVYGGNLDASIDSFLINKEEVSLKELDFKIRKQLVEKYGLYKGTTKYGKITINLKDEKKEVIDLGDKLQFERMGDVLNSKDIQNIAVTINQI

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SaeR see SA0387

⊟Transcription pattern[edit | edit source]

  • S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

  • Aureolib: no data available

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Stefan Monecke, Peter Slickers, Ralf Ehricht
Assignment of Staphylococcus aureus isolates to clonal complexes based on microarray analysis and pattern recognition.
FEMS Immunol Med Microbiol: 2008, 53(2);237-51
[PubMed:18507678] [WorldCat.org] [DOI] (P p)

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SA_RS02220&oldid=79394"
  • This page was last edited on 11 March 2016, at 01:30.
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