⊟Summary[edit | edit source]

PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
locus tag: SACOL_RS13955 [old locus tag: SACOL2664 ]
pan locus tag^?: SAUPAN006373000
symbol: SACOL_RS13955
pan gene symbol^?: pmi
• synonym:
• product: mannose-6-phosphate isomerase, class I

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS13955 [old locus tag: SACOL2664 ]
• symbol: SACOL_RS13955
• product: mannose-6-phosphate isomerase, class I
• replicon: chromosome
• strand: +
• coordinates: 2728203..2729141
• length: 939
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (2728203..2729141) NCBI
• BioCyc: SACOL_RS13955 BioCyc
• MicrobesOnline: see SACOL2664

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  
  ATGGCATTATTTTTACAACCCGTTTTTAAAGACAGAATTTGGGGCGGTCATGCATTAAAA
  GCATTTAACTATGATATCCCTAATGAAACAACTGGTGAATGCTGGGCAATTTCTGCACAT
  CCAAATGGTCCTAATACAATTATCAATGGTCCATACAAGGATATGACCCTAGATCAACTT
  TGGTCACAACATCGTGAATTATTCGATAATGATTCACGAGATAGTTTCCCTCTGCTAACT
  AAAGTATTAGATGCCAATGATAAATTATCTGTTCAAGTTCACCCAGATGATGACTACGCT
  TTAAAACACGAAGGCGAACTAGGTAAAACAGAATGTTGGTATATTTTAGATGCAGCCCCA
  GGTGCTGAAATTATATATGGCGTACATGCACATAATAAACAGACGTTAATAGACATGATT
  GATAATCATGAATTTGACAGTCTTTTCAAACGTATACCTGTTAAGCCCGGTGACTTTTAT
  TATGTTCCTGCTGGCACTGTCCATGCCATTGGTTCGGGTATTTTAATTTTAGAGACACAA
  CAATCCTCCGATACGACATACCGTATTTATGACTATGATAGACGAGATCAAAATGGTCAA
  TTGCGTGATTTGCACTTAGAGCAAAGTAAAGATGTGATTGAATTAGGCAATCATGAACCA
  AATACTACTCCTATAACAACTCACATCGACACACACACTGTAACTCAATTTGTAGATAAT
  CAATATTTTGCAGTATATAAATGGGATATTCATGGAGATTTAAAATTCAATAAACCACAT
  GCTTATTGCTTAGTTACAATTATCGATGGTACTGGTACTTTAACAGTAAACGATAAGCAC
  TATGACATCCAAAAAGGTTCTAGCTTTATTTTAACTACGGAAGACGAGGATATTCACTTT
  GTCGGTAATATTAGTGCTATAGTAAGTCATCCTGGATAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  939
  

⊟Protein

⊟General

• locus tag: SACOL_RS13955 [old locus tag: SACOL2664 ]
• symbol: SACOL_RS13955
• description: mannose-6-phosphate isomerase, class I
• length: 312
• theoretical pI: 4.94021
• theoretical MW: 35395.2
• GRAVY: -0.461538

⊟Function

• ⊞⊞reaction:
  EC 5.3.1.8?  ExPASy
  Mannose-6-phosphate isomerase D-mannose 6-phosphate = D-fructose 6-phosphate
• TIGRFAM:
  Metabolism Energy metabolism Sugars mannose-6-phosphate isomerase, class I (TIGR00218; EC 5.3.1.8; HMM-score: 226.2)
• TheSEED: see SACOL2664
• ⊞⊞PFAM:
  Cupin (CL0029) PMI_typeI_cat; Phosphomannose isomerase type I, catalytic domain (PF20511; HMM-score: 92.1)
  MPI_cupin_dom; Mannose-6-phosphate isomerase, cupin domain (PF21621; HMM-score: 80.4)
  and 4 more
  Cupin_2; Cupin domain (PF07883; HMM-score: 35.9)
  AraC_binding; AraC-like ligand binding domain (PF02311; HMM-score: 24.6)
  no clan defined YjbF; Group 4 capsule polysaccharide lipoprotein gfcB, YjbF (PF11102; HMM-score: 12.9)
  Cupin (CL0029) Cupin_3; EutQ-like cupin domain (PF05899; HMM-score: 12.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: Zn²⁺
• effectors:

⊟Localization

• ⊞⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• ⊞⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9976
  • Cytoplasmic Membrane Score: 0.0005
  • Cell wall & surface Score: 0
  • Extracellular Score: 0.0019
• LocateP:
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.050223
  • TAT(Tat/SPI): 0.002634
  • LIPO(Sec/SPII): 0.008036
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 447095945 NCBI
• RefSeq: WP_001173201 NCBI
• UniProt: see SACOL2664

⊟Protein sequence

• MALFLQPVFKDRIWGGHALKAFNYDIPNETTGECWAISAHPNGPNTIINGPYKDMTLDQLWSQHRELFDNDSRDSFPLLTKVLDANDKLSVQVHPDDDYALKHEGELGKTECWYILDAAPGAEIIYGVHAHNKQTLIDMIDNHEFDSLFKRIPVKPGDFYYVPAGTVHAIGSGILILETQQSSDTTYRIYDYDRRDQNGQLRDLHLEQSKDVIELGNHEPNTTPITTHIDTHTVTQFVDNQYFAVYKWDIHGDLKFNKPHAYCLVTIIDGTGTLTVNDKHYDIQKGSSFILTTEDEDIHFVGNISAIVSHPG

⊟Experimental data

• experimentally validated: see SACOL2664
• protein localization: see SACOL2664
• quantitative data / protein copy number per cell: see SACOL2664
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: CcpA see SACOL2664

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications