From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS13955 [old locus tag: SACOL2664 ]
  • pan locus tag?: SAUPAN006373000
  • symbol: SACOL_RS13955
  • pan gene symbol?: pmi
  • synonym:
  • product: mannose-6-phosphate isomerase, class I

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS13955 [old locus tag: SACOL2664 ]
  • symbol: SACOL_RS13955
  • product: mannose-6-phosphate isomerase, class I
  • replicon: chromosome
  • strand: +
  • coordinates: 2728203..2729141
  • length: 939
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002951 (2728203..2729141) NCBI
  • BioCyc: SACOL_RS13955 BioCyc
  • MicrobesOnline: see SACOL2664

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGCATTATTTTTACAACCCGTTTTTAAAGACAGAATTTGGGGCGGTCATGCATTAAAA
    GCATTTAACTATGATATCCCTAATGAAACAACTGGTGAATGCTGGGCAATTTCTGCACAT
    CCAAATGGTCCTAATACAATTATCAATGGTCCATACAAGGATATGACCCTAGATCAACTT
    TGGTCACAACATCGTGAATTATTCGATAATGATTCACGAGATAGTTTCCCTCTGCTAACT
    AAAGTATTAGATGCCAATGATAAATTATCTGTTCAAGTTCACCCAGATGATGACTACGCT
    TTAAAACACGAAGGCGAACTAGGTAAAACAGAATGTTGGTATATTTTAGATGCAGCCCCA
    GGTGCTGAAATTATATATGGCGTACATGCACATAATAAACAGACGTTAATAGACATGATT
    GATAATCATGAATTTGACAGTCTTTTCAAACGTATACCTGTTAAGCCCGGTGACTTTTAT
    TATGTTCCTGCTGGCACTGTCCATGCCATTGGTTCGGGTATTTTAATTTTAGAGACACAA
    CAATCCTCCGATACGACATACCGTATTTATGACTATGATAGACGAGATCAAAATGGTCAA
    TTGCGTGATTTGCACTTAGAGCAAAGTAAAGATGTGATTGAATTAGGCAATCATGAACCA
    AATACTACTCCTATAACAACTCACATCGACACACACACTGTAACTCAATTTGTAGATAAT
    CAATATTTTGCAGTATATAAATGGGATATTCATGGAGATTTAAAATTCAATAAACCACAT
    GCTTATTGCTTAGTTACAATTATCGATGGTACTGGTACTTTAACAGTAAACGATAAGCAC
    TATGACATCCAAAAAGGTTCTAGCTTTATTTTAACTACGGAAGACGAGGATATTCACTTT
    GTCGGTAATATTAGTGCTATAGTAAGTCATCCTGGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    939

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL_RS13955 [old locus tag: SACOL2664 ]
  • symbol: SACOL_RS13955
  • description: mannose-6-phosphate isomerase, class I
  • length: 312
  • theoretical pI: 4.94021
  • theoretical MW: 35395.2
  • GRAVY: -0.461538

⊟Function[edit | edit source]

  • reaction:
    EC 5.3.1.8?  ExPASy
    Mannose-6-phosphate isomerase D-mannose 6-phosphate = D-fructose 6-phosphate
  • TIGRFAM:
    Metabolism Energy metabolism Sugars mannose-6-phosphate isomerase, class I (TIGR00218; EC 5.3.1.8; HMM-score: 226.2)
  • TheSEED: see SACOL2664
  • PFAM:
    Cupin (CL0029) PMI_typeI; Phosphomannose isomerase type I (PF01238; HMM-score: 158.9)
    and 2 more
    Cupin_2; Cupin domain (PF07883; HMM-score: 37.7)
    AraC_binding; AraC-like ligand binding domain (PF02311; HMM-score: 25)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Zn2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.050223
    • TAT(Tat/SPI): 0.002634
    • LIPO(Sec/SPII): 0.008036
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MALFLQPVFKDRIWGGHALKAFNYDIPNETTGECWAISAHPNGPNTIINGPYKDMTLDQLWSQHRELFDNDSRDSFPLLTKVLDANDKLSVQVHPDDDYALKHEGELGKTECWYILDAAPGAEIIYGVHAHNKQTLIDMIDNHEFDSLFKRIPVKPGDFYYVPAGTVHAIGSGILILETQQSSDTTYRIYDYDRRDQNGQLRDLHLEQSKDVIELGNHEPNTTPITTHIDTHTVTQFVDNQYFAVYKWDIHGDLKFNKPHAYCLVTIIDGTGTLTVNDKHYDIQKGSSFILTTEDEDIHFVGNISAIVSHPG

⊟Experimental data[edit | edit source]

  • experimentally validated: see SACOL2664
  • protein localization: see SACOL2664
  • quantitative data / protein copy number per cell: see SACOL2664
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SACOL_RS13955&oldid=76831"